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Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice.
J Am Soc Nephrol. 2006 Mar; 17(3):648-54.JA

Abstract

Conventional silencing of many podocyte-specific genes in mice is associated with embryonic or perinatal lethality. Therefore, it would be of great importance to generate mouse models that allow the modification of genes that are expressed in podocytes at later stages of age. Herein is described a transgenic mouse with doxycycline-inducible podocyte-specific expression of Cre recombinase. For the generation of this binary system, a single transgenic construct that contained two separate genes was used: One encoding the optimized M2 version of the doxycycline-dependent transcription transactivator reverse tetracycline-controlled transcriptional activator (rtTA) under control of the human podocin (NPHS2) promoter and the other encoding the recombinase Cre under control of the rtTA/doxycycline-responsive minimal cytomegalovirus (CMV) Tet operator sequence 7 promotor. Microinjection of the JRC-CRE construct in fertilized oocytes from FVB/N mice resulted in 16 transgenic founders. Double-transgenic offspring from breeding of a selected founder with the Z/AP reporter mouse showed alkaline phosphatase staining only upon doxycycline administration and exclusively in podocytes. These data indicate that this new inducible Cre recombinase mouse line is an excellent tool in conditional, kidney glomerular podocyte-specific gene deletion in adult mice.

Authors+Show Affiliations

Research Program in Molecular Medicine, Biomedicum, University of Helsinki, Helsinki, FI-00014 Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16467448

Citation

Juhila, Juuso, et al. "Podocyte Cell-specific Expression of Doxycycline Inducible Cre Recombinase in Mice." Journal of the American Society of Nephrology : JASN, vol. 17, no. 3, 2006, pp. 648-54.
Juhila J, Roozendaal R, Lassila M, et al. Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice. J Am Soc Nephrol. 2006;17(3):648-54.
Juhila, J., Roozendaal, R., Lassila, M., Verbeek, S. J., & Holthofer, H. (2006). Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice. Journal of the American Society of Nephrology : JASN, 17(3), 648-54.
Juhila J, et al. Podocyte Cell-specific Expression of Doxycycline Inducible Cre Recombinase in Mice. J Am Soc Nephrol. 2006;17(3):648-54. PubMed PMID: 16467448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice. AU - Juhila,Juuso, AU - Roozendaal,Ramon, AU - Lassila,Markus, AU - Verbeek,Sjef J, AU - Holthofer,Harry, Y1 - 2006/02/08/ PY - 2006/2/10/pubmed PY - 2006/7/28/medline PY - 2006/2/10/entrez SP - 648 EP - 54 JF - Journal of the American Society of Nephrology : JASN JO - J Am Soc Nephrol VL - 17 IS - 3 N2 - Conventional silencing of many podocyte-specific genes in mice is associated with embryonic or perinatal lethality. Therefore, it would be of great importance to generate mouse models that allow the modification of genes that are expressed in podocytes at later stages of age. Herein is described a transgenic mouse with doxycycline-inducible podocyte-specific expression of Cre recombinase. For the generation of this binary system, a single transgenic construct that contained two separate genes was used: One encoding the optimized M2 version of the doxycycline-dependent transcription transactivator reverse tetracycline-controlled transcriptional activator (rtTA) under control of the human podocin (NPHS2) promoter and the other encoding the recombinase Cre under control of the rtTA/doxycycline-responsive minimal cytomegalovirus (CMV) Tet operator sequence 7 promotor. Microinjection of the JRC-CRE construct in fertilized oocytes from FVB/N mice resulted in 16 transgenic founders. Double-transgenic offspring from breeding of a selected founder with the Z/AP reporter mouse showed alkaline phosphatase staining only upon doxycycline administration and exclusively in podocytes. These data indicate that this new inducible Cre recombinase mouse line is an excellent tool in conditional, kidney glomerular podocyte-specific gene deletion in adult mice. SN - 1046-6673 UR - https://www.unboundmedicine.com/medline/citation/16467448/Podocyte_cell_specific_expression_of_doxycycline_inducible_Cre_recombinase_in_mice_ L2 - https://jasn.asnjournals.org/cgi/pmidlookup?view=long&pmid=16467448 DB - PRIME DP - Unbound Medicine ER -