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Natural autoantibodies against glomerular basement membrane exist in normal human sera.
Kidney Int. 2006 Mar; 69(5):894-9.KI

Abstract

Autoimmunity to glomerular basement membrane (GBM) could induce Goodpasture disease, and natural autoantibodies against GBM in the sera of normal individuals were not reported. The aim of the study was to identify and characterize natural autoantibodies against GBM in normal human sera. Natural anti-GBM autoantibodies were purified from the sera of five healthy persons by affinity chromatography, using purified bovine alpha(IV)non-collagenous (NC1) as solid-phase ligands. Antigen specificity, immunoglobulin G (IgG) subclasses, and antibody avidity of the natural autoantibodies were investigated by enzyme-linked immunosorbant assay (ELISA), Western-blot analysis, indirect immunofluorescence, and antigen-inhibition ELISA, and compared with those of 32 patients with anti-GBM disease. Natural anti-GBM autoantibodies could be purified from IgG fractions of all the five persons, with an average amount of 0.5% of total IgG fractions. Antigen specificity of the natural autoantibodies was identified by blotting to human alpha(IV)NC1, reactivity to recombinant alpha3(IV)NC1, and linear staining along the GBM of normal kidney sections. Titers of the natural autoantibodies were much lower than those of patients (1:60.6 vs 1:993.6, P<0.001). IgG subclasses distribution of the natural autoantibodies was restricted to IgG2 (100%) and IgG4 (100%), while for patients it was mainly IgG1 (93.8%) and IgG4 (90.6%). Avidity of the natural autoantibodies was lower than that of patients, the amount of alpha(IV)NC1 used for 50% inhibition was 1.65 and 0.46 microg, respectively (P<0.05). In conclusion, natural anti-GBM autoantibodies exist in normal human sera. Antibody levels, IgG subclasses, and avidity of the natural autoantibodies were different from those of patients. Fine specificity of the natural autoantibodies needed to be elucidated.

Authors+Show Affiliations

Renal Division & Institute of Nephrology, Peking University First Hospital, Beijing 100-034, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16518348

Citation

Cui, Z, et al. "Natural Autoantibodies Against Glomerular Basement Membrane Exist in Normal Human Sera." Kidney International, vol. 69, no. 5, 2006, pp. 894-9.
Cui Z, Wang HY, Zhao MH. Natural autoantibodies against glomerular basement membrane exist in normal human sera. Kidney Int. 2006;69(5):894-9.
Cui, Z., Wang, H. Y., & Zhao, M. H. (2006). Natural autoantibodies against glomerular basement membrane exist in normal human sera. Kidney International, 69(5), 894-9.
Cui Z, Wang HY, Zhao MH. Natural Autoantibodies Against Glomerular Basement Membrane Exist in Normal Human Sera. Kidney Int. 2006;69(5):894-9. PubMed PMID: 16518348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural autoantibodies against glomerular basement membrane exist in normal human sera. AU - Cui,Z, AU - Wang,H-Y, AU - Zhao,M-H, PY - 2006/3/7/pubmed PY - 2006/5/25/medline PY - 2006/3/7/entrez SP - 894 EP - 9 JF - Kidney international JO - Kidney Int VL - 69 IS - 5 N2 - Autoimmunity to glomerular basement membrane (GBM) could induce Goodpasture disease, and natural autoantibodies against GBM in the sera of normal individuals were not reported. The aim of the study was to identify and characterize natural autoantibodies against GBM in normal human sera. Natural anti-GBM autoantibodies were purified from the sera of five healthy persons by affinity chromatography, using purified bovine alpha(IV)non-collagenous (NC1) as solid-phase ligands. Antigen specificity, immunoglobulin G (IgG) subclasses, and antibody avidity of the natural autoantibodies were investigated by enzyme-linked immunosorbant assay (ELISA), Western-blot analysis, indirect immunofluorescence, and antigen-inhibition ELISA, and compared with those of 32 patients with anti-GBM disease. Natural anti-GBM autoantibodies could be purified from IgG fractions of all the five persons, with an average amount of 0.5% of total IgG fractions. Antigen specificity of the natural autoantibodies was identified by blotting to human alpha(IV)NC1, reactivity to recombinant alpha3(IV)NC1, and linear staining along the GBM of normal kidney sections. Titers of the natural autoantibodies were much lower than those of patients (1:60.6 vs 1:993.6, P<0.001). IgG subclasses distribution of the natural autoantibodies was restricted to IgG2 (100%) and IgG4 (100%), while for patients it was mainly IgG1 (93.8%) and IgG4 (90.6%). Avidity of the natural autoantibodies was lower than that of patients, the amount of alpha(IV)NC1 used for 50% inhibition was 1.65 and 0.46 microg, respectively (P<0.05). In conclusion, natural anti-GBM autoantibodies exist in normal human sera. Antibody levels, IgG subclasses, and avidity of the natural autoantibodies were different from those of patients. Fine specificity of the natural autoantibodies needed to be elucidated. SN - 0085-2538 UR - https://www.unboundmedicine.com/medline/citation/16518348/Natural_autoantibodies_against_glomerular_basement_membrane_exist_in_normal_human_sera_ DB - PRIME DP - Unbound Medicine ER -