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Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 03; 833(2):129-34.JC

Abstract

The capillary electrophoresis (CE) system with optical fiber light-emitting diode (optical fiber LED) induced fluorescence detector was developed for the analysis of the excitatory amino acids (EAAs) tagged with naphthalene-2,3-dicarboxaldehyde (NDA). The separation of EAAs was carried out in an uncoated fused-silica capillary (50 cm x 75 microm i.d.) with a buffer of 10 mM borate at pH 9.3 and an applied voltage of 20 kV. High sensitivity was obtained by the use of optical fiber LED induced fluorescence detector with a violet LED as the excitation light source. The limits of detection (S/N = 3) for glutamic acid (Glu) and aspartic acid (Asp) were 2.1 x 10(-8) and 2.3 x 10(-8) M, respectively. The detection approach was successfully applied to the analysis of Glu and Asp in biological fluids including human serum, rabbit serum and human cerebrospinal fluid (CSF) with satisfactory results.

Authors+Show Affiliations

Wang C
College of Chemistry, Sichuan University, Chengdu 610065, China.
Zhao S
No affiliation info available
Yuan H
No affiliation info available
Xiao D
No affiliation info available

MeSH

AnimalsElectrophoresis, CapillaryExcitatory Amino AcidsHumansOptics and PhotonicsRabbitsSensitivity and SpecificitySpectrometry, FluorescenceSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16500157

Citation

Wang, Chunling, et al. "Determination of Excitatory Amino Acids in Biological Fluids By Capillary Electrophoresis With Optical Fiber Light-emitting Diode Induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 833, no. 2, 2006, pp. 129-34.
Wang C, Zhao S, Yuan H, et al. Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;833(2):129-34.
Wang, C., Zhao, S., Yuan, H., & Xiao, D. (2006). Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 833(2), 129-34.
Wang C, et al. Determination of Excitatory Amino Acids in Biological Fluids By Capillary Electrophoresis With Optical Fiber Light-emitting Diode Induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 3;833(2):129-34. PubMed PMID: 16500157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection. AU - Wang,Chunling, AU - Zhao,Shulin, AU - Yuan,Hongyan, AU - Xiao,Dan, Y1 - 2006/02/24/ PY - 2005/10/23/received PY - 2005/12/28/revised PY - 2006/01/12/accepted PY - 2006/2/28/pubmed PY - 2006/7/18/medline PY - 2006/2/28/entrez SP - 129 EP - 34 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 833 IS - 2 N2 - The capillary electrophoresis (CE) system with optical fiber light-emitting diode (optical fiber LED) induced fluorescence detector was developed for the analysis of the excitatory amino acids (EAAs) tagged with naphthalene-2,3-dicarboxaldehyde (NDA). The separation of EAAs was carried out in an uncoated fused-silica capillary (50 cm x 75 microm i.d.) with a buffer of 10 mM borate at pH 9.3 and an applied voltage of 20 kV. High sensitivity was obtained by the use of optical fiber LED induced fluorescence detector with a violet LED as the excitation light source. The limits of detection (S/N = 3) for glutamic acid (Glu) and aspartic acid (Asp) were 2.1 x 10(-8) and 2.3 x 10(-8) M, respectively. The detection approach was successfully applied to the analysis of Glu and Asp in biological fluids including human serum, rabbit serum and human cerebrospinal fluid (CSF) with satisfactory results. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16500157/Determination_of_excitatory_amino_acids_in_biological_fluids_by_capillary_electrophoresis_with_optical_fiber_light_emitting_diode_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -