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Synthesis of AgcoreAushell bimetallic nanoparticles for immunoassay based on surface-enhanced Raman spectroscopy.
J Phys Chem B. 2006 Mar 09; 110(9):4002-6.JP

Abstract

Layered core-shell bimetallic silver-gold nanoparticles were prepared by coating Au layers over Ag seeds by a seed-growth method. The composition of Ag100-xAux particles can vary from x=0 to 30. TEM and SEM images clearly show that the bimetallic nanoparticles are of core-shell structure with some pinholes on the surface. Strong surface-enhanced Raman (SER) signals of thiophenol and p-aminothiophenol have been obtained with these colloids. It was found that the SERS activity of aggregated colloids critically depends on the molar ratio of Ag to Au. With the increase of the Au molar fraction, the SERS activity enhances first and then weakens, with the maximal intensity being 10 times stronger than that of Ag colloids. The AgcoreAushell nanoparticles were then labeled with monoclonal antibodies and SERS probes and used for immunoassay analysis. In the proposed system, antibodies immobilized on a solid substrate can interact with the corresponding antigens to form a composite substrate, which can capture reporter-labeled AgcoreAushell nanoparticles modified with the same antibodies. The immunoreaction between the antibodies and antigens was demonstrated by the detection of characteristic Raman bands of the probe molecules. AgcoreAushell bimetallic nanoparticles, as a new SERS active and biocompatible substrate, will be expected to improve the detection sensitivity of immunoassay.

Authors+Show Affiliations

Cui Y
Department of Chemistry, Suzhou University, Suzhou 215006, China.
Ren B
No affiliation info available
Yao JL
No affiliation info available
Gu RA
No affiliation info available
Tian ZQ
No affiliation info available

MeSH

Antibodies, MonoclonalGoldImmunoassayMetal NanoparticlesSilverSpectrum Analysis, Raman

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16509689

Citation

Cui, Yan, et al. "Synthesis of AgcoreAushell Bimetallic Nanoparticles for Immunoassay Based On Surface-enhanced Raman Spectroscopy." The Journal of Physical Chemistry. B, vol. 110, no. 9, 2006, pp. 4002-6.
Cui Y, Ren B, Yao JL, et al. Synthesis of AgcoreAushell bimetallic nanoparticles for immunoassay based on surface-enhanced Raman spectroscopy. J Phys Chem B. 2006;110(9):4002-6.
Cui, Y., Ren, B., Yao, J. L., Gu, R. A., & Tian, Z. Q. (2006). Synthesis of AgcoreAushell bimetallic nanoparticles for immunoassay based on surface-enhanced Raman spectroscopy. The Journal of Physical Chemistry. B, 110(9), 4002-6.
Cui Y, et al. Synthesis of AgcoreAushell Bimetallic Nanoparticles for Immunoassay Based On Surface-enhanced Raman Spectroscopy. J Phys Chem B. 2006 Mar 9;110(9):4002-6. PubMed PMID: 16509689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of AgcoreAushell bimetallic nanoparticles for immunoassay based on surface-enhanced Raman spectroscopy. AU - Cui,Yan, AU - Ren,Bin, AU - Yao,Jian-Lin, AU - Gu,Ren-Ao, AU - Tian,Zhong-Qun, PY - 2006/3/3/pubmed PY - 2007/8/4/medline PY - 2006/3/3/entrez SP - 4002 EP - 6 JF - The journal of physical chemistry. B JO - J Phys Chem B VL - 110 IS - 9 N2 - Layered core-shell bimetallic silver-gold nanoparticles were prepared by coating Au layers over Ag seeds by a seed-growth method. The composition of Ag100-xAux particles can vary from x=0 to 30. TEM and SEM images clearly show that the bimetallic nanoparticles are of core-shell structure with some pinholes on the surface. Strong surface-enhanced Raman (SER) signals of thiophenol and p-aminothiophenol have been obtained with these colloids. It was found that the SERS activity of aggregated colloids critically depends on the molar ratio of Ag to Au. With the increase of the Au molar fraction, the SERS activity enhances first and then weakens, with the maximal intensity being 10 times stronger than that of Ag colloids. The AgcoreAushell nanoparticles were then labeled with monoclonal antibodies and SERS probes and used for immunoassay analysis. In the proposed system, antibodies immobilized on a solid substrate can interact with the corresponding antigens to form a composite substrate, which can capture reporter-labeled AgcoreAushell nanoparticles modified with the same antibodies. The immunoreaction between the antibodies and antigens was demonstrated by the detection of characteristic Raman bands of the probe molecules. AgcoreAushell bimetallic nanoparticles, as a new SERS active and biocompatible substrate, will be expected to improve the detection sensitivity of immunoassay. SN - 1520-6106 UR - https://www.unboundmedicine.com/medline/citation/16509689/Synthesis_of_AgcoreAushell_bimetallic_nanoparticles_for_immunoassay_based_on_surface_enhanced_Raman_spectroscopy_ DB - PRIME DP - Unbound Medicine ER -