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TNF-alpha and phosphorylation of ERK in DRG and spinal cord: insights into mechanisms of sciatica.
Spine (Phila Pa 1976). 2006 Mar 01; 31(5):523-9.S

Abstract

STUDY DESIGN

Characterize extracellular signal-regulated kinase (ERK) and its phosphorylation (pERK) in neural tissues after topical application of tumor necrosis factor-alpha (TNF-alpha) to L5 nerve root.

OBJECTIVE

Identify time-course, localization, and expression of pERK.

SUMMARY OF BACKGROUND DATA

TNF-alpha has a key role in disc herniation and sciatica as an inflammatory component of the nucleus pulposus. ERK is associated with neuronal signal transduction and nociception.

METHODS

We studied tissue from naive rats, vehicle-treated rats, and rats receiving rat recombinant TNF-alpha using Western blots of total and phosphorylated ERK (pERK). We used immunohistochemistry of pERK with neuronal nuclear (NeuN) antibody to identify its cellular distribution.

RESULTS

Topical application of TNF-alpha to rat nerve root increased pERK in ipsilateral dorsal root ganglion (DRG) neurons and glia within 5 hours. pERK was not expressed in DRG during the first hour after TNF-alpha application, nor was it seen at anytime in spinal cord dorsal horn. DRG satellite cells had increased pERK 5 hours after TNF-alpha or vehicle treatment. TNF-alpha treatment increased pERK in small- and medium-sized DRG neurons and to a lesser degree in large neurons.

CONCLUSIONS

These findings suggest that ERK signaling plays a role in the activation of DRG cells following inflammatory injuries to nerve roots and further documents the importance of inflammation in the pathogenesis of painful spine disorders.

Authors+Show Affiliations

Department of Anesthesiology, University of California, School of Medicine, San Diego, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16508545

Citation

Takahashi, Naoto, et al. "TNF-alpha and Phosphorylation of ERK in DRG and Spinal Cord: Insights Into Mechanisms of Sciatica." Spine, vol. 31, no. 5, 2006, pp. 523-9.
Takahashi N, Kikuchi S, Shubayev VI, et al. TNF-alpha and phosphorylation of ERK in DRG and spinal cord: insights into mechanisms of sciatica. Spine. 2006;31(5):523-9.
Takahashi, N., Kikuchi, S., Shubayev, V. I., Campana, W. M., & Myers, R. R. (2006). TNF-alpha and phosphorylation of ERK in DRG and spinal cord: insights into mechanisms of sciatica. Spine, 31(5), 523-9.
Takahashi N, et al. TNF-alpha and Phosphorylation of ERK in DRG and Spinal Cord: Insights Into Mechanisms of Sciatica. Spine. 2006 Mar 1;31(5):523-9. PubMed PMID: 16508545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TNF-alpha and phosphorylation of ERK in DRG and spinal cord: insights into mechanisms of sciatica. AU - Takahashi,Naoto, AU - Kikuchi,Shinichi, AU - Shubayev,Veronica I, AU - Campana,W Marie, AU - Myers,Robert R, PY - 2006/3/2/pubmed PY - 2006/4/7/medline PY - 2006/3/2/entrez SP - 523 EP - 9 JF - Spine JO - Spine VL - 31 IS - 5 N2 - STUDY DESIGN: Characterize extracellular signal-regulated kinase (ERK) and its phosphorylation (pERK) in neural tissues after topical application of tumor necrosis factor-alpha (TNF-alpha) to L5 nerve root. OBJECTIVE: Identify time-course, localization, and expression of pERK. SUMMARY OF BACKGROUND DATA: TNF-alpha has a key role in disc herniation and sciatica as an inflammatory component of the nucleus pulposus. ERK is associated with neuronal signal transduction and nociception. METHODS: We studied tissue from naive rats, vehicle-treated rats, and rats receiving rat recombinant TNF-alpha using Western blots of total and phosphorylated ERK (pERK). We used immunohistochemistry of pERK with neuronal nuclear (NeuN) antibody to identify its cellular distribution. RESULTS: Topical application of TNF-alpha to rat nerve root increased pERK in ipsilateral dorsal root ganglion (DRG) neurons and glia within 5 hours. pERK was not expressed in DRG during the first hour after TNF-alpha application, nor was it seen at anytime in spinal cord dorsal horn. DRG satellite cells had increased pERK 5 hours after TNF-alpha or vehicle treatment. TNF-alpha treatment increased pERK in small- and medium-sized DRG neurons and to a lesser degree in large neurons. CONCLUSIONS: These findings suggest that ERK signaling plays a role in the activation of DRG cells following inflammatory injuries to nerve roots and further documents the importance of inflammation in the pathogenesis of painful spine disorders. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/16508545/TNF_alpha_and_phosphorylation_of_ERK_in_DRG_and_spinal_cord:_insights_into_mechanisms_of_sciatica_ L2 - http://dx.doi.org/10.1097/01.brs.0000201305.01522.17 DB - PRIME DP - Unbound Medicine ER -