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Spray-dried milk supplemented with alpha-linolenic acid or eicosapentaenoic acid and docosahexaenoic acid decreases HMG Co A reductase activity and increases biliary secretion of lipids in rats.
Steroids. 2006 May; 71(5):409-15.S

Abstract

In our earlier study, we have shown that rats fed spray-dried milk containing alpha-linolenic acid (LNA 18:3 n-3) or eicosapentaenoic acid (EPA 20:5 n-3) and docosahexaenoic acid (DHA 22:6 n-3) had significantly lower amounts of serum and liver cholesterol. To evaluate the mechanism for hypocholesterolemic effect of n-3 fatty acids containing milk formulation, we fed male Wistar rats with spray-dried milk containing linseed oil (LSO) (source of LNA) or fish oil (FO) (source of EPA+DHA) for 8 weeks. Feeding n-3 fatty acid containing milk formulation lowered the hepatic 3-hydroxy-methylglutaryl coenzyme A (HMG Co A) activity by 17-22% compared to rats given control diet devoid of n-3 fatty acids. The cholesterol level in liver microsomes was found to be decreased by 16% and 20%, respectively, in LSO and FO containing formulation fed rats. The bile flow was enhanced to an extent of 19-23% in experimental groups compared to control animals. The biliary cholesterol and phospholipid secretion was increased to an extent of 49-55% and 140-146%, respectively, in rats fed n-3 fatty acid containing formulation. The increase in the total bile acids secretion in bile was mainly reflected on an increase in the levels of taurine conjugated bile acids. These results indicated that n-3 fatty acid containing spray-dried milk formulation would bring about the hypocholesterolemic effect by lowering HMG Co A reductase activity in liver and by increasing the secretion of bile constituents.

Authors+Show Affiliations

Department of Lipid Science and Traditional Foods, Central Food Technological Research Institute, Mysore, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16499938

Citation

Ramaprasad, Talahalli R., et al. "Spray-dried Milk Supplemented With Alpha-linolenic Acid or Eicosapentaenoic Acid and Docosahexaenoic Acid Decreases HMG Co a Reductase Activity and Increases Biliary Secretion of Lipids in Rats." Steroids, vol. 71, no. 5, 2006, pp. 409-15.
Ramaprasad TR, Srinivasan K, Baskaran V, et al. Spray-dried milk supplemented with alpha-linolenic acid or eicosapentaenoic acid and docosahexaenoic acid decreases HMG Co A reductase activity and increases biliary secretion of lipids in rats. Steroids. 2006;71(5):409-15.
Ramaprasad, T. R., Srinivasan, K., Baskaran, V., Sambaiah, K., & Lokesh, B. R. (2006). Spray-dried milk supplemented with alpha-linolenic acid or eicosapentaenoic acid and docosahexaenoic acid decreases HMG Co A reductase activity and increases biliary secretion of lipids in rats. Steroids, 71(5), 409-15.
Ramaprasad TR, et al. Spray-dried Milk Supplemented With Alpha-linolenic Acid or Eicosapentaenoic Acid and Docosahexaenoic Acid Decreases HMG Co a Reductase Activity and Increases Biliary Secretion of Lipids in Rats. Steroids. 2006;71(5):409-15. PubMed PMID: 16499938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spray-dried milk supplemented with alpha-linolenic acid or eicosapentaenoic acid and docosahexaenoic acid decreases HMG Co A reductase activity and increases biliary secretion of lipids in rats. AU - Ramaprasad,Talahalli R, AU - Srinivasan,Krishnapura, AU - Baskaran,Vallikannan, AU - Sambaiah,Kari, AU - Lokesh,Belur R, Y1 - 2006/02/24/ PY - 2005/05/10/received PY - 2005/12/19/revised PY - 2006/01/04/accepted PY - 2006/2/28/pubmed PY - 2006/7/18/medline PY - 2006/2/28/entrez SP - 409 EP - 15 JF - Steroids JO - Steroids VL - 71 IS - 5 N2 - In our earlier study, we have shown that rats fed spray-dried milk containing alpha-linolenic acid (LNA 18:3 n-3) or eicosapentaenoic acid (EPA 20:5 n-3) and docosahexaenoic acid (DHA 22:6 n-3) had significantly lower amounts of serum and liver cholesterol. To evaluate the mechanism for hypocholesterolemic effect of n-3 fatty acids containing milk formulation, we fed male Wistar rats with spray-dried milk containing linseed oil (LSO) (source of LNA) or fish oil (FO) (source of EPA+DHA) for 8 weeks. Feeding n-3 fatty acid containing milk formulation lowered the hepatic 3-hydroxy-methylglutaryl coenzyme A (HMG Co A) activity by 17-22% compared to rats given control diet devoid of n-3 fatty acids. The cholesterol level in liver microsomes was found to be decreased by 16% and 20%, respectively, in LSO and FO containing formulation fed rats. The bile flow was enhanced to an extent of 19-23% in experimental groups compared to control animals. The biliary cholesterol and phospholipid secretion was increased to an extent of 49-55% and 140-146%, respectively, in rats fed n-3 fatty acid containing formulation. The increase in the total bile acids secretion in bile was mainly reflected on an increase in the levels of taurine conjugated bile acids. These results indicated that n-3 fatty acid containing spray-dried milk formulation would bring about the hypocholesterolemic effect by lowering HMG Co A reductase activity in liver and by increasing the secretion of bile constituents. SN - 0039-128X UR - https://www.unboundmedicine.com/medline/citation/16499938/Spray_dried_milk_supplemented_with_alpha_linolenic_acid_or_eicosapentaenoic_acid_and_docosahexaenoic_acid_decreases_HMG_Co_A_reductase_activity_and_increases_biliary_secretion_of_lipids_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-128X(06)00017-1 DB - PRIME DP - Unbound Medicine ER -