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Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens.
Poult Sci. 2005 Dec; 84(12):1886-92.PS

Abstract

A total of 216 Brown Dwarf laying hens (1.62 +/- 0.06 kg BW and 60 wk old) were fed 1 of 3 corn-soybean meal-based diets containing 0, 2.5, or 5.0% conjugated linoleic aicd (CLA) to explore its effects on the fatty acid composition of egg yolk, plasma, and liver as well as hepatic stearoyl-coenzyme A desaturase-1 (SCD-1) activity and its mRNA gene expression. Four hens were placed in wired-floored cages (45 x 40 x 45 cm) and 3 cages were grouped as 1 replicate, resulting in 6 replicates per treatment. The experimental diets were fed for 54 d, and then eggs were collected to determine the fatty acid composition of egg yolk. Four eggs were randomly selected from the total day's production for each replicate, and the contents were pooled prior to analysis. On d 56, one randomly chosen hen from each replicate (6 hens per replicate and a total of 18 hens) was bled via heart puncture and then killed in order to collect liver samples to measure the fatty acid profile of plasma and liver tissue as well as hepatic SCD-1 activity and its mRNA abundance. Dietary supplementation of CLA resulted in a significant deposition of CLA in egg yolk, plasma, and liver lipids (P < 0.01). As the dietary level of CLA increased, the concentration of saturated fatty acids in egg yolk, plasma, and liver also increased (P < 0.05). However, the concentration of monounsaturated fatty acids in these same tissues decreased (P < 0.01). Compared with the control, the activity of SCD-1 was reduced by feeding 2.5% CLA (P < 0.05) without a change in SCD-1 mRNA gene expression. However, feeding 5% CLA reduced both SCD-1 activity and mRNA abundance (P < 0.05). These results indicate that the conversion of saturated to monounsaturated fatty acids in egg yolk, plasma, and liver might be modulated directly at hepatic mRNA gene expression levels, or may be indirectly regulated at the downstream post-transcriptional levels.

Authors+Show Affiliations

National Key Lab of Animal Nutrition, China Agricultural University, Beijing, People's Republic of China 100094.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

16479945

Citation

Shang, X G., et al. "Effect of Dietary Conjugated Linoleic Acid On the Fatty Acid Composition of Egg Yolk, Plasma and Liver as Well as Hepatic Stearoyl-coenzyme a Desaturase Activity and Gene Expression in Laying Hens." Poultry Science, vol. 84, no. 12, 2005, pp. 1886-92.
Shang XG, Wang FL, Li DF, et al. Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens. Poult Sci. 2005;84(12):1886-92.
Shang, X. G., Wang, F. L., Li, D. F., Yin, J. D., Li, X. J., & Yi, G. F. (2005). Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens. Poultry Science, 84(12), 1886-92.
Shang XG, et al. Effect of Dietary Conjugated Linoleic Acid On the Fatty Acid Composition of Egg Yolk, Plasma and Liver as Well as Hepatic Stearoyl-coenzyme a Desaturase Activity and Gene Expression in Laying Hens. Poult Sci. 2005;84(12):1886-92. PubMed PMID: 16479945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk, plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens. AU - Shang,X G, AU - Wang,F L, AU - Li,D F, AU - Yin,J D, AU - Li,X J, AU - Yi,G F, PY - 2006/2/17/pubmed PY - 2006/4/14/medline PY - 2006/2/17/entrez SP - 1886 EP - 92 JF - Poultry science JO - Poult Sci VL - 84 IS - 12 N2 - A total of 216 Brown Dwarf laying hens (1.62 +/- 0.06 kg BW and 60 wk old) were fed 1 of 3 corn-soybean meal-based diets containing 0, 2.5, or 5.0% conjugated linoleic aicd (CLA) to explore its effects on the fatty acid composition of egg yolk, plasma, and liver as well as hepatic stearoyl-coenzyme A desaturase-1 (SCD-1) activity and its mRNA gene expression. Four hens were placed in wired-floored cages (45 x 40 x 45 cm) and 3 cages were grouped as 1 replicate, resulting in 6 replicates per treatment. The experimental diets were fed for 54 d, and then eggs were collected to determine the fatty acid composition of egg yolk. Four eggs were randomly selected from the total day's production for each replicate, and the contents were pooled prior to analysis. On d 56, one randomly chosen hen from each replicate (6 hens per replicate and a total of 18 hens) was bled via heart puncture and then killed in order to collect liver samples to measure the fatty acid profile of plasma and liver tissue as well as hepatic SCD-1 activity and its mRNA abundance. Dietary supplementation of CLA resulted in a significant deposition of CLA in egg yolk, plasma, and liver lipids (P < 0.01). As the dietary level of CLA increased, the concentration of saturated fatty acids in egg yolk, plasma, and liver also increased (P < 0.05). However, the concentration of monounsaturated fatty acids in these same tissues decreased (P < 0.01). Compared with the control, the activity of SCD-1 was reduced by feeding 2.5% CLA (P < 0.05) without a change in SCD-1 mRNA gene expression. However, feeding 5% CLA reduced both SCD-1 activity and mRNA abundance (P < 0.05). These results indicate that the conversion of saturated to monounsaturated fatty acids in egg yolk, plasma, and liver might be modulated directly at hepatic mRNA gene expression levels, or may be indirectly regulated at the downstream post-transcriptional levels. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/16479945/Effect_of_dietary_conjugated_linoleic_acid_on_the_fatty_acid_composition_of_egg_yolk_plasma_and_liver_as_well_as_hepatic_stearoyl_coenzyme_A_desaturase_activity_and_gene_expression_in_laying_hens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)44748-2 DB - PRIME DP - Unbound Medicine ER -