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Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase.
J Bacteriol. 1991 Jul; 173(14):4347-52.JB

Abstract

Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related transposons involves excision of a free, nonreplicative, covalently closed circular intermediate that is substrate for integration. Excisive recombination requires two transposon-encoded proteins, Xis-Tn and Int-Tn, whereas the latter protein alone is sufficient for integration. Here we report that conjugative transposition of Tn916 requires the presence of a functional integrase in both donor and recipient strains. We have constructed a mutant, designated Tn916-int1, by replacing the gene directing synthesis of Int-Tn by an allele inactivated in vitro. In mating experiments, transfer of Tn916-int1 from Bacillus subtilis to E. faecalis was detected only when the transposon-encoded integrase was supplied by trans-complementation in both the donor and the recipient. These results suggest that conjugative transposition of Tn916 requires circularization of the element in the donor followed by transfer and integration of the nonreplicative intermediate in the recipient.

Authors+Show Affiliations

Unité des Agents Antibactériens, Institut Pasteur, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1648556

Citation

Storrs, M J., et al. "Conjugative Transposition of Tn916 Requires the Excisive and Integrative Activities of the Transposon-encoded Integrase." Journal of Bacteriology, vol. 173, no. 14, 1991, pp. 4347-52.
Storrs MJ, Poyart-Salmeron C, Trieu-Cuot P, et al. Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase. J Bacteriol. 1991;173(14):4347-52.
Storrs, M. J., Poyart-Salmeron, C., Trieu-Cuot, P., & Courvalin, P. (1991). Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase. Journal of Bacteriology, 173(14), 4347-52.
Storrs MJ, et al. Conjugative Transposition of Tn916 Requires the Excisive and Integrative Activities of the Transposon-encoded Integrase. J Bacteriol. 1991;173(14):4347-52. PubMed PMID: 1648556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase. AU - Storrs,M J, AU - Poyart-Salmeron,C, AU - Trieu-Cuot,P, AU - Courvalin,P, PY - 1991/7/1/pubmed PY - 1991/7/1/medline PY - 1991/7/1/entrez SP - 4347 EP - 52 JF - Journal of bacteriology JO - J. Bacteriol. VL - 173 IS - 14 N2 - Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related transposons involves excision of a free, nonreplicative, covalently closed circular intermediate that is substrate for integration. Excisive recombination requires two transposon-encoded proteins, Xis-Tn and Int-Tn, whereas the latter protein alone is sufficient for integration. Here we report that conjugative transposition of Tn916 requires the presence of a functional integrase in both donor and recipient strains. We have constructed a mutant, designated Tn916-int1, by replacing the gene directing synthesis of Int-Tn by an allele inactivated in vitro. In mating experiments, transfer of Tn916-int1 from Bacillus subtilis to E. faecalis was detected only when the transposon-encoded integrase was supplied by trans-complementation in both the donor and the recipient. These results suggest that conjugative transposition of Tn916 requires circularization of the element in the donor followed by transfer and integration of the nonreplicative intermediate in the recipient. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/1648556/Conjugative_transposition_of_Tn916_requires_the_excisive_and_integrative_activities_of_the_transposon_encoded_integrase_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=1648556 DB - PRIME DP - Unbound Medicine ER -