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Protection against heterologous Burkholderia pseudomallei strains by dendritic cell immunization.
Infect Immun. 2006 Mar; 74(3):1706-11.II

Abstract

Burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium which can cause either chronic infections or acute lethal sepsis in infected individuals. The disease is endemic in Southeast Asia and northern Australia, but little is known about the mechanisms of protective immunity to the bacterium. In this study, we have developed a procedure to utilize dendritic cells in combination with CpG oligodeoxynucleotides as a vaccine delivery vector to induce protective immune responses to various strains of B. pseudomallei. Our results show that strong cell-mediated immune responses were generated, while antibody responses, although low, were detectable. Upon virulent challenge with B. pseudomallei strain K96243, NCTC 4845, or 576, animals immunized with dendritic cells that were pulsed with heat-killed K96243 and matured in the presence of CpG 1826 showed significant levels of protection. These results show that a vaccine strategy that actively targets dendritic cells can evoke protective immune responses.

Authors+Show Affiliations

Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, United Kingdom. SJElvin@dstl.gov.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16495542

Citation

Elvin, Stephen J., et al. "Protection Against Heterologous Burkholderia Pseudomallei Strains By Dendritic Cell Immunization." Infection and Immunity, vol. 74, no. 3, 2006, pp. 1706-11.
Elvin SJ, Healey GD, Westwood A, et al. Protection against heterologous Burkholderia pseudomallei strains by dendritic cell immunization. Infect Immun. 2006;74(3):1706-11.
Elvin, S. J., Healey, G. D., Westwood, A., Knight, S. C., Eyles, J. E., & Williamson, E. D. (2006). Protection against heterologous Burkholderia pseudomallei strains by dendritic cell immunization. Infection and Immunity, 74(3), 1706-11.
Elvin SJ, et al. Protection Against Heterologous Burkholderia Pseudomallei Strains By Dendritic Cell Immunization. Infect Immun. 2006;74(3):1706-11. PubMed PMID: 16495542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against heterologous Burkholderia pseudomallei strains by dendritic cell immunization. AU - Elvin,Stephen J, AU - Healey,Gareth D, AU - Westwood,Angie, AU - Knight,Stella C, AU - Eyles,James E, AU - Williamson,E Diane, PY - 2006/2/24/pubmed PY - 2006/6/6/medline PY - 2006/2/24/entrez SP - 1706 EP - 11 JF - Infection and immunity JO - Infect Immun VL - 74 IS - 3 N2 - Burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium which can cause either chronic infections or acute lethal sepsis in infected individuals. The disease is endemic in Southeast Asia and northern Australia, but little is known about the mechanisms of protective immunity to the bacterium. In this study, we have developed a procedure to utilize dendritic cells in combination with CpG oligodeoxynucleotides as a vaccine delivery vector to induce protective immune responses to various strains of B. pseudomallei. Our results show that strong cell-mediated immune responses were generated, while antibody responses, although low, were detectable. Upon virulent challenge with B. pseudomallei strain K96243, NCTC 4845, or 576, animals immunized with dendritic cells that were pulsed with heat-killed K96243 and matured in the presence of CpG 1826 showed significant levels of protection. These results show that a vaccine strategy that actively targets dendritic cells can evoke protective immune responses. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/16495542/Protection_against_heterologous_Burkholderia_pseudomallei_strains_by_dendritic_cell_immunization_ L2 - https://journals.asm.org/doi/10.1128/IAI.74.3.1706-1711.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -