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Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides.
Rapid Commun Mass Spectrom. 2006; 20(5):829-36.RC

Abstract

The atmospheric-pressure matrix-assisted laser desorption/ionisation quadrupole ion trap (AP-MALDI-QIT) analysis of tryptic peptides is reported following capillary liquid chromatographic (LC) separation and direct analysis of a protein digest. Peptide fragments were identified by peptide mass fingerprinting from mass spectrometric data and sequence analysis obtained by tandem mass spectrometry of the principal mass spectral peaks using a data-dependent scanning protocol. These data were compared with those from mass spectrometric analysis using capillary LC/MALDI-time-of-flight (TOF) and capillary LC/electrospray ionisation (ESI)-quadrupole TOF. For all three configurations the resulting data were searched against the MSDB database, using MASCOT and the sequence coverage compared for each technique. Complementary data were obtained using the three techniques.

Authors+Show Affiliations

School of Biomedical and Natural Sciences, Nottingham Trent University, Nottingham NG11 8NS, UK. colin.creaser@ntu.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16470569

Citation

Creaser, Colin S., et al. "Capillary Liquid Chromatography/atmospheric-pressure Matrix-assisted Laser Desorption/ionisation Ion Trap Mass Spectrometry: a Comparison With Liquid Chromatography/matrix-assisted Laser Desorption/ionisation Time-of-flight and Liquid Chromatography/electrospray Ionisation Quadrupole Time-of-flight for the Identification of Tryptic Peptides." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 5, 2006, pp. 829-36.
Creaser CS, Green PS, Kilby PM, et al. Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides. Rapid Commun Mass Spectrom. 2006;20(5):829-36.
Creaser, C. S., Green, P. S., Kilby, P. M., & Ratcliffe, L. (2006). Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides. Rapid Communications in Mass Spectrometry : RCM, 20(5), 829-36.
Creaser CS, et al. Capillary Liquid Chromatography/atmospheric-pressure Matrix-assisted Laser Desorption/ionisation Ion Trap Mass Spectrometry: a Comparison With Liquid Chromatography/matrix-assisted Laser Desorption/ionisation Time-of-flight and Liquid Chromatography/electrospray Ionisation Quadrupole Time-of-flight for the Identification of Tryptic Peptides. Rapid Commun Mass Spectrom. 2006;20(5):829-36. PubMed PMID: 16470569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides. AU - Creaser,Colin S, AU - Green,Philip S, AU - Kilby,Peter M, AU - Ratcliffe,Lucy, PY - 2006/2/14/pubmed PY - 2006/4/7/medline PY - 2006/2/14/entrez SP - 829 EP - 36 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 5 N2 - The atmospheric-pressure matrix-assisted laser desorption/ionisation quadrupole ion trap (AP-MALDI-QIT) analysis of tryptic peptides is reported following capillary liquid chromatographic (LC) separation and direct analysis of a protein digest. Peptide fragments were identified by peptide mass fingerprinting from mass spectrometric data and sequence analysis obtained by tandem mass spectrometry of the principal mass spectral peaks using a data-dependent scanning protocol. These data were compared with those from mass spectrometric analysis using capillary LC/MALDI-time-of-flight (TOF) and capillary LC/electrospray ionisation (ESI)-quadrupole TOF. For all three configurations the resulting data were searched against the MSDB database, using MASCOT and the sequence coverage compared for each technique. Complementary data were obtained using the three techniques. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16470569/Capillary_liquid_chromatography/atmospheric_pressure_matrix_assisted_laser_desorption/ionisation_ion_trap_mass_spectrometry:_a_comparison_with_liquid_chromatography/matrix_assisted_laser_desorption/ionisation_time_of_flight_and_liquid_chromatography/electrospray_ionisation_quadrupole_time_of_flight_for_the_identification_of_tryptic_peptides_ L2 - https://doi.org/10.1002/rcm.2376 DB - PRIME DP - Unbound Medicine ER -