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Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass.
Eur J Cardiothorac Surg. 2006 Apr; 29(4):496-500.EJ

Abstract

OBJECTIVE

We compared profiles of the numbers of circulating endothelial cells (CEC) and the apoptosis-inducing capacity of serum samples on human endothelial cells (hEC) in on-pump and off-pump coronary artery bypass grafting (CABG) patients.

METHODS

Blood samples from 30 patients undergoing CABG (randomly assigned to two groups: 15 patients off-pump and 15 on-pump (cardiopulmonary bypass, CPB)) were collected after induction of anesthesia (preoperatively), at weaning from CPB/end of bypass grafting (0 h), and 1, 6, 12, 24, and 48 h afterwards. CEC were isolated with immunomagnetic anti-CD146-coated Dynabeads, and counted in a Nageotte chamber. The apoptosis-inducing activity of serum samples on hEC was examined by a tissue culture assay system. Apoptotic and normal cells were identified using phase contrast/fluorescence microscopy after DNA dye staining.

RESULTS

CEC numbers and proportions of apoptotic hEC were significantly elevated during and after surgery in both groups (p<0.01). Compared with the on-pump group, CEC and proportions of apoptotic hEC were significantly lower (p=0.04 and p=0.03, respectively) in patients having CABG performed off-pump. Starting at comparable baseline levels, the mean CEC-number was highest at 6h postoperatively with 81.9 ml(-1) (range, 44-141) for on-pump patients and 63.3 ml(-1) (range, 48-105) for off-pump patients. hEC apoptosis peaked also at T4: 16.5+/-2.8% versus 11.3+/-2.2%. In both groups, CEC numbers and proportions of endothelial apoptosis were still elevated at 48 h after surgery.

CONCLUSION

The number of circulating endothelial cells and apoptotic endothelial cell death are markers of endothelial activation and damage during CABG. This study provides evidence that CABG with the use of CPB in comparison to OPCAB surgery is associated with a significantly more pronounced endothelial response in the immediate postoperative period.

Authors+Show Affiliations

Department of Cardiothoracic and Vascular Surgery, University Hospital of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. fx.schmid@klinik.uni.regensburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

16504531

Citation

Schmid, Franz-Xaver, et al. "Endothelial Apoptosis and Circulating Endothelial Cells After Bypass Grafting With and Without Cardiopulmonary Bypass." European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery, vol. 29, no. 4, 2006, pp. 496-500.
Schmid FX, Vudattu N, Floerchinger B, et al. Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass. Eur J Cardiothorac Surg. 2006;29(4):496-500.
Schmid, F. X., Vudattu, N., Floerchinger, B., Hilker, M., Eissner, G., Hoenicka, M., Holler, E., & Birnbaum, D. E. (2006). Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass. European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery, 29(4), 496-500.
Schmid FX, et al. Endothelial Apoptosis and Circulating Endothelial Cells After Bypass Grafting With and Without Cardiopulmonary Bypass. Eur J Cardiothorac Surg. 2006;29(4):496-500. PubMed PMID: 16504531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass. AU - Schmid,Franz-Xaver, AU - Vudattu,Nalini, AU - Floerchinger,Bernhard, AU - Hilker,Michael, AU - Eissner,Günther, AU - Hoenicka,Markus, AU - Holler,Ernst, AU - Birnbaum,Dietrich E, Y1 - 2006/02/28/ PY - 2005/11/11/received PY - 2006/01/13/revised PY - 2006/01/16/accepted PY - 2006/3/1/pubmed PY - 2006/6/9/medline PY - 2006/3/1/entrez SP - 496 EP - 500 JF - European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery JO - Eur J Cardiothorac Surg VL - 29 IS - 4 N2 - OBJECTIVE: We compared profiles of the numbers of circulating endothelial cells (CEC) and the apoptosis-inducing capacity of serum samples on human endothelial cells (hEC) in on-pump and off-pump coronary artery bypass grafting (CABG) patients. METHODS: Blood samples from 30 patients undergoing CABG (randomly assigned to two groups: 15 patients off-pump and 15 on-pump (cardiopulmonary bypass, CPB)) were collected after induction of anesthesia (preoperatively), at weaning from CPB/end of bypass grafting (0 h), and 1, 6, 12, 24, and 48 h afterwards. CEC were isolated with immunomagnetic anti-CD146-coated Dynabeads, and counted in a Nageotte chamber. The apoptosis-inducing activity of serum samples on hEC was examined by a tissue culture assay system. Apoptotic and normal cells were identified using phase contrast/fluorescence microscopy after DNA dye staining. RESULTS: CEC numbers and proportions of apoptotic hEC were significantly elevated during and after surgery in both groups (p<0.01). Compared with the on-pump group, CEC and proportions of apoptotic hEC were significantly lower (p=0.04 and p=0.03, respectively) in patients having CABG performed off-pump. Starting at comparable baseline levels, the mean CEC-number was highest at 6h postoperatively with 81.9 ml(-1) (range, 44-141) for on-pump patients and 63.3 ml(-1) (range, 48-105) for off-pump patients. hEC apoptosis peaked also at T4: 16.5+/-2.8% versus 11.3+/-2.2%. In both groups, CEC numbers and proportions of endothelial apoptosis were still elevated at 48 h after surgery. CONCLUSION: The number of circulating endothelial cells and apoptotic endothelial cell death are markers of endothelial activation and damage during CABG. This study provides evidence that CABG with the use of CPB in comparison to OPCAB surgery is associated with a significantly more pronounced endothelial response in the immediate postoperative period. SN - 1010-7940 UR - https://www.unboundmedicine.com/medline/citation/16504531/Endothelial_apoptosis_and_circulating_endothelial_cells_after_bypass_grafting_with_and_without_cardiopulmonary_bypass_ L2 - https://academic.oup.com/ejcts/article-lookup/doi/10.1016/j.ejcts.2006.01.029 DB - PRIME DP - Unbound Medicine ER -