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Minocycline inhibits caspase-dependent and -independent cell death pathways and is neuroprotective against hippocampal damage after treatment with kainic acid in mice.
Neurosci Lett. 2006 May 08; 398(3):195-200.NL

Abstract

Although minocycline has been generally thought to have neuroprotective properties, the neuroprotective role of minocycline has not been investigated in the animal model of epilepsy. In this study, we investigated whether minocycline is neuroprotective against kainic acid (KA)-induced cell death through the caspase-dependent or -independent mitochondrial apoptotic pathways. Adult male ICR mice were subjected to seizures by intrahippocampal KA injection with vehicle or with minocycline. For cell death analysis, TdT-mediated dUTP-biotin nick end labeling and cresyl-violet staining were performed. Western blot analysis and immunofluorescent staining for cytochrome c and apoptosis-inducing factor (AIF) were performed. Cell death was reduced in minocycline-treated mice. Cytosolic translocation of cytochrome c and subsequent activation of caspase-3 were diminished by minocycline treatment. AIF nuclear translocation and subsequent large-scale DNA fragmentation were also reduced in minocycline-treated mice. Thus, this study suggests that minocycline inhibits both caspase-dependent and -independent apoptotic pathways and may be neuroprotective against hippocampal damage after KA treatment.

Authors+Show Affiliations

Department of Neurology and Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine, 134 Sinchon-dong, Seodaemun-gu, 120-752 Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16469440

Citation

Heo, Kyoung, et al. "Minocycline Inhibits Caspase-dependent and -independent Cell Death Pathways and Is Neuroprotective Against Hippocampal Damage After Treatment With Kainic Acid in Mice." Neuroscience Letters, vol. 398, no. 3, 2006, pp. 195-200.
Heo K, Cho YJ, Cho KJ, et al. Minocycline inhibits caspase-dependent and -independent cell death pathways and is neuroprotective against hippocampal damage after treatment with kainic acid in mice. Neurosci Lett. 2006;398(3):195-200.
Heo, K., Cho, Y. J., Cho, K. J., Kim, H. W., Kim, H. J., Shin, H. Y., Lee, B. I., & Kim, G. W. (2006). Minocycline inhibits caspase-dependent and -independent cell death pathways and is neuroprotective against hippocampal damage after treatment with kainic acid in mice. Neuroscience Letters, 398(3), 195-200.
Heo K, et al. Minocycline Inhibits Caspase-dependent and -independent Cell Death Pathways and Is Neuroprotective Against Hippocampal Damage After Treatment With Kainic Acid in Mice. Neurosci Lett. 2006 May 8;398(3):195-200. PubMed PMID: 16469440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Minocycline inhibits caspase-dependent and -independent cell death pathways and is neuroprotective against hippocampal damage after treatment with kainic acid in mice. AU - Heo,Kyoung, AU - Cho,Yang-Je, AU - Cho,Kyoung-Joo, AU - Kim,Hyun-Woo, AU - Kim,Hyun-Jung, AU - Shin,Ha Young, AU - Lee,Byung In, AU - Kim,Gyung Whan, Y1 - 2006/02/15/ PY - 2005/11/01/received PY - 2005/12/22/revised PY - 2006/01/12/accepted PY - 2006/2/14/pubmed PY - 2006/6/9/medline PY - 2006/2/14/entrez SP - 195 EP - 200 JF - Neuroscience letters JO - Neurosci Lett VL - 398 IS - 3 N2 - Although minocycline has been generally thought to have neuroprotective properties, the neuroprotective role of minocycline has not been investigated in the animal model of epilepsy. In this study, we investigated whether minocycline is neuroprotective against kainic acid (KA)-induced cell death through the caspase-dependent or -independent mitochondrial apoptotic pathways. Adult male ICR mice were subjected to seizures by intrahippocampal KA injection with vehicle or with minocycline. For cell death analysis, TdT-mediated dUTP-biotin nick end labeling and cresyl-violet staining were performed. Western blot analysis and immunofluorescent staining for cytochrome c and apoptosis-inducing factor (AIF) were performed. Cell death was reduced in minocycline-treated mice. Cytosolic translocation of cytochrome c and subsequent activation of caspase-3 were diminished by minocycline treatment. AIF nuclear translocation and subsequent large-scale DNA fragmentation were also reduced in minocycline-treated mice. Thus, this study suggests that minocycline inhibits both caspase-dependent and -independent apoptotic pathways and may be neuroprotective against hippocampal damage after KA treatment. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/16469440/Minocycline_inhibits_caspase_dependent_and__independent_cell_death_pathways_and_is_neuroprotective_against_hippocampal_damage_after_treatment_with_kainic_acid_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(06)00046-2 DB - PRIME DP - Unbound Medicine ER -