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Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers.
Theor Appl Genet. 2006 May; 112(7):1374-81.TA

Abstract

Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, molecular markers linked to these genes have not been identified and linkage relationships of the two genes with other important genes in wheat have not been established. We observed that the F1 hybrids from the crosses between the bread wheat variety 'Alsen' and four synthetic hexaploid wheat (SHW) lines (TA4152-19, TA4152-37, TA4152-44, and TA4152-60) developed at the International Maize and Wheat Improvement Center (CIMMYT) exhibited hybrid necrosis. This study was conducted to determine the genotypes of TA4152-60 and Alsen at the Ne1 and Ne2 loci, and to map the genes using microsatellite markers in backcross populations. Genetic analysis indicated that Alsen has the genotype ne1ne1Ne2Ne2 whereas the SHW lines have Ne1Ne1ne2ne2. The microsatellite marker Xbarc74 was linked to Ne1 at a genetic distance of 2.0 cM on chromosome arm 5BL, and Xbarc55 was 3.2 cM from Ne2 on 2BS. Comparison of the genetic maps with the chromosome deletion-based physical maps indicated that Ne1 lies in the proximal half of 5BL, whereas Ne2 is in the distal half of 2BS. Genetic linkage analysis showed that Ne1 was about 35 cM proximal to Tsn1, a locus conferring sensitivity to the host selective toxin Ptr ToxA produced by the tan spot fungus. The closely linked microsatellite markers identified in this study can be used to genotype parental lines for Ne1 and Ne2 or to eliminate the two hybrid necrosis genes using marker-assisted selection.

Authors+Show Affiliations

Department of Plant Sciences, North Dakota State University, Fargo, ND 58105, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16518615

Citation

Chu, C-G, et al. "Molecular Mapping of Hybrid Necrosis Genes Ne1 and Ne2 in Hexaploid Wheat Using Microsatellite Markers." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 112, no. 7, 2006, pp. 1374-81.
Chu CG, Faris JD, Friesen TL, et al. Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers. Theor Appl Genet. 2006;112(7):1374-81.
Chu, C. G., Faris, J. D., Friesen, T. L., & Xu, S. S. (2006). Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 112(7), 1374-81.
Chu CG, et al. Molecular Mapping of Hybrid Necrosis Genes Ne1 and Ne2 in Hexaploid Wheat Using Microsatellite Markers. Theor Appl Genet. 2006;112(7):1374-81. PubMed PMID: 16518615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mapping of hybrid necrosis genes Ne1 and Ne2 in hexaploid wheat using microsatellite markers. AU - Chu,C-G, AU - Faris,J D, AU - Friesen,T L, AU - Xu,S S, Y1 - 2006/03/04/ PY - 2005/11/03/received PY - 2006/02/05/accepted PY - 2006/3/7/pubmed PY - 2006/6/30/medline PY - 2006/3/7/entrez SP - 1374 EP - 81 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 112 IS - 7 N2 - Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, molecular markers linked to these genes have not been identified and linkage relationships of the two genes with other important genes in wheat have not been established. We observed that the F1 hybrids from the crosses between the bread wheat variety 'Alsen' and four synthetic hexaploid wheat (SHW) lines (TA4152-19, TA4152-37, TA4152-44, and TA4152-60) developed at the International Maize and Wheat Improvement Center (CIMMYT) exhibited hybrid necrosis. This study was conducted to determine the genotypes of TA4152-60 and Alsen at the Ne1 and Ne2 loci, and to map the genes using microsatellite markers in backcross populations. Genetic analysis indicated that Alsen has the genotype ne1ne1Ne2Ne2 whereas the SHW lines have Ne1Ne1ne2ne2. The microsatellite marker Xbarc74 was linked to Ne1 at a genetic distance of 2.0 cM on chromosome arm 5BL, and Xbarc55 was 3.2 cM from Ne2 on 2BS. Comparison of the genetic maps with the chromosome deletion-based physical maps indicated that Ne1 lies in the proximal half of 5BL, whereas Ne2 is in the distal half of 2BS. Genetic linkage analysis showed that Ne1 was about 35 cM proximal to Tsn1, a locus conferring sensitivity to the host selective toxin Ptr ToxA produced by the tan spot fungus. The closely linked microsatellite markers identified in this study can be used to genotype parental lines for Ne1 and Ne2 or to eliminate the two hybrid necrosis genes using marker-assisted selection. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16518615/Molecular_mapping_of_hybrid_necrosis_genes_Ne1_and_Ne2_in_hexaploid_wheat_using_microsatellite_markers_ L2 - https://dx.doi.org/10.1007/s00122-006-0239-9 DB - PRIME DP - Unbound Medicine ER -