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Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells.
Yao Xue Xue Bao 2005; 40(12):1099-104YX

Abstract

AIM

To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel.

METHODS

Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs).

RESULTS

Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively.

CONCLUSION

Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.

Authors+Show Affiliations

Applied Research Center for Genomics Technology, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16496673

Citation

Wang, Jin, et al. "Differential Gene Expression Profiles in Paclitaxel-induced Cell Cycle Arrest and Apoptosis in Human Breast Cancer MCF-7 Cells." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 40, no. 12, 2005, pp. 1099-104.
Wang J, He FT, Tzang CH, et al. Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells. Yao Xue Xue Bao. 2005;40(12):1099-104.
Wang, J., He, F. T., Tzang, C. H., Fong, W. F., Xiao, P. G., Han, R., & Yang, M. S. (2005). Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 40(12), pp. 1099-104.
Wang J, et al. Differential Gene Expression Profiles in Paclitaxel-induced Cell Cycle Arrest and Apoptosis in Human Breast Cancer MCF-7 Cells. Yao Xue Xue Bao. 2005;40(12):1099-104. PubMed PMID: 16496673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells. AU - Wang,Jin, AU - He,Fang-ting, AU - Tzang,Chi-hung, AU - Fong,Wang-fan, AU - Xiao,Pei-gen, AU - Han,Rui, AU - Yang,Meng-su, PY - 2006/2/25/pubmed PY - 2007/8/7/medline PY - 2006/2/25/entrez SP - 1099 EP - 104 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 40 IS - 12 N2 - AIM: To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. METHODS: Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs). RESULTS: Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively. CONCLUSION: Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/16496673/Differential_gene_expression_profiles_in_paclitaxel_induced_cell_cycle_arrest_and_apoptosis_in_human_breast_cancer_MCF_7_cells_ L2 - http://www.diseaseinfosearch.org/result/960 DB - PRIME DP - Unbound Medicine ER -