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Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: targeted characterization of specific parts of the glycan structure.
Rapid Commun Mass Spectrom. 2006; 20(6):943-51.RC

Abstract

Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) has been introduced in recent years as a valuable tool for the structural characterization of permethylated oligosaccharides. In this report, we describe the combination of MALDI in-source decay (ISD) with the subsequent TOF/TOF-MS analyses of specific fragments, allowing the detailed characterization of the selected part of the oligosaccharide molecule. Part of the second-generation fragment ions were different from those observed in conventional MALDI-TOF/TOF-MS experiments. Other fragments, which had already been observed in conventional MALDI-TOF/TOF-MS and again showed up in second-generation fragment analysis, could be assigned to specific parts of the molecule. Our approach disclosed different structural features of the oligosaccharides: due to permethylation, the glycosidic linkage fragments allowed the distinction between terminal, monosubstituted and disubstituted monosaccharides and indicated the oligosaccharide sequence. Moreover, substitution positions were deduced based on characteristic cross-ring fragmentation by high-energy collision-induced fragmentation. In conclusion, combination of MALDI-ISD with TOF/TOF-MS allows the detailed characterization of specific moieties of permethylated oligosaccharides and is, therefore, a powerful technique for structural glycomics.

Authors+Show Affiliations

Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. m.wuhrer@lumc.nlNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16470682

Citation

Wuhrer, Manfred, and André M. Deelder. "Matrix-assisted Laser Desorption/ionization In-source Decay Combined With Tandem Time-of-flight Mass Spectrometry of Permethylated Oligosaccharides: Targeted Characterization of Specific Parts of the Glycan Structure." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 6, 2006, pp. 943-51.
Wuhrer M, Deelder AM. Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: targeted characterization of specific parts of the glycan structure. Rapid Commun Mass Spectrom. 2006;20(6):943-51.
Wuhrer, M., & Deelder, A. M. (2006). Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: targeted characterization of specific parts of the glycan structure. Rapid Communications in Mass Spectrometry : RCM, 20(6), 943-51.
Wuhrer M, Deelder AM. Matrix-assisted Laser Desorption/ionization In-source Decay Combined With Tandem Time-of-flight Mass Spectrometry of Permethylated Oligosaccharides: Targeted Characterization of Specific Parts of the Glycan Structure. Rapid Commun Mass Spectrom. 2006;20(6):943-51. PubMed PMID: 16470682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: targeted characterization of specific parts of the glycan structure. AU - Wuhrer,Manfred, AU - Deelder,André M, PY - 2006/2/14/pubmed PY - 2006/4/28/medline PY - 2006/2/14/entrez SP - 943 EP - 51 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 6 N2 - Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) has been introduced in recent years as a valuable tool for the structural characterization of permethylated oligosaccharides. In this report, we describe the combination of MALDI in-source decay (ISD) with the subsequent TOF/TOF-MS analyses of specific fragments, allowing the detailed characterization of the selected part of the oligosaccharide molecule. Part of the second-generation fragment ions were different from those observed in conventional MALDI-TOF/TOF-MS experiments. Other fragments, which had already been observed in conventional MALDI-TOF/TOF-MS and again showed up in second-generation fragment analysis, could be assigned to specific parts of the molecule. Our approach disclosed different structural features of the oligosaccharides: due to permethylation, the glycosidic linkage fragments allowed the distinction between terminal, monosubstituted and disubstituted monosaccharides and indicated the oligosaccharide sequence. Moreover, substitution positions were deduced based on characteristic cross-ring fragmentation by high-energy collision-induced fragmentation. In conclusion, combination of MALDI-ISD with TOF/TOF-MS allows the detailed characterization of specific moieties of permethylated oligosaccharides and is, therefore, a powerful technique for structural glycomics. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16470682/Matrix_assisted_laser_desorption/ionization_in_source_decay_combined_with_tandem_time_of_flight_mass_spectrometry_of_permethylated_oligosaccharides:_targeted_characterization_of_specific_parts_of_the_glycan_structure_ L2 - https://doi.org/10.1002/rcm.2390 DB - PRIME DP - Unbound Medicine ER -