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Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast.
Planta. 2006 Aug; 224(3):545-55.P

Abstract

Nitric oxide (NO), an endogenous signaling molecule in animals and plants, mediates responses to abiotic and biotic stresses. Our previous work demonstrated that 100 microM sodium nitroprusside (SNP, an NO donor) treatment of maize seedlings increased K(+) accumulation in roots, leaves and sheathes, while decreasing Na(+) accumulation (Zhang et al. in J Plant Physiol Mol Biol 30:455-459, 2004b). Here we investigate how NO regulates Na(+), K(+) ion homeostasis in maize. Pre-treatment with 100 muM SNP for 2 days improved later growth of maize plants under 100 mM NaCl stress, as indicated by increased dry matter accumulation, increased chlorophyll content, and decreased membrane leakage from leaf cells. An NO scavenger, methylene blue (MB-1), blocked the effect of SNP. These results indicated that SNP-derived NO enhanced maize tolerance to salt stress. Further analysis showed that NaCl induced a transient increase in the NO level in maize leaves. Both NO and NaCl treatment stimulated vacuolar H(+)-ATPase and H(+)-PPase activities, resulting in increased H(+)-translocation and Na(+)/H(+) exchange. NaCl-induced H(+)-ATPase and H(+)-PPase activities were diminished by MB-1. 1-Butanol, an inhibitor of phosphatidic acid (PA) production by phospholipase D (PLD), reduced NaCl- and NO-induced H(+)-ATPase activation. In contrast, applied PA stimulated H(+)-ATPase activity. These results suggest that NO acts as a signal molecule in the NaCl response by increasing the activities of vacuolar H(+)-ATPase and H(+)-PPase, which provide the driving force for Na(+)/H(+) exchange. PLD and PA play an important role in this process.

Authors+Show Affiliations

Zhang Y
College of Life Science and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Wang L
No affiliation info available
Liu Y
No affiliation info available
Zhang Q
No affiliation info available
Wei Q
No affiliation info available
Zhang W
No affiliation info available

MeSH

Cell MembraneFatty Acids, NonesterifiedHomeostasisNitric OxideNitric Oxide DonorsNitroprussidePhosphatidic AcidsPhospholipase DPlant LeavesPotassiumProton PumpsSeedlingsSodiumSodium ChlorideSodium-Hydrogen ExchangersWaterZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16501990

Citation

Zhang, Yanyan, et al. "Nitric Oxide Enhances Salt Tolerance in Maize Seedlings Through Increasing Activities of Proton-pump and Na+/H+ Antiport in the Tonoplast." Planta, vol. 224, no. 3, 2006, pp. 545-55.
Zhang Y, Wang L, Liu Y, et al. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta. 2006;224(3):545-55.
Zhang, Y., Wang, L., Liu, Y., Zhang, Q., Wei, Q., & Zhang, W. (2006). Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta, 224(3), 545-55.
Zhang Y, et al. Nitric Oxide Enhances Salt Tolerance in Maize Seedlings Through Increasing Activities of Proton-pump and Na+/H+ Antiport in the Tonoplast. Planta. 2006;224(3):545-55. PubMed PMID: 16501990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. AU - Zhang,Yanyan, AU - Wang,Liling, AU - Liu,Youliang, AU - Zhang,Qun, AU - Wei,Qiuping, AU - Zhang,Wenhua, Y1 - 2006/02/25/ PY - 2005/11/10/received PY - 2006/02/08/accepted PY - 2006/2/28/pubmed PY - 2006/11/7/medline PY - 2006/2/28/entrez SP - 545 EP - 55 JF - Planta JO - Planta VL - 224 IS - 3 N2 - Nitric oxide (NO), an endogenous signaling molecule in animals and plants, mediates responses to abiotic and biotic stresses. Our previous work demonstrated that 100 microM sodium nitroprusside (SNP, an NO donor) treatment of maize seedlings increased K(+) accumulation in roots, leaves and sheathes, while decreasing Na(+) accumulation (Zhang et al. in J Plant Physiol Mol Biol 30:455-459, 2004b). Here we investigate how NO regulates Na(+), K(+) ion homeostasis in maize. Pre-treatment with 100 muM SNP for 2 days improved later growth of maize plants under 100 mM NaCl stress, as indicated by increased dry matter accumulation, increased chlorophyll content, and decreased membrane leakage from leaf cells. An NO scavenger, methylene blue (MB-1), blocked the effect of SNP. These results indicated that SNP-derived NO enhanced maize tolerance to salt stress. Further analysis showed that NaCl induced a transient increase in the NO level in maize leaves. Both NO and NaCl treatment stimulated vacuolar H(+)-ATPase and H(+)-PPase activities, resulting in increased H(+)-translocation and Na(+)/H(+) exchange. NaCl-induced H(+)-ATPase and H(+)-PPase activities were diminished by MB-1. 1-Butanol, an inhibitor of phosphatidic acid (PA) production by phospholipase D (PLD), reduced NaCl- and NO-induced H(+)-ATPase activation. In contrast, applied PA stimulated H(+)-ATPase activity. These results suggest that NO acts as a signal molecule in the NaCl response by increasing the activities of vacuolar H(+)-ATPase and H(+)-PPase, which provide the driving force for Na(+)/H(+) exchange. PLD and PA play an important role in this process. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/16501990/Nitric_oxide_enhances_salt_tolerance_in_maize_seedlings_through_increasing_activities_of_proton_pump_and_Na+/H+_antiport_in_the_tonoplast_ DB - PRIME DP - Unbound Medicine ER -