Tags

Type your tag names separated by a space and hit enter

Generation of an Fgf9 conditional null allele.
Genesis. 2006 Mar; 44(3):150-4.G

Abstract

The fibroblast growth factor (FGF) family consists of 22 widely expressed regulatory polypeptides and controls a broad spectrum of cellular processes. Accumulating data show that FGF9 plays important roles both in embryogenesis and in adult tissue homeostasis. Ablation of Fgf9 alleles leads to lethality at the neonatal stage mainly due to malformations of the lung, as well as causing male-to-female sex reversal. To circumvent the neonatal lethality resulting from disruption of the Fgf9 gene, which hinders further characterization of the role of FGF9 in adult tissue function and homeostasis, we generated an Fgf9 conditional null allele for spatiotemporal- and tissue-specific disruption of Fgf9. Using gene targeting in mouse embryonic stem (ES) cells, we introduced two loxP sites flanking exon 1 in the Fgf9 allele, which encodes 93 amino acid residues at the N-terminal of FGF9. Our results indicate that the Fgf9 conditional null allele is a true conditional null that encodes wildtype activity and reverts to a null allele after recombination mediated by the Cre recombinase.

Authors+Show Affiliations

Center for Cancer Biology and Nutrition, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030-3303, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16496342

Citation

Lin, Yongshun, et al. "Generation of an Fgf9 Conditional Null Allele." Genesis (New York, N.Y. : 2000), vol. 44, no. 3, 2006, pp. 150-4.
Lin Y, Liu G, Wang F. Generation of an Fgf9 conditional null allele. Genesis. 2006;44(3):150-4.
Lin, Y., Liu, G., & Wang, F. (2006). Generation of an Fgf9 conditional null allele. Genesis (New York, N.Y. : 2000), 44(3), 150-4.
Lin Y, Liu G, Wang F. Generation of an Fgf9 Conditional Null Allele. Genesis. 2006;44(3):150-4. PubMed PMID: 16496342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of an Fgf9 conditional null allele. AU - Lin,Yongshun, AU - Liu,Guoqin, AU - Wang,Fen, PY - 2006/2/24/pubmed PY - 2006/6/21/medline PY - 2006/2/24/entrez SP - 150 EP - 4 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 44 IS - 3 N2 - The fibroblast growth factor (FGF) family consists of 22 widely expressed regulatory polypeptides and controls a broad spectrum of cellular processes. Accumulating data show that FGF9 plays important roles both in embryogenesis and in adult tissue homeostasis. Ablation of Fgf9 alleles leads to lethality at the neonatal stage mainly due to malformations of the lung, as well as causing male-to-female sex reversal. To circumvent the neonatal lethality resulting from disruption of the Fgf9 gene, which hinders further characterization of the role of FGF9 in adult tissue function and homeostasis, we generated an Fgf9 conditional null allele for spatiotemporal- and tissue-specific disruption of Fgf9. Using gene targeting in mouse embryonic stem (ES) cells, we introduced two loxP sites flanking exon 1 in the Fgf9 allele, which encodes 93 amino acid residues at the N-terminal of FGF9. Our results indicate that the Fgf9 conditional null allele is a true conditional null that encodes wildtype activity and reverts to a null allele after recombination mediated by the Cre recombinase. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/16496342/Generation_of_an_Fgf9_conditional_null_allele_ L2 - https://doi.org/10.1002/gene.20194 DB - PRIME DP - Unbound Medicine ER -