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Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus.
Brain Res. 2006 Mar 03; 1076(1):1-8.BR

Abstract

We have previously shown that neuroprotective effects of an adenoviral glial cell line-derived neurotrophic factor (GDNF) gene transfer on the lesioned adult rat motoneurons in the nucleus ambiguus. In the present study, we examined neuroprotective effects of adenoviral gene transfer of brain-derived neurotrophic factor (BDNF) or/and GDNF to motoneurons in nucleus ambiguus using an adult rat vagal nerve avulsion model. The animals avulsed and inoculated with adenoviral vectors encoding BDNF (AxCAmBDNFME) or/and GDNF (AxCAhGDNF) showed immunolabeling for BDNF or/and GDNF in the nucleus ambiguus on the treated side, respectively, and expression of virus-induced BDNF or/and GDNF mRNA transcripts in the brainstem tissue that contained the nucleus ambiguus of the treated side. The treatment with AxCAhGDNF or AxCAmBDNFME significantly prevented the loss of vagal motoneurons in comparison to the control; the protective effect of AxCAmBDNFME was greater than that of AxCAhGDNF. The combined treatment with AxCAmBDNFME and AxCAhGDNF acted synergistically and significantly larger number of vagal motoneurons was preserved as compared to either AxCAmBDNFME treatment or AxCAhGDNF treatment. The treatment with AxCAmBDNFME or/and AxCAhGDNF after avulsion also suppressed the activity of nitric oxide synthase in lesioned motoneurons in the nucleus ambiguus. These results indicate that adenovirus-mediated BDNF and GDNF gene transfer may prevent the degeneration of motoneurons in humans after either vagal nerve injury or recurrent laryngeal nerve injury.

Authors+Show Affiliations

Department of Otolaryngology-Head and Neck Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 1608582, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16473328

Citation

Moro, Kazuhisa, et al. "Adenoviral Gene Transfer of BDNF and GDNF Synergistically Prevent Motoneuron Loss in the Nucleus Ambiguus." Brain Research, vol. 1076, no. 1, 2006, pp. 1-8.
Moro K, Shiotani A, Watabe K, et al. Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res. 2006;1076(1):1-8.
Moro, K., Shiotani, A., Watabe, K., Takeda, Y., Saito, K., Mori, Y., & Ogawa, K. (2006). Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Research, 1076(1), 1-8.
Moro K, et al. Adenoviral Gene Transfer of BDNF and GDNF Synergistically Prevent Motoneuron Loss in the Nucleus Ambiguus. Brain Res. 2006 Mar 3;1076(1):1-8. PubMed PMID: 16473328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. AU - Moro,Kazuhisa, AU - Shiotani,Akihiro, AU - Watabe,Kazuhiko, AU - Takeda,Yasuo, AU - Saito,Koichiro, AU - Mori,Yuko, AU - Ogawa,Kaoru, Y1 - 2006/02/13/ PY - 2005/05/11/received PY - 2005/11/04/revised PY - 2005/12/30/accepted PY - 2006/2/14/pubmed PY - 2006/7/26/medline PY - 2006/2/14/entrez SP - 1 EP - 8 JF - Brain research JO - Brain Res. VL - 1076 IS - 1 N2 - We have previously shown that neuroprotective effects of an adenoviral glial cell line-derived neurotrophic factor (GDNF) gene transfer on the lesioned adult rat motoneurons in the nucleus ambiguus. In the present study, we examined neuroprotective effects of adenoviral gene transfer of brain-derived neurotrophic factor (BDNF) or/and GDNF to motoneurons in nucleus ambiguus using an adult rat vagal nerve avulsion model. The animals avulsed and inoculated with adenoviral vectors encoding BDNF (AxCAmBDNFME) or/and GDNF (AxCAhGDNF) showed immunolabeling for BDNF or/and GDNF in the nucleus ambiguus on the treated side, respectively, and expression of virus-induced BDNF or/and GDNF mRNA transcripts in the brainstem tissue that contained the nucleus ambiguus of the treated side. The treatment with AxCAhGDNF or AxCAmBDNFME significantly prevented the loss of vagal motoneurons in comparison to the control; the protective effect of AxCAmBDNFME was greater than that of AxCAhGDNF. The combined treatment with AxCAmBDNFME and AxCAhGDNF acted synergistically and significantly larger number of vagal motoneurons was preserved as compared to either AxCAmBDNFME treatment or AxCAhGDNF treatment. The treatment with AxCAmBDNFME or/and AxCAhGDNF after avulsion also suppressed the activity of nitric oxide synthase in lesioned motoneurons in the nucleus ambiguus. These results indicate that adenovirus-mediated BDNF and GDNF gene transfer may prevent the degeneration of motoneurons in humans after either vagal nerve injury or recurrent laryngeal nerve injury. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/16473328/Adenoviral_gene_transfer_of_BDNF_and_GDNF_synergistically_prevent_motoneuron_loss_in_the_nucleus_ambiguus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(06)00008-4 DB - PRIME DP - Unbound Medicine ER -