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Lycopene and beta-carotene protect in vivo iron-induced oxidative stress damage in rat prostate.
Braz J Med Biol Res 2006; 39(2):203-10BJ

Abstract

It has been suggested that iron overload may be carcinogenic. In the present study, we evaluated the effect of plasma and prostate carotenoid concentration on oxidative DNA damage in 12-week-old Wistar rats treated with intraperitoneal (ip) ferric nitrilotriacetate (Fe-NTA) (10 mg Fe/kg). Plasma beta-carotene and lycopene concentrations were measured as a function of time after ip injection of carotenoids (10 mg kg(-1) day(-1) beta-carotene or lycopene) in rats. The highest total plasma concentration was reached 3 and 6 h after ip injection of lycopene or beta-carotene, respectively. After 5 days of carotenoid treatment, lycopene and beta-carotene were present in the 0.10-0.51 nmol/g wet tissue range in the prostate. Using a sensitive method to detected 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) by HPLC/EC, the level of 8-oxodGuo in rat prostate DNA was significantly higher (6.3 +/- 0.6 residues/10(6) dGuo) 3 h after Fe-NTA injection compared with control rats (1.7 +/- 0.3 residues/10(6) dGuo). Rats supplemented with lycopene or beta-carotene for 5 days prior to Fe-NTA treatment showed a reduction of about 70% in 8-oxodGuo levels to almost control levels. Compared with control rats, the prostate of Fe-NTA-treated animals showed a 78% increase in malondialdehyde accumulation. Lycopene or beta-carotene pre-treatment almost completely prevented lipid damage. Epidemiological studies have suggested a lower risk of prostate cancer in men reporting a higher consumption of tomato products. However, before associating this effect with tomato sauce constituents, more information is required. The results described here may contribute to the understanding of the protective effects of carotenoids against iron-induced oxidative stress.

Authors+Show Affiliations

Departamento de Fisiologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16470307

Citation

Matos, H R., et al. "Lycopene and Beta-carotene Protect in Vivo Iron-induced Oxidative Stress Damage in Rat Prostate." Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, vol. 39, no. 2, 2006, pp. 203-10.
Matos HR, Marques SA, Gomes OF, et al. Lycopene and beta-carotene protect in vivo iron-induced oxidative stress damage in rat prostate. Braz J Med Biol Res. 2006;39(2):203-10.
Matos, H. R., Marques, S. A., Gomes, O. F., Silva, A. A., Heimann, J. C., Di Mascio, P., & Medeiros, M. H. (2006). Lycopene and beta-carotene protect in vivo iron-induced oxidative stress damage in rat prostate. Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, 39(2), pp. 203-10.
Matos HR, et al. Lycopene and Beta-carotene Protect in Vivo Iron-induced Oxidative Stress Damage in Rat Prostate. Braz J Med Biol Res. 2006;39(2):203-10. PubMed PMID: 16470307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lycopene and beta-carotene protect in vivo iron-induced oxidative stress damage in rat prostate. AU - Matos,H R, AU - Marques,S A, AU - Gomes,O F, AU - Silva,A A, AU - Heimann,J C, AU - Di Mascio,P, AU - Medeiros,M H G, Y1 - 2006/02/02/ PY - 2006/2/14/pubmed PY - 2006/11/1/medline PY - 2006/2/14/entrez SP - 203 EP - 10 JF - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JO - Braz. J. Med. Biol. Res. VL - 39 IS - 2 N2 - It has been suggested that iron overload may be carcinogenic. In the present study, we evaluated the effect of plasma and prostate carotenoid concentration on oxidative DNA damage in 12-week-old Wistar rats treated with intraperitoneal (ip) ferric nitrilotriacetate (Fe-NTA) (10 mg Fe/kg). Plasma beta-carotene and lycopene concentrations were measured as a function of time after ip injection of carotenoids (10 mg kg(-1) day(-1) beta-carotene or lycopene) in rats. The highest total plasma concentration was reached 3 and 6 h after ip injection of lycopene or beta-carotene, respectively. After 5 days of carotenoid treatment, lycopene and beta-carotene were present in the 0.10-0.51 nmol/g wet tissue range in the prostate. Using a sensitive method to detected 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) by HPLC/EC, the level of 8-oxodGuo in rat prostate DNA was significantly higher (6.3 +/- 0.6 residues/10(6) dGuo) 3 h after Fe-NTA injection compared with control rats (1.7 +/- 0.3 residues/10(6) dGuo). Rats supplemented with lycopene or beta-carotene for 5 days prior to Fe-NTA treatment showed a reduction of about 70% in 8-oxodGuo levels to almost control levels. Compared with control rats, the prostate of Fe-NTA-treated animals showed a 78% increase in malondialdehyde accumulation. Lycopene or beta-carotene pre-treatment almost completely prevented lipid damage. Epidemiological studies have suggested a lower risk of prostate cancer in men reporting a higher consumption of tomato products. However, before associating this effect with tomato sauce constituents, more information is required. The results described here may contribute to the understanding of the protective effects of carotenoids against iron-induced oxidative stress. SN - 0100-879X UR - https://www.unboundmedicine.com/medline/citation/16470307/Lycopene_and_beta_carotene_protect_in_vivo_iron_induced_oxidative_stress_damage_in_rat_prostate_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200006&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -