Tags

Type your tag names separated by a space and hit enter

Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma.
Biol Chem. 2006 Feb; 387(2):173-8.BC

Abstract

Blood coagulation factor XII (FXII, Hageman factor) is a plasma serine protease which is autoactivated following contact with negatively charged surfaces in a reaction involving plasma kallikrein and high-molecular-weight kininogen (contact phase activation). Active FXII has the ability to initiate blood clotting via the intrinsic pathway of coagulation and inflammatory reactions via the kallikrein-kinin system. Here we have determined FXII-mediated bradykinin formation and clotting in plasma. Western blotting analysis with specific antibodies against various parts of the contact factors revealed that limited activation of FXII is sufficient to promote plasma kallikrein activation, resulting in the conversion of high-molecular-weight kininogen and bradykinin generation. The presence of platelets significantly promoted FXII-initiated bradykinin formation. Similarly, in vitro clotting assays revealed that platelets critically promoted FXII-driven thrombin and fibrin formation. In summary, our data suggest that FXII-initiated protease cascades may proceed on platelet surfaces, with implications for inflammation and clotting.

Authors+Show Affiliations

1. Institute of Clinical Biochemistry and Pathobiochemistry, Josef-Schneider-Strasse 2, University of Würzburg, D-97080 Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16497149

Citation

Johne, Julia, et al. "Platelets Promote Coagulation Factor XII-mediated Proteolytic Cascade Systems in Plasma." Biological Chemistry, vol. 387, no. 2, 2006, pp. 173-8.
Johne J, Blume C, Benz PM, et al. Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma. Biol Chem. 2006;387(2):173-8.
Johne, J., Blume, C., Benz, P. M., Pozgajová, M., Ullrich, M., Schuh, K., Nieswandt, B., Walter, U., & Renné, T. (2006). Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma. Biological Chemistry, 387(2), 173-8.
Johne J, et al. Platelets Promote Coagulation Factor XII-mediated Proteolytic Cascade Systems in Plasma. Biol Chem. 2006;387(2):173-8. PubMed PMID: 16497149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma. AU - Johne,Julia, AU - Blume,Constanze, AU - Benz,Peter M, AU - Pozgajová,Miroslava, AU - Ullrich,Melanie, AU - Schuh,Kai, AU - Nieswandt,Bernhard, AU - Walter,Ulrich, AU - Renné,Thomas, PY - 2006/2/25/pubmed PY - 2006/7/11/medline PY - 2006/2/25/entrez SP - 173 EP - 8 JF - Biological chemistry JO - Biol Chem VL - 387 IS - 2 N2 - Blood coagulation factor XII (FXII, Hageman factor) is a plasma serine protease which is autoactivated following contact with negatively charged surfaces in a reaction involving plasma kallikrein and high-molecular-weight kininogen (contact phase activation). Active FXII has the ability to initiate blood clotting via the intrinsic pathway of coagulation and inflammatory reactions via the kallikrein-kinin system. Here we have determined FXII-mediated bradykinin formation and clotting in plasma. Western blotting analysis with specific antibodies against various parts of the contact factors revealed that limited activation of FXII is sufficient to promote plasma kallikrein activation, resulting in the conversion of high-molecular-weight kininogen and bradykinin generation. The presence of platelets significantly promoted FXII-initiated bradykinin formation. Similarly, in vitro clotting assays revealed that platelets critically promoted FXII-driven thrombin and fibrin formation. In summary, our data suggest that FXII-initiated protease cascades may proceed on platelet surfaces, with implications for inflammation and clotting. SN - 1431-6730 UR - https://www.unboundmedicine.com/medline/citation/16497149/Platelets_promote_coagulation_factor_XII_mediated_proteolytic_cascade_systems_in_plasma_ L2 - https://www.degruyter.com/document/doi/10.1515/BC.2006.023 DB - PRIME DP - Unbound Medicine ER -