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Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis.
Am J Clin Pathol. 2006 Jan; 125(1):42-8.AJ

Abstract

To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglobulin heavy chain (IgH) and bcl-2/IgH fusion genes using DNA extracted from aspirate smears and/or paraffin-embedded tissues. By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT.

Authors+Show Affiliations

Department of Pathology, Baylor College of Medicine, Houston, TX, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16482990

Citation

Chen, Henry I., et al. "Restricted Kappa/lambda Light Chain Ratio By Flow Cytometry in Germinal Center B Cells in Hashimoto Thyroiditis." American Journal of Clinical Pathology, vol. 125, no. 1, 2006, pp. 42-8.
Chen HI, Akpolat I, Mody DR, et al. Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis. Am J Clin Pathol. 2006;125(1):42-8.
Chen, H. I., Akpolat, I., Mody, D. R., Lopez-Terrada, D., De Leon, A. P., Luo, Y., Jorgensen, J., Schwartz, M. R., & Chang, C. C. (2006). Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis. American Journal of Clinical Pathology, 125(1), 42-8.
Chen HI, et al. Restricted Kappa/lambda Light Chain Ratio By Flow Cytometry in Germinal Center B Cells in Hashimoto Thyroiditis. Am J Clin Pathol. 2006;125(1):42-8. PubMed PMID: 16482990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis. AU - Chen,Henry I, AU - Akpolat,Ikser, AU - Mody,Dina R, AU - Lopez-Terrada,Dolores, AU - De Leon,Audrey Ponce, AU - Luo,Yamin, AU - Jorgensen,Jeffrey, AU - Schwartz,Mary R, AU - Chang,Chung-Che, PY - 2006/2/18/pubmed PY - 2006/3/10/medline PY - 2006/2/18/entrez SP - 42 EP - 8 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 125 IS - 1 N2 - To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglobulin heavy chain (IgH) and bcl-2/IgH fusion genes using DNA extracted from aspirate smears and/or paraffin-embedded tissues. By flow cytometric analysis, the CD10+ GC B cells had a higher mean +/- SD kappa/lambda ratio than the CD10- B cells (5.1 +/- 3.3 vs 2.0 +/- 0.8; P < .0001, Student t test). In 18 samples (67%), CD10+ GC B cells had a kappa/lambda ratio greater than 3.07 (the upper limit of kappa/lambda ratio reported in reactive nodes; range, 3.2-14.4 in the 18 cases). Cases tested by PCR showed no evidence of a clonal proliferation. None of 21 cases developed lymphoma during clinical follow-up of up to 3 years. The kappa/lambda ratio of CD10+ GC B cells in HT can be skewed markedly beyond that reported in reactive lymph nodes. This finding frequently is present in HT. Pathologists should be familiar with this phenomenon to prevent misdiagnosis of follicular lymphoma in patients with HT. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/16482990/Restricted_kappa/lambda_light_chain_ratio_by_flow_cytometry_in_germinal_center_B_cells_in_Hashimoto_thyroiditis_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=16482990.ui DB - PRIME DP - Unbound Medicine ER -