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Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis.
Arthritis Rheum. 2006 Mar; 54(3):887-98.AR

Abstract

OBJECTIVE

Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA).

METHODS

CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8alpha+ DCs in the Peyer's patches of CII-fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CD11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined.

RESULTS

The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer.

CONCLUSION

Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases.

Authors+Show Affiliations

Rheumatism Research Center, The Catholic University of Korea, Seocho-Ku, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16508971

Citation

Min, So-Youn, et al. "Antigen-induced, Tolerogenic CD11c+,CD11b+ Dendritic Cells Are Abundant in Peyer's Patches During the Induction of Oral Tolerance to Type II Collagen and Suppress Experimental Collagen-induced Arthritis." Arthritis and Rheumatism, vol. 54, no. 3, 2006, pp. 887-98.
Min SY, Park KS, Cho ML, et al. Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis. Arthritis Rheum. 2006;54(3):887-98.
Min, S. Y., Park, K. S., Cho, M. L., Kang, J. W., Cho, Y. G., Hwang, S. Y., Park, M. J., Yoon, C. H., Min, J. K., Lee, S. H., Park, S. H., & Kim, H. Y. (2006). Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis. Arthritis and Rheumatism, 54(3), 887-98.
Min SY, et al. Antigen-induced, Tolerogenic CD11c+,CD11b+ Dendritic Cells Are Abundant in Peyer's Patches During the Induction of Oral Tolerance to Type II Collagen and Suppress Experimental Collagen-induced Arthritis. Arthritis Rheum. 2006;54(3):887-98. PubMed PMID: 16508971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis. AU - Min,So-Youn, AU - Park,Kyung-Su, AU - Cho,Mi-La, AU - Kang,Jung-Won, AU - Cho,Young-Gyu, AU - Hwang,Sue-Yun, AU - Park,Min-Jung, AU - Yoon,Chong-Hyeon, AU - Min,Jun-Ki, AU - Lee,Sang-Heon, AU - Park,Sung-Hwan, AU - Kim,Ho-Youn, PY - 2006/3/2/pubmed PY - 2006/4/14/medline PY - 2006/3/2/entrez SP - 887 EP - 98 JF - Arthritis and rheumatism JO - Arthritis Rheum. VL - 54 IS - 3 N2 - OBJECTIVE: Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA). METHODS: CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8alpha+ DCs in the Peyer's patches of CII-fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CD11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined. RESULTS: The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer. CONCLUSION: Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases. SN - 0004-3591 UR - https://www.unboundmedicine.com/medline/citation/16508971/Antigen_induced_tolerogenic_CD11c+CD11b+_dendritic_cells_are_abundant_in_Peyer's_patches_during_the_induction_of_oral_tolerance_to_type_II_collagen_and_suppress_experimental_collagen_induced_arthritis_ L2 - https://doi.org/10.1002/art.21647 DB - PRIME DP - Unbound Medicine ER -