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Conditional (loxP-flanked) allele for the gene encoding the retinoic acid-synthesizing enzyme retinaldehyde dehydrogenase 2 (RALDH2).
Genesis. 2006 Mar; 44(3):155-8.G

Abstract

Retinoic acid, the active vitamin A derivative, has pleiotropic functions during vertebrate development and postnatal life. Retinaldehyde dehydrogenase 2 (RALDH2) acts as the main retinoic acid-synthesizing enzyme during development. Mouse Raldh2 germline null mutants are early embryonic lethal and exhibit complex abnormalities that include defective heart looping morphogenesis. To investigate later functions of this enzyme, we have engineered a "floxed" (loxP-flanked) allele allowing Cre-mediated somatic gene inactivations. Mice heterozygous or homozygous for the floxed Raldh2 allele are viable and fertile. We tested whether the novel Raldh2 allele behaves as a null mutation after Cre-mediated in vivo excision by crossing the conditional mutants with CMV-Cre transgenic mice. An embryonic lethal phenotype indistinguishable from that of germline mutants was obtained. The conditional allele described herein is a genetic tool for studying tissue-specific, RALDH2-dependent functions of retinoic acid during development and in adult life.

Authors+Show Affiliations

Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104 du CNRS, U. 596 de l'INSERM, Université Louis Pasteur, CU de Strasbourg, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16496350

Citation

Vermot, Julien, et al. "Conditional (loxP-flanked) Allele for the Gene Encoding the Retinoic Acid-synthesizing Enzyme Retinaldehyde Dehydrogenase 2 (RALDH2)." Genesis (New York, N.Y. : 2000), vol. 44, no. 3, 2006, pp. 155-8.
Vermot J, Garnier JM, Dierich A, et al. Conditional (loxP-flanked) allele for the gene encoding the retinoic acid-synthesizing enzyme retinaldehyde dehydrogenase 2 (RALDH2). Genesis. 2006;44(3):155-8.
Vermot, J., Garnier, J. M., Dierich, A., Niederreither, K., Harvey, R. P., Chambon, P., & Dollé, P. (2006). Conditional (loxP-flanked) allele for the gene encoding the retinoic acid-synthesizing enzyme retinaldehyde dehydrogenase 2 (RALDH2). Genesis (New York, N.Y. : 2000), 44(3), 155-8.
Vermot J, et al. Conditional (loxP-flanked) Allele for the Gene Encoding the Retinoic Acid-synthesizing Enzyme Retinaldehyde Dehydrogenase 2 (RALDH2). Genesis. 2006;44(3):155-8. PubMed PMID: 16496350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conditional (loxP-flanked) allele for the gene encoding the retinoic acid-synthesizing enzyme retinaldehyde dehydrogenase 2 (RALDH2). AU - Vermot,Julien, AU - Garnier,Jean-Marie, AU - Dierich,Andrée, AU - Niederreither,Karen, AU - Harvey,Richard P, AU - Chambon,Pierre, AU - Dollé,Pascal, PY - 2006/2/24/pubmed PY - 2006/6/21/medline PY - 2006/2/24/entrez SP - 155 EP - 8 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 44 IS - 3 N2 - Retinoic acid, the active vitamin A derivative, has pleiotropic functions during vertebrate development and postnatal life. Retinaldehyde dehydrogenase 2 (RALDH2) acts as the main retinoic acid-synthesizing enzyme during development. Mouse Raldh2 germline null mutants are early embryonic lethal and exhibit complex abnormalities that include defective heart looping morphogenesis. To investigate later functions of this enzyme, we have engineered a "floxed" (loxP-flanked) allele allowing Cre-mediated somatic gene inactivations. Mice heterozygous or homozygous for the floxed Raldh2 allele are viable and fertile. We tested whether the novel Raldh2 allele behaves as a null mutation after Cre-mediated in vivo excision by crossing the conditional mutants with CMV-Cre transgenic mice. An embryonic lethal phenotype indistinguishable from that of germline mutants was obtained. The conditional allele described herein is a genetic tool for studying tissue-specific, RALDH2-dependent functions of retinoic acid during development and in adult life. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/16496350/Conditional__loxP_flanked__allele_for_the_gene_encoding_the_retinoic_acid_synthesizing_enzyme_retinaldehyde_dehydrogenase_2__RALDH2__ L2 - https://doi.org/10.1002/gene.20195 DB - PRIME DP - Unbound Medicine ER -