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Postnatal development of substance P-immunoreaction in the trigeminal caudalis of neonatally capsaicin-treated mice.
Arch Histol Cytol. 2005 Dec; 68(4):311-20.AH

Abstract

The trigeminal subnucleus caudalis (Vc) is a critical relay site for processing nociceptive afferent input from the orofacial area in addition to its modulation by neuroplastic change. Although an administration of capsaicin in neonates induces a selective destruction of substance P (SP)-immunoreactive nerve fibers, little information is available regarding its detailed effects on the Vc, particularly during postnatal development. The present study examined postnatal changes in the distribution of SP in the Vc and trigeminal ganglion (TG) by immunohistochemical techniques in naïve (NV) and neonatally capsaicin-treated (CP) mice, combined with a quantitative analysis. The neonatal mice received a single subcutaneous injection of capsaicin (50 mg/kg) at 48 hours after birth. The neural density of the SP-immunoreaction decreased to approximately a quarter of that in 1-week-old NV mice but increased to three-quarters of that in the NV in the superficial area after postnatal week 2. A double staining with SP and myelin basic protein confirmed the absence of any SP-immunoreaction in the myelinated nerve fibers in both NV and CP mice. The SP-immunoreaction never overlapped with non-peptidergic IB4-labeled neurons in the Vc and TG of either group. Neither the size distribution of SP-positive neurons nor their relative ratio in the TG differed between NV and CP mice at the ages of postnatal weeks 1 and 8. These findings indicate two putative origins for the emergent SP-immunoreaction in the superficial layer of the Vc of the CP mice: the surviving trigeminal neurons with SP against capsaicin treatment and/or intrinsic neurons/interneurons in the Vc without SP under normal conditions.

Authors+Show Affiliations

Division of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16477150

Citation

Aita, Megumi, et al. "Postnatal Development of Substance P-immunoreaction in the Trigeminal Caudalis of Neonatally Capsaicin-treated Mice." Archives of Histology and Cytology, vol. 68, no. 4, 2005, pp. 311-20.
Aita M, Maeda T, Takagi R, et al. Postnatal development of substance P-immunoreaction in the trigeminal caudalis of neonatally capsaicin-treated mice. Arch Histol Cytol. 2005;68(4):311-20.
Aita, M., Maeda, T., Takagi, R., & Seo, K. (2005). Postnatal development of substance P-immunoreaction in the trigeminal caudalis of neonatally capsaicin-treated mice. Archives of Histology and Cytology, 68(4), 311-20.
Aita M, et al. Postnatal Development of Substance P-immunoreaction in the Trigeminal Caudalis of Neonatally Capsaicin-treated Mice. Arch Histol Cytol. 2005;68(4):311-20. PubMed PMID: 16477150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postnatal development of substance P-immunoreaction in the trigeminal caudalis of neonatally capsaicin-treated mice. AU - Aita,Megumi, AU - Maeda,Takeyasu, AU - Takagi,Ritsuo, AU - Seo,Kenji, PY - 2006/2/16/pubmed PY - 2009/1/30/medline PY - 2006/2/16/entrez SP - 311 EP - 20 JF - Archives of histology and cytology JO - Arch. Histol. Cytol. VL - 68 IS - 4 N2 - The trigeminal subnucleus caudalis (Vc) is a critical relay site for processing nociceptive afferent input from the orofacial area in addition to its modulation by neuroplastic change. Although an administration of capsaicin in neonates induces a selective destruction of substance P (SP)-immunoreactive nerve fibers, little information is available regarding its detailed effects on the Vc, particularly during postnatal development. The present study examined postnatal changes in the distribution of SP in the Vc and trigeminal ganglion (TG) by immunohistochemical techniques in naïve (NV) and neonatally capsaicin-treated (CP) mice, combined with a quantitative analysis. The neonatal mice received a single subcutaneous injection of capsaicin (50 mg/kg) at 48 hours after birth. The neural density of the SP-immunoreaction decreased to approximately a quarter of that in 1-week-old NV mice but increased to three-quarters of that in the NV in the superficial area after postnatal week 2. A double staining with SP and myelin basic protein confirmed the absence of any SP-immunoreaction in the myelinated nerve fibers in both NV and CP mice. The SP-immunoreaction never overlapped with non-peptidergic IB4-labeled neurons in the Vc and TG of either group. Neither the size distribution of SP-positive neurons nor their relative ratio in the TG differed between NV and CP mice at the ages of postnatal weeks 1 and 8. These findings indicate two putative origins for the emergent SP-immunoreaction in the superficial layer of the Vc of the CP mice: the surviving trigeminal neurons with SP against capsaicin treatment and/or intrinsic neurons/interneurons in the Vc without SP under normal conditions. SN - 0914-9465 UR - https://www.unboundmedicine.com/medline/citation/16477150/Postnatal_development_of_substance_P_immunoreaction_in_the_trigeminal_caudalis_of_neonatally_capsaicin_treated_mice_ L2 - http://japanlinkcenter.org/JST.JSTAGE/aohc/68.311?from=PubMed DB - PRIME DP - Unbound Medicine ER -