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In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice.
J Orthop Res. 2006 Mar; 24(3):301-12.JO

Abstract

Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow and possess the potential to differentiate into progenies of embryonic mesoderm. However, current evidence is based predominantly on in vitro experiments. We used a murine model of in utero transplantation (IUT) to study the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow by negative immunoselection and limiting dilution, and were characterized by flow cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 days. Engraftment of human MSCs was determined by flow cytometry, polymerase chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted into tissues originating from all three germ layers and persisted for up to 4 months or more after delivery, as evidenced by the expression of the human-specific beta-2 microglobulin gene and by FISH for donor-derived cells. Donor-derived CD45+ cells were detectable in the peripheral blood of recipients, suggesting the participation of MSCs in hematopoiesis at the fetal stage. This model can further serve to evaluate possible applications of MSCs.

Authors+Show Affiliations

Department of Life Science, Fu-Jen University, 510 Zhongzheng Road, Hsinehuang City, Taipei 242, Taiwan, Republic of China. bio0004@mails.fju.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16482576

Citation

Chou, Shiu-Huey, et al. "In Utero Transplantation of Human Bone Marrow-derived Multipotent Mesenchymal Stem Cells in Mice." Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, vol. 24, no. 3, 2006, pp. 301-12.
Chou SH, Kuo TK, Liu M, et al. In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice. J Orthop Res. 2006;24(3):301-12.
Chou, S. H., Kuo, T. K., Liu, M., & Lee, O. K. (2006). In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice. Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 24(3), 301-12.
Chou SH, et al. In Utero Transplantation of Human Bone Marrow-derived Multipotent Mesenchymal Stem Cells in Mice. J Orthop Res. 2006;24(3):301-12. PubMed PMID: 16482576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice. AU - Chou,Shiu-Huey, AU - Kuo,Tom K, AU - Liu,Ming, AU - Lee,Oscar K, PY - 2006/2/17/pubmed PY - 2006/4/14/medline PY - 2006/2/17/entrez SP - 301 EP - 12 JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society JO - J. Orthop. Res. VL - 24 IS - 3 N2 - Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow and possess the potential to differentiate into progenies of embryonic mesoderm. However, current evidence is based predominantly on in vitro experiments. We used a murine model of in utero transplantation (IUT) to study the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow by negative immunoselection and limiting dilution, and were characterized by flow cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 days. Engraftment of human MSCs was determined by flow cytometry, polymerase chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted into tissues originating from all three germ layers and persisted for up to 4 months or more after delivery, as evidenced by the expression of the human-specific beta-2 microglobulin gene and by FISH for donor-derived cells. Donor-derived CD45+ cells were detectable in the peripheral blood of recipients, suggesting the participation of MSCs in hematopoiesis at the fetal stage. This model can further serve to evaluate possible applications of MSCs. SN - 0736-0266 UR - https://www.unboundmedicine.com/medline/citation/16482576/In_utero_transplantation_of_human_bone_marrow_derived_multipotent_mesenchymal_stem_cells_in_mice_ L2 - https://doi.org/10.1002/jor.20047 DB - PRIME DP - Unbound Medicine ER -