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Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes.
Insect Mol Biol. 2006 Feb; 15(1):13-24.IM

Abstract

Midgut expressed alkaline serine proteases of Lepidoptera function in conversion of Bacillus thuringiensis (Bt) protoxin to active toxin, and reduced level of transcript T23 is associated with Ostrinia nubilalis resistance to Dipel Bt formulations. Three groups of trypsin- (OnT25, OnT23, and OnT3) and two chymotrypsin-like (OnC1 and OnC2) cDNAs were isolated from O. nubilalis midgut tissue. Intraspecific groupings are based on cDNA similarity and peptide phylogeny. Derived serine proteases showed a catalytic triad (His, Asp, and Ser; except transcript OnT23a), three substrate specificity-determining residues, and three paired disulphide bonds. RT-PCR indicated all transcripts are expressed in the midgut. Mendelian-inherited genomic markers for loci OnT23, OnT3 and OnC1 will be useful for association of alleles with bioassayed Bt toxin resistance phenotypes.

Authors+Show Affiliations

USDA-ARS, Corn Insects and Crop Genetics Research Unit, Iowa State University, Ames, IA 50010, USA. coates@iastate.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16469064

Citation

Coates, B S., et al. "Sequence Variation in Trypsin- and Chymotrypsin-like cDNAs From the Midgut of Ostrinia Nubilalis: Methods for Allelic Differentiation of Candidate Bacillus Thuringiensis Resistance Genes." Insect Molecular Biology, vol. 15, no. 1, 2006, pp. 13-24.
Coates BS, Hellmich RL, Lewis LC. Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes. Insect Mol Biol. 2006;15(1):13-24.
Coates, B. S., Hellmich, R. L., & Lewis, L. C. (2006). Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes. Insect Molecular Biology, 15(1), 13-24.
Coates BS, Hellmich RL, Lewis LC. Sequence Variation in Trypsin- and Chymotrypsin-like cDNAs From the Midgut of Ostrinia Nubilalis: Methods for Allelic Differentiation of Candidate Bacillus Thuringiensis Resistance Genes. Insect Mol Biol. 2006;15(1):13-24. PubMed PMID: 16469064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes. AU - Coates,B S, AU - Hellmich,R L, AU - Lewis,L C, PY - 2006/2/14/pubmed PY - 2006/6/6/medline PY - 2006/2/14/entrez SP - 13 EP - 24 JF - Insect molecular biology JO - Insect Mol Biol VL - 15 IS - 1 N2 - Midgut expressed alkaline serine proteases of Lepidoptera function in conversion of Bacillus thuringiensis (Bt) protoxin to active toxin, and reduced level of transcript T23 is associated with Ostrinia nubilalis resistance to Dipel Bt formulations. Three groups of trypsin- (OnT25, OnT23, and OnT3) and two chymotrypsin-like (OnC1 and OnC2) cDNAs were isolated from O. nubilalis midgut tissue. Intraspecific groupings are based on cDNA similarity and peptide phylogeny. Derived serine proteases showed a catalytic triad (His, Asp, and Ser; except transcript OnT23a), three substrate specificity-determining residues, and three paired disulphide bonds. RT-PCR indicated all transcripts are expressed in the midgut. Mendelian-inherited genomic markers for loci OnT23, OnT3 and OnC1 will be useful for association of alleles with bioassayed Bt toxin resistance phenotypes. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/16469064/Sequence_variation_in_trypsin__and_chymotrypsin_like_cDNAs_from_the_midgut_of_Ostrinia_nubilalis:_methods_for_allelic_differentiation_of_candidate_Bacillus_thuringiensis_resistance_genes_ L2 - https://doi.org/10.1111/j.1365-2583.2006.00598.x DB - PRIME DP - Unbound Medicine ER -