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pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans.
Exp Cell Res. 2006 May 15; 312(9):1475-83.EC

Abstract

Tissue morphogenesis requires proper interaction between cells and the extracellular matrix (ECM), which is mediated by alphabeta heterodimeric receptor integrin. In Caenorhabditis elegans, integrin signaling is essential for formation of gonad. Here, we probe the role of several integrin-associated molecules in ovulation and cell migration. Function of pat-4/integrin-linked kinase (ILK) and unc-112/Mig-2 was examined using RNA-mediated interference (RNAi). Depletion of these messages caused oocyte accumulation in the proximal gonad and distal tip cells (DTC) migration defects. It was further determined that failed ovulation was due to defective contraction and dilation of somatic gonad structures, including spermatheca and gonad sheath. Actin cytoskeleton in the proximal gonad of RNAi animals appeared disorganized, indicating that RNAi of pat-4 or unc-112 inhibited the overall assembly of actin cytoskeleton in somatic gonad. Taken together, our analysis confirms the role of integrin and integrin-associated proteins in gonad function.

Authors+Show Affiliations

Department of Biology, Baylor University, One Bear Place 97388, Waco, TX 76798, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16476426

Citation

Xu, Xiaojian, et al. "Pat-4/ILK and unc-112/Mig-2 Are Required for Gonad Function in Caenorhabditis Elegans." Experimental Cell Research, vol. 312, no. 9, 2006, pp. 1475-83.
Xu X, Rongali SC, Miles JP, et al. Pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans. Exp Cell Res. 2006;312(9):1475-83.
Xu, X., Rongali, S. C., Miles, J. P., Lee, K. D., & Lee, M. (2006). Pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans. Experimental Cell Research, 312(9), 1475-83.
Xu X, et al. Pat-4/ILK and unc-112/Mig-2 Are Required for Gonad Function in Caenorhabditis Elegans. Exp Cell Res. 2006 May 15;312(9):1475-83. PubMed PMID: 16476426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans. AU - Xu,Xiaojian, AU - Rongali,Sharath C, AU - Miles,Jonathan P, AU - Lee,Kap Duk, AU - Lee,Myeongwoo, Y1 - 2006/02/14/ PY - 2005/10/04/received PY - 2006/01/05/revised PY - 2006/01/06/accepted PY - 2006/2/16/pubmed PY - 2006/7/22/medline PY - 2006/2/16/entrez SP - 1475 EP - 83 JF - Experimental cell research JO - Exp Cell Res VL - 312 IS - 9 N2 - Tissue morphogenesis requires proper interaction between cells and the extracellular matrix (ECM), which is mediated by alphabeta heterodimeric receptor integrin. In Caenorhabditis elegans, integrin signaling is essential for formation of gonad. Here, we probe the role of several integrin-associated molecules in ovulation and cell migration. Function of pat-4/integrin-linked kinase (ILK) and unc-112/Mig-2 was examined using RNA-mediated interference (RNAi). Depletion of these messages caused oocyte accumulation in the proximal gonad and distal tip cells (DTC) migration defects. It was further determined that failed ovulation was due to defective contraction and dilation of somatic gonad structures, including spermatheca and gonad sheath. Actin cytoskeleton in the proximal gonad of RNAi animals appeared disorganized, indicating that RNAi of pat-4 or unc-112 inhibited the overall assembly of actin cytoskeleton in somatic gonad. Taken together, our analysis confirms the role of integrin and integrin-associated proteins in gonad function. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/16476426/pat_4/ILK_and_unc_112/Mig_2_are_required_for_gonad_function_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(06)00011-5 DB - PRIME DP - Unbound Medicine ER -