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Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts.
Lett Appl Microbiol. 2006 Mar; 42(3):189-94.LA

Abstract

AIMS

To determine the level of bifidobacteria in bio-yoghurts in the UK, identify the species, and compare the resistance of common Bifidobacterium spp. to acidity and oxidative stress.

METHODS AND RESULTS

A storage trial of bio-yoghurts was carried out to determine the level and survival of bifidobacteria. The 16S rRNA gene targeted PCR was used to identify the species. Acid tolerance was determined by introducing the organisms to pH-adjusted skimmed milk and enumerating during storage at 4 degrees C. Oxidative stress resistance was determined using the H(2)O(2) disc diffusion assay technique. Nine of 10 bio-yoghurts contained bifidobacteria at levels >10(6) CFU g(-1) at the time of purchase. The viability of the organism decreased during storage and on expiry only five products retained viability >10(6) CFU g(-1) while two others were very close to the target population. Bifidobacterium animalis ssp. lactis showed superior survival abilities and stress tolerance compared with Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium adolescentis and Bifidobacterium longum biotype infantis.

CONCLUSIONS

Bifidobacterium animalis ssp. lactis, the only Bifidobacterium spp. found in bio-yoghurts, had the greatest resistance to acidity and oxidative stress.

SIGNIFICANCE AND IMPACT OF THE STUDY

The technological properties of B. animalis ssp. lactis make it suitable for inclusion in bio-yoghurts although its putative health benefits need further investigation.

Authors+Show Affiliations

School of Biomedical and Molecular Sciences, University of Surrey, Guildford, UK.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16478503

Citation

Jayamanne, V S., and M R. Adams. "Determination of Survival, Identity and Stress Resistance of Probiotic Bifidobacteria in Bio-yoghurts." Letters in Applied Microbiology, vol. 42, no. 3, 2006, pp. 189-94.
Jayamanne VS, Adams MR. Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. Lett Appl Microbiol. 2006;42(3):189-94.
Jayamanne, V. S., & Adams, M. R. (2006). Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. Letters in Applied Microbiology, 42(3), 189-94.
Jayamanne VS, Adams MR. Determination of Survival, Identity and Stress Resistance of Probiotic Bifidobacteria in Bio-yoghurts. Lett Appl Microbiol. 2006;42(3):189-94. PubMed PMID: 16478503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. AU - Jayamanne,V S, AU - Adams,M R, PY - 2006/2/16/pubmed PY - 2006/5/5/medline PY - 2006/2/16/entrez SP - 189 EP - 94 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 42 IS - 3 N2 - AIMS: To determine the level of bifidobacteria in bio-yoghurts in the UK, identify the species, and compare the resistance of common Bifidobacterium spp. to acidity and oxidative stress. METHODS AND RESULTS: A storage trial of bio-yoghurts was carried out to determine the level and survival of bifidobacteria. The 16S rRNA gene targeted PCR was used to identify the species. Acid tolerance was determined by introducing the organisms to pH-adjusted skimmed milk and enumerating during storage at 4 degrees C. Oxidative stress resistance was determined using the H(2)O(2) disc diffusion assay technique. Nine of 10 bio-yoghurts contained bifidobacteria at levels >10(6) CFU g(-1) at the time of purchase. The viability of the organism decreased during storage and on expiry only five products retained viability >10(6) CFU g(-1) while two others were very close to the target population. Bifidobacterium animalis ssp. lactis showed superior survival abilities and stress tolerance compared with Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium adolescentis and Bifidobacterium longum biotype infantis. CONCLUSIONS: Bifidobacterium animalis ssp. lactis, the only Bifidobacterium spp. found in bio-yoghurts, had the greatest resistance to acidity and oxidative stress. SIGNIFICANCE AND IMPACT OF THE STUDY: The technological properties of B. animalis ssp. lactis make it suitable for inclusion in bio-yoghurts although its putative health benefits need further investigation. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/16478503/Determination_of_survival_identity_and_stress_resistance_of_probiotic_bifidobacteria_in_bio_yoghurts_ L2 - https://doi.org/10.1111/j.1472-765X.2006.01843.x DB - PRIME DP - Unbound Medicine ER -