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Two-week feeding of difructose anhydride III enhances calcium absorptive activity with epithelial cell proliferation in isolated rat cecal mucosa.
Nutrition. 2006 Mar; 22(3):312-20.N

Abstract

OBJECTIVE

Ingestion of difructose anhydride III (DFAIII) enhances calcium (Ca) absorption in rats. The present study investigated the mechanism involved in increased Ca transport by DFAIII ingestion. The short-term and long-term effects of DFAIII feeding on Ca transport were determined by using isolated epithelium from the small and large intestine in rats.

METHODS

Male Sprague-Dawley rats were fed an 8% cellulose or 5% cellulose plus 3% DFAIII diet for 14 d. Net epithelial Ca transport in the small intestine, cecum, and colon was compared between the two diet groups by using an Ussing chamber. The contents and epithelial tissues in the cecum were analyzed.

RESULTS

There were no differences in basal and luminal DFAIII-induced Ca transport in the isolated small intestinal and colonic mucosa between the two diet groups. Basal and lumen DFAIII-induced Ca transport in the cecum in the DFAIII-fed group was higher than that in the control group. A decrease in pH and an increase in Ca pools, short-chain fatty acids, or organic acids in the cecal contents and in the depth and number of cells in crypts in cecal tissue were observed.

CONCLUSIONS

The increase in Ca transport involved two mechanisms: the presence of DFAIII in the small intestine directly affected the epithelial tissue and caused increased Ca absorption as a short-term effect, and the degradation of DFAIII by microbial fermentation produced short-chain fatty acids and subsequently enhanced Ca absorption in the large intestine as a long-term effect.

Authors+Show Affiliations

Mineo H
Northern Advancement Center for Science and Technology, Colabo-Hokkaido, Sapporo, Japan.
Amano M
No affiliation info available
Minaminida K
No affiliation info available
Chiji H
No affiliation info available
Shigematsu N
No affiliation info available
Tomita F
No affiliation info available
Hara H
No affiliation info available

MeSH

AnimalsCalciumCecumCell DivisionCell Membrane PermeabilityCelluloseColonDisaccharidesEpithelial CellsFatty Acids, VolatileFermentationHydrogen-Ion ConcentrationIntestinal AbsorptionIntestinal MucosaMaleRandom AllocationRatsRats, Sprague-Dawley

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16500557

Citation

Mineo, Hitoshi, et al. "Two-week Feeding of Difructose Anhydride III Enhances Calcium Absorptive Activity With Epithelial Cell Proliferation in Isolated Rat Cecal Mucosa." Nutrition (Burbank, Los Angeles County, Calif.), vol. 22, no. 3, 2006, pp. 312-20.
Mineo H, Amano M, Minaminida K, et al. Two-week feeding of difructose anhydride III enhances calcium absorptive activity with epithelial cell proliferation in isolated rat cecal mucosa. Nutrition. 2006;22(3):312-20.
Mineo, H., Amano, M., Minaminida, K., Chiji, H., Shigematsu, N., Tomita, F., & Hara, H. (2006). Two-week feeding of difructose anhydride III enhances calcium absorptive activity with epithelial cell proliferation in isolated rat cecal mucosa. Nutrition (Burbank, Los Angeles County, Calif.), 22(3), 312-20.
Mineo H, et al. Two-week Feeding of Difructose Anhydride III Enhances Calcium Absorptive Activity With Epithelial Cell Proliferation in Isolated Rat Cecal Mucosa. Nutrition. 2006;22(3):312-20. PubMed PMID: 16500557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-week feeding of difructose anhydride III enhances calcium absorptive activity with epithelial cell proliferation in isolated rat cecal mucosa. AU - Mineo,Hitoshi, AU - Amano,Midori, AU - Minaminida,Kimiko, AU - Chiji,Hideyuki, AU - Shigematsu,Norihiro, AU - Tomita,Fusao, AU - Hara,Hiroshi, PY - 2005/03/15/received PY - 2005/06/02/accepted PY - 2006/2/28/pubmed PY - 2006/8/1/medline PY - 2006/2/28/entrez SP - 312 EP - 20 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 22 IS - 3 N2 - OBJECTIVE: Ingestion of difructose anhydride III (DFAIII) enhances calcium (Ca) absorption in rats. The present study investigated the mechanism involved in increased Ca transport by DFAIII ingestion. The short-term and long-term effects of DFAIII feeding on Ca transport were determined by using isolated epithelium from the small and large intestine in rats. METHODS: Male Sprague-Dawley rats were fed an 8% cellulose or 5% cellulose plus 3% DFAIII diet for 14 d. Net epithelial Ca transport in the small intestine, cecum, and colon was compared between the two diet groups by using an Ussing chamber. The contents and epithelial tissues in the cecum were analyzed. RESULTS: There were no differences in basal and luminal DFAIII-induced Ca transport in the isolated small intestinal and colonic mucosa between the two diet groups. Basal and lumen DFAIII-induced Ca transport in the cecum in the DFAIII-fed group was higher than that in the control group. A decrease in pH and an increase in Ca pools, short-chain fatty acids, or organic acids in the cecal contents and in the depth and number of cells in crypts in cecal tissue were observed. CONCLUSIONS: The increase in Ca transport involved two mechanisms: the presence of DFAIII in the small intestine directly affected the epithelial tissue and caused increased Ca absorption as a short-term effect, and the degradation of DFAIII by microbial fermentation produced short-chain fatty acids and subsequently enhanced Ca absorption in the large intestine as a long-term effect. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/16500557/Two_week_feeding_of_difructose_anhydride_III_enhances_calcium_absorptive_activity_with_epithelial_cell_proliferation_in_isolated_rat_cecal_mucosa_ DB - PRIME DP - Unbound Medicine ER -