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Effect of perineal cleansing on contamination rate of mid-stream urine culture.
J Pediatr Adolesc Gynecol. 2006 Feb; 19(1):31-4.JP

Abstract

STUDY OBJECTIVE

Urinary tract infection (UTI) and chlamydial or gonococcal urethritis are the most common causes of female dysuria. While chlamydia and gonorrhea can be detected with a nucleic acid amplification test performed on an uncleansed first part voided urine sample, urine cultures to test for UTI require a mid-stream clean caught sample. In order to determine whether collecting two sequential non-clean caught urine samples during the same void to test for chlamydia, gonorrhea, and UTI is a reasonable approach, we assessed the degree to which perineal cleansing reduces bacterial contamination of mid-stream urine cultures.

DESIGN

Experimental study comparing mid-stream urine samples collected with (n = 25) and without (n = 25) perineal cleansing.

SETTING

A university-based adolescent clinic

PARTICIPANTS

We recruited fifty 14-23-year-old (mean 18.5 yrs, SD 2.3) asymptomatic females.

MAIN OUTCOME MEASURE

Perineal flora contamination rate of mid-stream urine cultures collected with and without perineal cleansing.

RESULTS

No culture grew >10(4) colonies of a pathogenic bacterium. Eleven (44%) of the experimental group samples and 9 (36%) of the control samples grew >10(4) colonies of perineal bacterial flora (chi(2) = .33, P = 0.56). Participants' previous experience collecting mid-stream urine was not associated with less bacterial contamination.

CONCLUSIONS

Among this small sample of asymptomatic young women, perineal cleansing did not significantly reduce perineal flora contamination of mid-stream urine cultures. If larger studies of symptomatic young women replicate these findings, young women could collect two sequential urine samples from the same void to test for chlamydia, gonorrhea, and UTI.

Authors+Show Affiliations

Blake DR
Department of Pediatrics, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. Diane.Blake@umassmed.edu
Doherty LF
No affiliation info available

MeSH

AdolescentAdultAntisepsisBacteriuriaColony Count, MicrobialFemaleHumansPerineumSpecimen HandlingUrinary Tract InfectionsUrine

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16472726

Citation

Blake, Diane R., and L F. Doherty. "Effect of Perineal Cleansing On Contamination Rate of Mid-stream Urine Culture." Journal of Pediatric and Adolescent Gynecology, vol. 19, no. 1, 2006, pp. 31-4.
Blake DR, Doherty LF. Effect of perineal cleansing on contamination rate of mid-stream urine culture. J Pediatr Adolesc Gynecol. 2006;19(1):31-4.
Blake, D. R., & Doherty, L. F. (2006). Effect of perineal cleansing on contamination rate of mid-stream urine culture. Journal of Pediatric and Adolescent Gynecology, 19(1), 31-4.
Blake DR, Doherty LF. Effect of Perineal Cleansing On Contamination Rate of Mid-stream Urine Culture. J Pediatr Adolesc Gynecol. 2006;19(1):31-4. PubMed PMID: 16472726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of perineal cleansing on contamination rate of mid-stream urine culture. AU - Blake,Diane R, AU - Doherty,L F, PY - 2006/2/14/pubmed PY - 2006/6/23/medline PY - 2006/2/14/entrez SP - 31 EP - 4 JF - Journal of pediatric and adolescent gynecology JO - J Pediatr Adolesc Gynecol VL - 19 IS - 1 N2 - STUDY OBJECTIVE: Urinary tract infection (UTI) and chlamydial or gonococcal urethritis are the most common causes of female dysuria. While chlamydia and gonorrhea can be detected with a nucleic acid amplification test performed on an uncleansed first part voided urine sample, urine cultures to test for UTI require a mid-stream clean caught sample. In order to determine whether collecting two sequential non-clean caught urine samples during the same void to test for chlamydia, gonorrhea, and UTI is a reasonable approach, we assessed the degree to which perineal cleansing reduces bacterial contamination of mid-stream urine cultures. DESIGN: Experimental study comparing mid-stream urine samples collected with (n = 25) and without (n = 25) perineal cleansing. SETTING: A university-based adolescent clinic PARTICIPANTS: We recruited fifty 14-23-year-old (mean 18.5 yrs, SD 2.3) asymptomatic females. MAIN OUTCOME MEASURE: Perineal flora contamination rate of mid-stream urine cultures collected with and without perineal cleansing. RESULTS: No culture grew >10(4) colonies of a pathogenic bacterium. Eleven (44%) of the experimental group samples and 9 (36%) of the control samples grew >10(4) colonies of perineal bacterial flora (chi(2) = .33, P = 0.56). Participants' previous experience collecting mid-stream urine was not associated with less bacterial contamination. CONCLUSIONS: Among this small sample of asymptomatic young women, perineal cleansing did not significantly reduce perineal flora contamination of mid-stream urine cultures. If larger studies of symptomatic young women replicate these findings, young women could collect two sequential urine samples from the same void to test for chlamydia, gonorrhea, and UTI. SN - 1083-3188 UR - https://www.unboundmedicine.com/medline/citation/16472726/Effect_of_perineal_cleansing_on_contamination_rate_of_mid_stream_urine_culture_ DB - PRIME DP - Unbound Medicine ER -