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Detection of microsatellite instability in endometrial cancer: advantages of a panel of five mononucleotide repeats over the National Cancer Institute panel of markers.
Carcinogenesis. 2006 May; 27(5):951-5.C

Abstract

The aim of this study was to find the optimal set of microsatellite markers for diagnosis of the microsatellite instability (MSI) phenotype in endometrial cancers. We compared the sensitivity, specificity and ease of use of a reference panel of five markers originally recommended by the National Cancer Institute (NCI) for colorectal cancer and a panel of five quasi-monomorphic mononucleotide repeat markers (pentaplex PCR system). We used these panels for establishing the MSI status of a series of 80 sporadic endometrial adenocarcinomas by comparing the allelic profiles of the markers between tumor and matching germline DNA. Both panels detected the same subset of 21 out of 80 (26%) endometrial MSI carcinomas. However, in the MSI cases, the mean instability of the five mononucleotide repeats was 96.1% as compared with a mean instability of 69.8% for the three dinucleotide repeats of the NCI panel, indicating a superiority of mononucleotide repeats over dinucleotide repeats in detecting MSI. The fact that the two panels of markers detect the same set of MSI tumors is due to the presence of two mononucleotide repeats within the NCI panel. As demonstrated previously in gastric and colon MSI cases, the pentaplex PCR reaction using mononucleotide repeats is thus an easier and more sensitive method than the NCI panel, for the screening of MSI status in endometrial tumors.

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Authors+Show Affiliations

Wong YF
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T., Hong Kong, China and Inserm, U762, Paris, F-75010 France;, Univ Paris 6, Paris, F-75005 France.
Cheung TH
No affiliation info available
Lo KW
No affiliation info available
Yim SF
No affiliation info available
Chan LK
No affiliation info available
Buhard O
No affiliation info available
Duval A
No affiliation info available
Chung TK
No affiliation info available
Hamelin R
No affiliation info available

MeSH

Biomarkers, TumorDNADinucleotide RepeatsEndometrial NeoplasmsFemaleGenomic InstabilityHumansMicrosatellite RepeatsNational Institutes of Health (U.S.)PhenotypePolymerase Chain ReactionRepetitive Sequences, Nucleic AcidUnited States

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16490738

Citation

Wong, Yick Fu, et al. "Detection of Microsatellite Instability in Endometrial Cancer: Advantages of a Panel of Five Mononucleotide Repeats Over the National Cancer Institute Panel of Markers." Carcinogenesis, vol. 27, no. 5, 2006, pp. 951-5.
Wong YF, Cheung TH, Lo KW, et al. Detection of microsatellite instability in endometrial cancer: advantages of a panel of five mononucleotide repeats over the National Cancer Institute panel of markers. Carcinogenesis. 2006;27(5):951-5.
Wong, Y. F., Cheung, T. H., Lo, K. W., Yim, S. F., Chan, L. K., Buhard, O., Duval, A., Chung, T. K., & Hamelin, R. (2006). Detection of microsatellite instability in endometrial cancer: advantages of a panel of five mononucleotide repeats over the National Cancer Institute panel of markers. Carcinogenesis, 27(5), 951-5.
Wong YF, et al. Detection of Microsatellite Instability in Endometrial Cancer: Advantages of a Panel of Five Mononucleotide Repeats Over the National Cancer Institute Panel of Markers. Carcinogenesis. 2006;27(5):951-5. PubMed PMID: 16490738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of microsatellite instability in endometrial cancer: advantages of a panel of five mononucleotide repeats over the National Cancer Institute panel of markers. AU - Wong,Yick Fu, AU - Cheung,Tak Hong, AU - Lo,Keith Wing Kit, AU - Yim,So Fan, AU - Chan,Loucia Kit Yin, AU - Buhard,Olivier, AU - Duval,Alex, AU - Chung,Tony Kwok Hung, AU - Hamelin,Richard, Y1 - 2006/02/20/ PY - 2006/2/24/pubmed PY - 2006/8/1/medline PY - 2006/2/24/entrez SP - 951 EP - 5 JF - Carcinogenesis JO - Carcinogenesis VL - 27 IS - 5 N2 - The aim of this study was to find the optimal set of microsatellite markers for diagnosis of the microsatellite instability (MSI) phenotype in endometrial cancers. We compared the sensitivity, specificity and ease of use of a reference panel of five markers originally recommended by the National Cancer Institute (NCI) for colorectal cancer and a panel of five quasi-monomorphic mononucleotide repeat markers (pentaplex PCR system). We used these panels for establishing the MSI status of a series of 80 sporadic endometrial adenocarcinomas by comparing the allelic profiles of the markers between tumor and matching germline DNA. Both panels detected the same subset of 21 out of 80 (26%) endometrial MSI carcinomas. However, in the MSI cases, the mean instability of the five mononucleotide repeats was 96.1% as compared with a mean instability of 69.8% for the three dinucleotide repeats of the NCI panel, indicating a superiority of mononucleotide repeats over dinucleotide repeats in detecting MSI. The fact that the two panels of markers detect the same set of MSI tumors is due to the presence of two mononucleotide repeats within the NCI panel. As demonstrated previously in gastric and colon MSI cases, the pentaplex PCR reaction using mononucleotide repeats is thus an easier and more sensitive method than the NCI panel, for the screening of MSI status in endometrial tumors. SN - 0143-3334 UR - https://www.unboundmedicine.com/medline/citation/16490738/Detection_of_microsatellite_instability_in_endometrial_cancer:_advantages_of_a_panel_of_five_mononucleotide_repeats_over_the_National_Cancer_Institute_panel_of_markers_ L2 - https://academic.oup.com/carcin/article-lookup/doi/10.1093/carcin/bgi333 DB - PRIME DP - Unbound Medicine ER -