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Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated by CB1 and CB2 receptor coupled pathways.
Int Immunopharmacol. 2006 Apr; 6(4):656-65.II

Abstract

There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of Delta(9)-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent Delta(9)-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-alpha) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-alpha level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways.

Authors+Show Affiliations

TNO Pharma, Utrechtseweg 48, PZeist, The Netherlands. Verhoeckx@pharma.tno.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16504929

Citation

Verhoeckx, Kitty C M., et al. "Unheated Cannabis Sativa Extracts and Its Major Compound THC-acid Have Potential Immuno-modulating Properties Not Mediated By CB1 and CB2 Receptor Coupled Pathways." International Immunopharmacology, vol. 6, no. 4, 2006, pp. 656-65.
Verhoeckx KC, Korthout HA, van Meeteren-Kreikamp AP, et al. Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated by CB1 and CB2 receptor coupled pathways. Int Immunopharmacol. 2006;6(4):656-65.
Verhoeckx, K. C., Korthout, H. A., van Meeteren-Kreikamp, A. P., Ehlert, K. A., Wang, M., van der Greef, J., Rodenburg, R. J., & Witkamp, R. F. (2006). Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated by CB1 and CB2 receptor coupled pathways. International Immunopharmacology, 6(4), 656-65.
Verhoeckx KC, et al. Unheated Cannabis Sativa Extracts and Its Major Compound THC-acid Have Potential Immuno-modulating Properties Not Mediated By CB1 and CB2 Receptor Coupled Pathways. Int Immunopharmacol. 2006;6(4):656-65. PubMed PMID: 16504929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated by CB1 and CB2 receptor coupled pathways. AU - Verhoeckx,Kitty C M, AU - Korthout,Henrie A A J, AU - van Meeteren-Kreikamp,A P, AU - Ehlert,Karl A, AU - Wang,Mei, AU - van der Greef,Jan, AU - Rodenburg,Richard J T, AU - Witkamp,Renger F, Y1 - 2005/11/07/ PY - 2005/09/12/received PY - 2005/09/30/revised PY - 2005/10/10/accepted PY - 2006/3/1/pubmed PY - 2006/4/28/medline PY - 2006/3/1/entrez SP - 656 EP - 65 JF - International immunopharmacology JO - Int Immunopharmacol VL - 6 IS - 4 N2 - There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of Delta(9)-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent Delta(9)-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-alpha) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-alpha level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/16504929/Unheated_Cannabis_sativa_extracts_and_its_major_compound_THC_acid_have_potential_immuno_modulating_properties_not_mediated_by_CB1_and_CB2_receptor_coupled_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(05)00288-2 DB - PRIME DP - Unbound Medicine ER -