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Cloning and sequence analysis of the 22-kDa antigen genes of Orientia tsutsugamushi strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119.
Ann N Y Acad Sci. 2005 Dec; 1063:231-8.AN

Abstract

The 22-kDa protein antigen is one of several antigens recognized by sera from scrub typhus patients infected with Orientia tsutsugamushi. The 22-kDa protein genes from six O. tsutsugamushi strains (Kato, TA763, AFSC 7, 18-032460, TH1814, MAK119) were cloned and their sequences were determined and compared to each other and to the Karp strain sequence listed in GenBank. The sequence alignment revealed that the promoter regions of these seven strains were highly conserved. However, the ORFs exhibited some variation. The phylogenetic analysis of the DNA sequences indicated that among the seven strains assessed, Kato and TA763 were the most closely related, while Karp and TH1814 were the most distantly related. The information gained from this analysis will facilitate our selection of O. tsutsugamushi strains from which antigens should be derived to be included in a multivalent vaccine candidate for scrub typhus.

Authors+Show Affiliations

Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910, USA. geh@nmrc.navy.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16481519

Citation

Ge, Hong, et al. "Cloning and Sequence Analysis of the 22-kDa Antigen Genes of Orientia Tsutsugamushi Strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119." Annals of the New York Academy of Sciences, vol. 1063, 2005, pp. 231-8.
Ge H, Tong M, Li A, et al. Cloning and sequence analysis of the 22-kDa antigen genes of Orientia tsutsugamushi strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119. Ann N Y Acad Sci. 2005;1063:231-8.
Ge, H., Tong, M., Li, A., Mehta, R., & Ching, W. M. (2005). Cloning and sequence analysis of the 22-kDa antigen genes of Orientia tsutsugamushi strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119. Annals of the New York Academy of Sciences, 1063, 231-8.
Ge H, et al. Cloning and Sequence Analysis of the 22-kDa Antigen Genes of Orientia Tsutsugamushi Strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119. Ann N Y Acad Sci. 2005;1063:231-8. PubMed PMID: 16481519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and sequence analysis of the 22-kDa antigen genes of Orientia tsutsugamushi strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119. AU - Ge,Hong, AU - Tong,Min, AU - Li,Andrew, AU - Mehta,Rajan, AU - Ching,Wei-Mei, PY - 2006/2/17/pubmed PY - 2006/7/13/medline PY - 2006/2/17/entrez SP - 231 EP - 8 JF - Annals of the New York Academy of Sciences JO - Ann N Y Acad Sci VL - 1063 N2 - The 22-kDa protein antigen is one of several antigens recognized by sera from scrub typhus patients infected with Orientia tsutsugamushi. The 22-kDa protein genes from six O. tsutsugamushi strains (Kato, TA763, AFSC 7, 18-032460, TH1814, MAK119) were cloned and their sequences were determined and compared to each other and to the Karp strain sequence listed in GenBank. The sequence alignment revealed that the promoter regions of these seven strains were highly conserved. However, the ORFs exhibited some variation. The phylogenetic analysis of the DNA sequences indicated that among the seven strains assessed, Kato and TA763 were the most closely related, while Karp and TH1814 were the most distantly related. The information gained from this analysis will facilitate our selection of O. tsutsugamushi strains from which antigens should be derived to be included in a multivalent vaccine candidate for scrub typhus. SN - 0077-8923 UR - https://www.unboundmedicine.com/medline/citation/16481519/Cloning_and_sequence_analysis_of_the_22_kDa_antigen_genes_of_Orientia_tsutsugamushi_strains_Kato_TA763_AFSC_7_18_032460_TH1814_and_MAK_119_ L2 - https://doi.org/10.1196/annals.1355.036 DB - PRIME DP - Unbound Medicine ER -