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Comparison of two chromogenic media for the detection of Listeria monocytogenes with the plating media recommended by EN/DIN 11290-1.
Int J Food Microbiol. 2006 May 25; 109(1-2):127-31.IJ

Abstract

The efficacy of two selective chromogenic culture media, Agar Listeria Ottaviani and Agosti (ALOA) and RAPID' L. mono for the detection of Listeria monocytogenes in food, was compared with that of an official culture method according to the EN/DIN 11290-01 and -02 protocols [corresponding to the section 35 LMBG (German Food Act) method]. A total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and mixed salads) were examined. L. monocytogenes was identified in 52 investigated salmon samples. Using two chromogenic media, 50 samples were found positive for L. monocytogenes. Compared to the reference method there were no false-positive results. By the EN/DIN 11290-01 culture procedure after the selective enrichment in Fraser broth 12 out of 130 samples of sausages were positive for L. monocytogenes. These 12 samples were also positive for L. monocytogenes with the chromogenic medium RAPID' L. mono. One sample was false negative with ALOA. Three additional samples were found positive with ALOA and four with RAPID' L. mono. The standard method was inadequate to confirm these samples as positive. Listeria spp. were isolated from 7 samples of mixed salads with both methods. One, 3 and 3 samples were found to contain L. monocytogenes, L. innocua and L. seeligeri, respectively. Both chromogenic media enabled a rapid and specific detection of L. monocytogenes within 24h after enrichment. Visual detection of pathogenic L. monocytogenes and other Listeria spp. was easier on chromogenic media.

Authors+Show Affiliations

Becker B
Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Str. 9, D-76131 Karlsruhe, Germany. biserka.becker@bfel.de
Schuler S
No affiliation info available
Lohneis M
No affiliation info available
Sabrowski A
No affiliation info available
Curtis GD
No affiliation info available
Holzapfel WH
No affiliation info available

MeSH

AgarAnimalsChromogenic CompoundsColony Count, MicrobialConsumer Product SafetyCulture MediaFalse Negative ReactionsFalse Positive ReactionsFish ProductsFood ContaminationFood MicrobiologyHumansLettuceListeria monocytogenesMeat Products

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16515816

Citation

Becker, Biserka, et al. "Comparison of Two Chromogenic Media for the Detection of Listeria Monocytogenes With the Plating Media Recommended By EN/DIN 11290-1." International Journal of Food Microbiology, vol. 109, no. 1-2, 2006, pp. 127-31.
Becker B, Schuler S, Lohneis M, et al. Comparison of two chromogenic media for the detection of Listeria monocytogenes with the plating media recommended by EN/DIN 11290-1. Int J Food Microbiol. 2006;109(1-2):127-31.
Becker, B., Schuler, S., Lohneis, M., Sabrowski, A., Curtis, G. D., & Holzapfel, W. H. (2006). Comparison of two chromogenic media for the detection of Listeria monocytogenes with the plating media recommended by EN/DIN 11290-1. International Journal of Food Microbiology, 109(1-2), 127-31.
Becker B, et al. Comparison of Two Chromogenic Media for the Detection of Listeria Monocytogenes With the Plating Media Recommended By EN/DIN 11290-1. Int J Food Microbiol. 2006 May 25;109(1-2):127-31. PubMed PMID: 16515816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of two chromogenic media for the detection of Listeria monocytogenes with the plating media recommended by EN/DIN 11290-1. AU - Becker,Biserka, AU - Schuler,Stefanie, AU - Lohneis,Martin, AU - Sabrowski,Annemarie, AU - Curtis,Gordon D W, AU - Holzapfel,Wilhelm H, Y1 - 2006/03/03/ PY - 2005/09/28/received PY - 2005/12/13/revised PY - 2006/01/03/accepted PY - 2006/3/7/pubmed PY - 2006/8/8/medline PY - 2006/3/7/entrez SP - 127 EP - 31 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 109 IS - 1-2 N2 - The efficacy of two selective chromogenic culture media, Agar Listeria Ottaviani and Agosti (ALOA) and RAPID' L. mono for the detection of Listeria monocytogenes in food, was compared with that of an official culture method according to the EN/DIN 11290-01 and -02 protocols [corresponding to the section 35 LMBG (German Food Act) method]. A total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and mixed salads) were examined. L. monocytogenes was identified in 52 investigated salmon samples. Using two chromogenic media, 50 samples were found positive for L. monocytogenes. Compared to the reference method there were no false-positive results. By the EN/DIN 11290-01 culture procedure after the selective enrichment in Fraser broth 12 out of 130 samples of sausages were positive for L. monocytogenes. These 12 samples were also positive for L. monocytogenes with the chromogenic medium RAPID' L. mono. One sample was false negative with ALOA. Three additional samples were found positive with ALOA and four with RAPID' L. mono. The standard method was inadequate to confirm these samples as positive. Listeria spp. were isolated from 7 samples of mixed salads with both methods. One, 3 and 3 samples were found to contain L. monocytogenes, L. innocua and L. seeligeri, respectively. Both chromogenic media enabled a rapid and specific detection of L. monocytogenes within 24h after enrichment. Visual detection of pathogenic L. monocytogenes and other Listeria spp. was easier on chromogenic media. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/16515816/Comparison_of_two_chromogenic_media_for_the_detection_of_Listeria_monocytogenes_with_the_plating_media_recommended_by_EN/DIN_11290_1_ DB - PRIME DP - Unbound Medicine ER -