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Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells.
J Nutr Biochem. 2006 Oct; 17(10):689-96.JN

Abstract

Isoliquiritigenin (ISL), a simple chalcone derivative, 4,2',4'-trihydroxychalcone, found in licorice, shallot and bean sprouts, has been reported to have chemoprotective effects. To examine the effects of ISL on the growth of prostate cancer cells, we cultured MAT-LyLu (MLL) rat and DU145 human prostate cancer cells with various concentrations (0-20 micromol/L) of ISL. Treatment of the cells with increasing concentrations of ISL led to dose-dependent decreases in the viable cell numbers in both DU145 and MLL cells (P<.05). Hoechst 33258 dye staining of condensed nuclei and annexin V binding to surface phosphatidylserine revealed increased numbers of apoptotic cells after ISL treatment. Western blot analysis revealed that ISL increased the levels of membrane-bound Fas ligand (FasL), Fas, cleaved casapse-8, truncated Bid (tBid), Bax and Bad in DU145 cells (P<.05). Isoliquiritigenin increased the percentage of cells with depolarized mitochondrial membranes, in a concentration-dependent manner (P<.05). Isoliquiritigenin induced the release of cytochrome c and Smac/Diablo from the mitochondria into the cytoplasm (P<.05). Isoliquiritigenin dose-dependently increased the levels of cleaved caspase-9, caspase-7, caspase-3 and poly(ADP-ribose) polymerase (P<.05). The present results indicate that ISL inhibits prostate cancer cell growth by the induction of apoptosis, which is mediated through mitochondrial events, which are associated with an evident disruption of the mitochondrial membrane potential, and the release of cytochrome c and Smac/Diablo, and the activation of caspase-9.

Authors+Show Affiliations

Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16517140

Citation

Jung, Jae In, et al. "Isoliquiritigenin Induces Apoptosis By Depolarizing Mitochondrial Membranes in Prostate Cancer Cells." The Journal of Nutritional Biochemistry, vol. 17, no. 10, 2006, pp. 689-96.
Jung JI, Lim SS, Choi HJ, et al. Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells. J Nutr Biochem. 2006;17(10):689-96.
Jung, J. I., Lim, S. S., Choi, H. J., Cho, H. J., Shin, H. K., Kim, E. J., Chung, W. Y., Park, K. K., & Park, J. H. (2006). Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells. The Journal of Nutritional Biochemistry, 17(10), 689-96.
Jung JI, et al. Isoliquiritigenin Induces Apoptosis By Depolarizing Mitochondrial Membranes in Prostate Cancer Cells. J Nutr Biochem. 2006;17(10):689-96. PubMed PMID: 16517140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoliquiritigenin induces apoptosis by depolarizing mitochondrial membranes in prostate cancer cells. AU - Jung,Jae In, AU - Lim,Soon Sung, AU - Choi,Hyun Ju, AU - Cho,Han Jin, AU - Shin,Hyun-Kyung, AU - Kim,Eun Ji, AU - Chung,Won-Yoon, AU - Park,Kwang-Kyun, AU - Park,Jung Han Yoon, Y1 - 2005/12/09/ PY - 2005/10/13/received PY - 2005/11/08/revised PY - 2005/11/09/accepted PY - 2006/3/7/pubmed PY - 2006/11/4/medline PY - 2006/3/7/entrez SP - 689 EP - 96 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 17 IS - 10 N2 - Isoliquiritigenin (ISL), a simple chalcone derivative, 4,2',4'-trihydroxychalcone, found in licorice, shallot and bean sprouts, has been reported to have chemoprotective effects. To examine the effects of ISL on the growth of prostate cancer cells, we cultured MAT-LyLu (MLL) rat and DU145 human prostate cancer cells with various concentrations (0-20 micromol/L) of ISL. Treatment of the cells with increasing concentrations of ISL led to dose-dependent decreases in the viable cell numbers in both DU145 and MLL cells (P<.05). Hoechst 33258 dye staining of condensed nuclei and annexin V binding to surface phosphatidylserine revealed increased numbers of apoptotic cells after ISL treatment. Western blot analysis revealed that ISL increased the levels of membrane-bound Fas ligand (FasL), Fas, cleaved casapse-8, truncated Bid (tBid), Bax and Bad in DU145 cells (P<.05). Isoliquiritigenin increased the percentage of cells with depolarized mitochondrial membranes, in a concentration-dependent manner (P<.05). Isoliquiritigenin induced the release of cytochrome c and Smac/Diablo from the mitochondria into the cytoplasm (P<.05). Isoliquiritigenin dose-dependently increased the levels of cleaved caspase-9, caspase-7, caspase-3 and poly(ADP-ribose) polymerase (P<.05). The present results indicate that ISL inhibits prostate cancer cell growth by the induction of apoptosis, which is mediated through mitochondrial events, which are associated with an evident disruption of the mitochondrial membrane potential, and the release of cytochrome c and Smac/Diablo, and the activation of caspase-9. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/16517140/Isoliquiritigenin_induces_apoptosis_by_depolarizing_mitochondrial_membranes_in_prostate_cancer_cells_ DB - PRIME DP - Unbound Medicine ER -