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Indirect hemagglutination assay in patients with melioidosis in northern Australia.
Am J Trop Med Hyg. 2006 Feb; 74(2):330-4.AJ

Abstract

Melioidosis is caused by the saprophytic organism Burkholderia pseudomallei. The use of the indirect hemagglutination assay (IHA) has found widespread use in areas endemic for this disease. Using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the Northern Territory of Australia. Based on a threshold titer of 1:40, the sensitivity of the IHA on admission was 56%. Female patients, those with positive blood cultures, and those with pneumonia independently predicted a negative IHA result. Most patients (68%) with negative admission IHA titers subsequently seroconverted. Most patients (92%) with positive admission IHA titers had persistently positive IHA titers. Relapses were not observed in 36 patients who had a negative IHA at least 1 month after admission, irrespective of initial admission IHA. The IHA has limited utility as a diagnostic test for acute disease, and most patients subsequently have persistently positive titers after recovery from illness.

Authors+Show Affiliations

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16474092

Citation

Cheng, Allen C., et al. "Indirect Hemagglutination Assay in Patients With Melioidosis in Northern Australia." The American Journal of Tropical Medicine and Hygiene, vol. 74, no. 2, 2006, pp. 330-4.
Cheng AC, O'brien M, Freeman K, et al. Indirect hemagglutination assay in patients with melioidosis in northern Australia. Am J Trop Med Hyg. 2006;74(2):330-4.
Cheng, A. C., O'brien, M., Freeman, K., Lum, G., & Currie, B. J. (2006). Indirect hemagglutination assay in patients with melioidosis in northern Australia. The American Journal of Tropical Medicine and Hygiene, 74(2), 330-4.
Cheng AC, et al. Indirect Hemagglutination Assay in Patients With Melioidosis in Northern Australia. Am J Trop Med Hyg. 2006;74(2):330-4. PubMed PMID: 16474092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indirect hemagglutination assay in patients with melioidosis in northern Australia. AU - Cheng,Allen C, AU - O'brien,Mathew, AU - Freeman,Kevin, AU - Lum,Gary, AU - Currie,Bart J, PY - 2006/2/14/pubmed PY - 2006/4/21/medline PY - 2006/2/14/entrez SP - 330 EP - 4 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 74 IS - 2 N2 - Melioidosis is caused by the saprophytic organism Burkholderia pseudomallei. The use of the indirect hemagglutination assay (IHA) has found widespread use in areas endemic for this disease. Using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the Northern Territory of Australia. Based on a threshold titer of 1:40, the sensitivity of the IHA on admission was 56%. Female patients, those with positive blood cultures, and those with pneumonia independently predicted a negative IHA result. Most patients (68%) with negative admission IHA titers subsequently seroconverted. Most patients (92%) with positive admission IHA titers had persistently positive IHA titers. Relapses were not observed in 36 patients who had a negative IHA at least 1 month after admission, irrespective of initial admission IHA. The IHA has limited utility as a diagnostic test for acute disease, and most patients subsequently have persistently positive titers after recovery from illness. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/16474092/Indirect_hemagglutination_assay_in_patients_with_melioidosis_in_northern_Australia_ DB - PRIME DP - Unbound Medicine ER -