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Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 03; 833(2):186-90.JC

Abstract

A simple and reliable capillary electrophoresis (CE) method with UV-vis detection is presented for the enantioselective separation and determination of vigabatrin enantiomers. Dehydroabietylisothiocyante (DHAIC), a novel chiral derivatizing reagent, was used for precolumn derivatization of vigabatrin enantiomers. Optimal separation was obtained with a running buffer consisting of 50 mM Na2HPO4 (pH 9.0), 17 mM sodium dodecyl sulfate (SDS) and 25% acetonitrile. The enantiomeric separation of vigabatrin derivatives was achieved within 25 min, and the resolution was found to be 2.1. Detection was followed by direct UV absorptiometric measurements at 202 nm. A calibration curve ranging from 0.3 to 6.0 microg/ml was shown to be linear, and the limit of detection was 0.15 microg/ml. The developed method has been applied to the determination of vigabatrin enantiomers spiked in human plasma, no interferences were found from endogenous amino acids.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16488672

Citation

Zhao, Shulin, et al. "Capillary Electrophoresis Enantioselective Separation of Vigabatrin Enantiomers By Precolumn Derivatization With Dehydroabietylisothiocyante and UV-vis Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 833, no. 2, 2006, pp. 186-90.
Zhao S, Zhang R, Wang H, et al. Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;833(2):186-90.
Zhao, S., Zhang, R., Wang, H., Tang, L., & Pan, Y. (2006). Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 833(2), 186-90.
Zhao S, et al. Capillary Electrophoresis Enantioselective Separation of Vigabatrin Enantiomers By Precolumn Derivatization With Dehydroabietylisothiocyante and UV-vis Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 3;833(2):186-90. PubMed PMID: 16488672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection. AU - Zhao,Shulin, AU - Zhang,Rongcan, AU - Wang,Hengshan, AU - Tang,Lidong, AU - Pan,Yinming, Y1 - 2006/02/20/ PY - 2005/10/29/received PY - 2006/01/15/revised PY - 2006/01/21/accepted PY - 2006/2/21/pubmed PY - 2006/7/18/medline PY - 2006/2/21/entrez SP - 186 EP - 90 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 833 IS - 2 N2 - A simple and reliable capillary electrophoresis (CE) method with UV-vis detection is presented for the enantioselective separation and determination of vigabatrin enantiomers. Dehydroabietylisothiocyante (DHAIC), a novel chiral derivatizing reagent, was used for precolumn derivatization of vigabatrin enantiomers. Optimal separation was obtained with a running buffer consisting of 50 mM Na2HPO4 (pH 9.0), 17 mM sodium dodecyl sulfate (SDS) and 25% acetonitrile. The enantiomeric separation of vigabatrin derivatives was achieved within 25 min, and the resolution was found to be 2.1. Detection was followed by direct UV absorptiometric measurements at 202 nm. A calibration curve ranging from 0.3 to 6.0 microg/ml was shown to be linear, and the limit of detection was 0.15 microg/ml. The developed method has been applied to the determination of vigabatrin enantiomers spiked in human plasma, no interferences were found from endogenous amino acids. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16488672/Capillary_electrophoresis_enantioselective_separation_of_vigabatrin_enantiomers_by_precolumn_derivatization_with_dehydroabietylisothiocyante_and_UV_vis_detection_ DB - PRIME DP - Unbound Medicine ER -