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Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis.
Diagn Microbiol Infect Dis. 2006 Apr; 54(4):283-7.DM

Abstract

We developed a colorimetric microtiter plate polymerase chain reaction enzyme immunoassay (PCR-EIA) for the detection of Histoplasma capsulatum in urine. The specificity of the PCR assay was confirmed using H. capsulatum (positive control) and Blastomyces dermatitidis (negative control) isolates. The analytical sensitivity of the PCR assay was determined by testing urine samples spiked with freshly grown H. capsulatum organisms and was 2 yeasts per reaction in urine and 0.2 yeasts per reaction in urine sediment after centrifugation. Fifty-one urine specimens positive for H. capsulatum antigen and 25 urine specimens from healthy volunteers were tested blindly. Patient specimens also were cultured for H. capsulatum. The PCR assay was positive in 4 (7.8%) of 51 urine specimens containing antigen and negative in urine specimens from healthy volunteers. The positive PCR results occurred in 4 of 5 urine specimens that were positive by culture, and each exhibited high level of antigenuria (>20 U). Urine cultures were not positive in 24 urine specimens with an antigenuria of 1-19.9 U, but were positive in 5 of 27 urine specimens with antigenuria >20 U. Thus, positive PCR results in urine specimens correlate with positive culture results, but not with antigenuria. The low sensitivity of this PCR assay in urine limits its use in the diagnosis of disseminated histoplasmosis.

Authors+Show Affiliations

Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. yiwei.tang@vanderbilt.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Validation Study

Language

eng

PubMed ID

16466889

Citation

Tang, Yi-Wei, et al. "Urine Polymerase Chain Reaction Is Not as Sensitive as Urine Antigen for the Diagnosis of Disseminated Histoplasmosis." Diagnostic Microbiology and Infectious Disease, vol. 54, no. 4, 2006, pp. 283-7.
Tang YW, Li H, Durkin MM, et al. Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis. Diagn Microbiol Infect Dis. 2006;54(4):283-7.
Tang, Y. W., Li, H., Durkin, M. M., Sefers, S. E., Meng, S., Connolly, P. A., Stratton, C. W., & Wheat, L. J. (2006). Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis. Diagnostic Microbiology and Infectious Disease, 54(4), 283-7.
Tang YW, et al. Urine Polymerase Chain Reaction Is Not as Sensitive as Urine Antigen for the Diagnosis of Disseminated Histoplasmosis. Diagn Microbiol Infect Dis. 2006;54(4):283-7. PubMed PMID: 16466889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis. AU - Tang,Yi-Wei, AU - Li,Haijing, AU - Durkin,Michelle M, AU - Sefers,Sefers E, AU - Meng,Sufang, AU - Connolly,Patricia A, AU - Stratton,Charles W, AU - Wheat,L Joseph, Y1 - 2006/02/08/ PY - 2005/07/19/received PY - 2005/09/22/revised PY - 2005/10/17/accepted PY - 2006/2/10/pubmed PY - 2006/6/10/medline PY - 2006/2/10/entrez SP - 283 EP - 7 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 54 IS - 4 N2 - We developed a colorimetric microtiter plate polymerase chain reaction enzyme immunoassay (PCR-EIA) for the detection of Histoplasma capsulatum in urine. The specificity of the PCR assay was confirmed using H. capsulatum (positive control) and Blastomyces dermatitidis (negative control) isolates. The analytical sensitivity of the PCR assay was determined by testing urine samples spiked with freshly grown H. capsulatum organisms and was 2 yeasts per reaction in urine and 0.2 yeasts per reaction in urine sediment after centrifugation. Fifty-one urine specimens positive for H. capsulatum antigen and 25 urine specimens from healthy volunteers were tested blindly. Patient specimens also were cultured for H. capsulatum. The PCR assay was positive in 4 (7.8%) of 51 urine specimens containing antigen and negative in urine specimens from healthy volunteers. The positive PCR results occurred in 4 of 5 urine specimens that were positive by culture, and each exhibited high level of antigenuria (>20 U). Urine cultures were not positive in 24 urine specimens with an antigenuria of 1-19.9 U, but were positive in 5 of 27 urine specimens with antigenuria >20 U. Thus, positive PCR results in urine specimens correlate with positive culture results, but not with antigenuria. The low sensitivity of this PCR assay in urine limits its use in the diagnosis of disseminated histoplasmosis. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/16466889/Urine_polymerase_chain_reaction_is_not_as_sensitive_as_urine_antigen_for_the_diagnosis_of_disseminated_histoplasmosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(05)00263-4 DB - PRIME DP - Unbound Medicine ER -