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Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae).
Parasitology. 2006 Jun; 132(Pt 6):803-14.P

Abstract

A microsporidium was isolated from larvae of Spodoptera litura (Fabricius) collected from Tamil Nadu, India. This microsporidian species is monomorphic, disporous and develops in direct contact with the cytoplasm of the host cell. The nuclear configuration of merogonic and sporogonic stages was diplokaryotic. The merogonic proliferative stage was unusual that normal development with 1, 2 and 4 binucleated forms were common, while large multinucleate meronts containing 8 and 12 small compact horseshoe-like diplokaryotic nuclei were also observed. The fresh spores were typically ovocylindrical in shape, with a mean size of 3.91 x 1.91 microm and the polar filament length was approximately 90 microm. Infection was systemic with mature spores produced in the midgut, nervous tissue, muscles, labial glands, gonads, tracheae, epidermis, Malpighian tubules and, most extensively, fat body tissues. The new isolate was highly pathogenic to S. litura larvae. Host specificity tests performed on 37 non-target hosts of 5 different insect orders revealed that the new isolate is pathogenic only to lepidopteran insects. We sequenced the 16S small subunit rRNA (SSU rRNA) gene of the isolate and compared it with 72 non-redundant microsporidian sequences from the GenBank. Based on the light microscopic studies and phylogenetic analyses, the new isolate is assigned to the genus Nosema. Significant differences in the SSU rRNA sequence were identified when compared with the type species Nosema bombycis and other closely related species viz., Nosema spodopterae. Structural differences were also observed in the 16S SSU rRNA between the new isolate and the two above-mentioned microsporidian pathogens. We conclude that the microsporidian isolate reported here is distinctly different from the other known species and is likely to be a new species.

Authors+Show Affiliations

Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500 076, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16469201

Citation

Johny, S, et al. "Morphological and Molecular Characterization of a New Microsporidian (Protozoa: Microsporidia) Isolated From Spodoptera Litura (Fabricius) (Lepidoptera: Noctuidae)." Parasitology, vol. 132, no. Pt 6, 2006, pp. 803-14.
Johny S, Kanginakudru S, Muralirangan MC, et al. Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Parasitology. 2006;132(Pt 6):803-14.
Johny, S., Kanginakudru, S., Muralirangan, M. C., & Nagaraju, J. (2006). Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Parasitology, 132(Pt 6), 803-14.
Johny S, et al. Morphological and Molecular Characterization of a New Microsporidian (Protozoa: Microsporidia) Isolated From Spodoptera Litura (Fabricius) (Lepidoptera: Noctuidae). Parasitology. 2006;132(Pt 6):803-14. PubMed PMID: 16469201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). AU - Johny,S, AU - Kanginakudru,S, AU - Muralirangan,M C, AU - Nagaraju,J, Y1 - 2006/02/10/ PY - 2005/10/10/received PY - 2005/12/08/revised PY - 2005/12/08/accepted PY - 2006/2/14/pubmed PY - 2006/7/26/medline PY - 2006/2/14/entrez SP - 803 EP - 14 JF - Parasitology JO - Parasitology VL - 132 IS - Pt 6 N2 - A microsporidium was isolated from larvae of Spodoptera litura (Fabricius) collected from Tamil Nadu, India. This microsporidian species is monomorphic, disporous and develops in direct contact with the cytoplasm of the host cell. The nuclear configuration of merogonic and sporogonic stages was diplokaryotic. The merogonic proliferative stage was unusual that normal development with 1, 2 and 4 binucleated forms were common, while large multinucleate meronts containing 8 and 12 small compact horseshoe-like diplokaryotic nuclei were also observed. The fresh spores were typically ovocylindrical in shape, with a mean size of 3.91 x 1.91 microm and the polar filament length was approximately 90 microm. Infection was systemic with mature spores produced in the midgut, nervous tissue, muscles, labial glands, gonads, tracheae, epidermis, Malpighian tubules and, most extensively, fat body tissues. The new isolate was highly pathogenic to S. litura larvae. Host specificity tests performed on 37 non-target hosts of 5 different insect orders revealed that the new isolate is pathogenic only to lepidopteran insects. We sequenced the 16S small subunit rRNA (SSU rRNA) gene of the isolate and compared it with 72 non-redundant microsporidian sequences from the GenBank. Based on the light microscopic studies and phylogenetic analyses, the new isolate is assigned to the genus Nosema. Significant differences in the SSU rRNA sequence were identified when compared with the type species Nosema bombycis and other closely related species viz., Nosema spodopterae. Structural differences were also observed in the 16S SSU rRNA between the new isolate and the two above-mentioned microsporidian pathogens. We conclude that the microsporidian isolate reported here is distinctly different from the other known species and is likely to be a new species. SN - 0031-1820 UR - https://www.unboundmedicine.com/medline/citation/16469201/Morphological_and_molecular_characterization_of_a_new_microsporidian__Protozoa:_Microsporidia__isolated_from_Spodoptera_litura__Fabricius___Lepidoptera:_Noctuidae__ L2 - https://www.cambridge.org/core/product/identifier/S0031182006009863/type/journal_article DB - PRIME DP - Unbound Medicine ER -