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Protective effect of pyrrolidine dithiocarbamate on liver injury induced by intestinal ischemia-reperfusion in rats.
Hepatobiliary Pancreat Dis Int. 2006 Feb; 5(1):90-5.HP

Abstract

BACKGROUND

The nuclear translocation of transcription factors may be a critical factor in the intracellular pathway involved in ischemia/reperfusion (I/R) injury. The aim of the study was to evaluate the role of nuclear factor-kappa B (NF-kappaB) in the pathogenesis of liver injury induced by intestinal ischemia/reperfusion (IIR) and to investigate the effect of pyrrolidine dithiocarbamate (PDTC) on this liver injury.

METHODS

Male Wistar rats were divided randomly into three experimental groups (8 rats in each): sham operation group (control group); intestinal/reperfusion group (I/R group): animals received 1-hour of intestinal ischemia and 2-hour reperfusion; and PDTC treatment group (PDTC group): animals that received I/R subject to PDTC treatment (100 mg/kg). The histological changes in the liver and intestine were observed, and the serum levels of tumor necrosis factor-alpha (TNF-alpha), alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver superoxide dismutase (SOD), and nitrite/nitrate (NO) were measured. The immunohistochemical expression and Western blot analysis of liver NF-kappaB and intercellular adhesion molecule-1 (ICAM-1) were observed.

RESULTS

IIR induced liver injury characterized by the histological changes of liver edema, hemorrhage, polymorphonuclear neutrophil (PMN) infiltration, and elevated serum levels of AST and ALT. The serum TNF-alpha level was significantly higher than that of the control group (P<0.01) and a high level of liver oxidant product was observed (P<0.01). These changes were parallel to the positive expression of NF-kappaB and ICAM-1. After the administration of PDTC, the histological changes after liver injury were improved; the levels of SOD and NO in the liver were elevated and reduced, respectively (P<0.01). The expressions of ICAM-1 and NF-kappaB in the liver were weakened (P<0.01).

CONCLUSION

NF-kappaB plays an important role in the pathogenesis of liver injury induced by IIR. PDTC, an agent known to inhibit the activation of NF-kappaB, can reduce and prevent this injury.

Authors+Show Affiliations

Department of General Surgery, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. txfdl@tom.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16481291

Citation

Tian, Xiao-Feng, et al. "Protective Effect of Pyrrolidine Dithiocarbamate On Liver Injury Induced By Intestinal Ischemia-reperfusion in Rats." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 5, no. 1, 2006, pp. 90-5.
Tian XF, Yao JH, Li YH, et al. Protective effect of pyrrolidine dithiocarbamate on liver injury induced by intestinal ischemia-reperfusion in rats. Hepatobiliary Pancreat Dis Int. 2006;5(1):90-5.
Tian, X. F., Yao, J. H., Li, Y. H., Gao, H. F., Wang, Z. Z., Yang, C. M., & Zheng, S. S. (2006). Protective effect of pyrrolidine dithiocarbamate on liver injury induced by intestinal ischemia-reperfusion in rats. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 5(1), 90-5.
Tian XF, et al. Protective Effect of Pyrrolidine Dithiocarbamate On Liver Injury Induced By Intestinal Ischemia-reperfusion in Rats. Hepatobiliary Pancreat Dis Int. 2006;5(1):90-5. PubMed PMID: 16481291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of pyrrolidine dithiocarbamate on liver injury induced by intestinal ischemia-reperfusion in rats. AU - Tian,Xiao-Feng, AU - Yao,Ji-Hong, AU - Li,Ying-Hua, AU - Gao,Hai-Feng, AU - Wang,Zhen-Zhen, AU - Yang,Chun-Ming, AU - Zheng,Shu-Sen, PY - 2006/2/17/pubmed PY - 2006/6/16/medline PY - 2006/2/17/entrez SP - 90 EP - 5 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - Hepatobiliary Pancreat Dis Int VL - 5 IS - 1 N2 - BACKGROUND: The nuclear translocation of transcription factors may be a critical factor in the intracellular pathway involved in ischemia/reperfusion (I/R) injury. The aim of the study was to evaluate the role of nuclear factor-kappa B (NF-kappaB) in the pathogenesis of liver injury induced by intestinal ischemia/reperfusion (IIR) and to investigate the effect of pyrrolidine dithiocarbamate (PDTC) on this liver injury. METHODS: Male Wistar rats were divided randomly into three experimental groups (8 rats in each): sham operation group (control group); intestinal/reperfusion group (I/R group): animals received 1-hour of intestinal ischemia and 2-hour reperfusion; and PDTC treatment group (PDTC group): animals that received I/R subject to PDTC treatment (100 mg/kg). The histological changes in the liver and intestine were observed, and the serum levels of tumor necrosis factor-alpha (TNF-alpha), alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver superoxide dismutase (SOD), and nitrite/nitrate (NO) were measured. The immunohistochemical expression and Western blot analysis of liver NF-kappaB and intercellular adhesion molecule-1 (ICAM-1) were observed. RESULTS: IIR induced liver injury characterized by the histological changes of liver edema, hemorrhage, polymorphonuclear neutrophil (PMN) infiltration, and elevated serum levels of AST and ALT. The serum TNF-alpha level was significantly higher than that of the control group (P<0.01) and a high level of liver oxidant product was observed (P<0.01). These changes were parallel to the positive expression of NF-kappaB and ICAM-1. After the administration of PDTC, the histological changes after liver injury were improved; the levels of SOD and NO in the liver were elevated and reduced, respectively (P<0.01). The expressions of ICAM-1 and NF-kappaB in the liver were weakened (P<0.01). CONCLUSION: NF-kappaB plays an important role in the pathogenesis of liver injury induced by IIR. PDTC, an agent known to inhibit the activation of NF-kappaB, can reduce and prevent this injury. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/16481291/Protective_effect_of_pyrrolidine_dithiocarbamate_on_liver_injury_induced_by_intestinal_ischemia_reperfusion_in_rats_ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -