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[Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia].
Zhonghua Nan Ke Xue. 2006 Feb; 12(2):108-11.ZN

Abstract

OBJECTIVE

To observe the relationship between microdeletions of AZF(azoospermia factor) on Y chromosome in male with idiopathic azoospermia and severe oligozoospermia.

METHODS

Only patients with an apparently normal 46,XY karyotype and normal FSH, LH and T were included in this study. Multiplex PCR was used to detect the sequence-tagged sites(STS) as follows :sY84, sY86, sY127, sY134, sY152, sY153, sY254, sY255, and ZFX/Y was used as internal control gene.

RESULTS

No microdeletion was detected in the control whereas 8 microdeletion cases existed in 67 idiopathic azoospermia and severe oligozoospermia, including 4 in AZFc, 2 in AZFa + AZFc, 1 in AZFc + AZFb, and 1 in AZFb. The prevalence rate of microdeletion was 11.94%, which was statistically different from the control.

CONCLUSION

Microdeletions in the AZF regions on the long arm of the Y-chromosome are associated with idiopathic azoospermic and severely oligozoospermic men. Multiplex PCR was a rapid and reliable method for screening microdeletions of AZF.

Authors+Show Affiliations

Key Laboratory for Improving Birth Outcome Technique of Shandong Provincial Institute of Family Planning Resaerch, Jinan, Shandong 250002, China. jyf690629@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
English Abstract
Journal Article

Language

chi

PubMed ID

16519142

Citation

Jia, Yi-Fang, et al. "[Azoospermia Factor Microdeletions in Idiopathic Azoospermia and Severe Oligozoospermia]." Zhonghua Nan Ke Xue = National Journal of Andrology, vol. 12, no. 2, 2006, pp. 108-11.
Jia YF, Wu AH, Qiu Y, et al. [Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia]. Zhonghua Nan Ke Xue. 2006;12(2):108-11.
Jia, Y. F., Wu, A. H., Qiu, Y., Qu, J. Y., & Song, C. Z. (2006). [Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia]. Zhonghua Nan Ke Xue = National Journal of Andrology, 12(2), 108-11.
Jia YF, et al. [Azoospermia Factor Microdeletions in Idiopathic Azoospermia and Severe Oligozoospermia]. Zhonghua Nan Ke Xue. 2006;12(2):108-11. PubMed PMID: 16519142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia]. AU - Jia,Yi-Fang, AU - Wu,Ai-Hua, AU - Qiu,Yi, AU - Qu,Ji-Ying, AU - Song,Chang-Zheng, PY - 2006/3/8/pubmed PY - 2007/10/12/medline PY - 2006/3/8/entrez SP - 108 EP - 11 JF - Zhonghua nan ke xue = National journal of andrology JO - Zhonghua Nan Ke Xue VL - 12 IS - 2 N2 - OBJECTIVE: To observe the relationship between microdeletions of AZF(azoospermia factor) on Y chromosome in male with idiopathic azoospermia and severe oligozoospermia. METHODS: Only patients with an apparently normal 46,XY karyotype and normal FSH, LH and T were included in this study. Multiplex PCR was used to detect the sequence-tagged sites(STS) as follows :sY84, sY86, sY127, sY134, sY152, sY153, sY254, sY255, and ZFX/Y was used as internal control gene. RESULTS: No microdeletion was detected in the control whereas 8 microdeletion cases existed in 67 idiopathic azoospermia and severe oligozoospermia, including 4 in AZFc, 2 in AZFa + AZFc, 1 in AZFc + AZFb, and 1 in AZFb. The prevalence rate of microdeletion was 11.94%, which was statistically different from the control. CONCLUSION: Microdeletions in the AZF regions on the long arm of the Y-chromosome are associated with idiopathic azoospermic and severely oligozoospermic men. Multiplex PCR was a rapid and reliable method for screening microdeletions of AZF. SN - 1009-3591 UR - https://www.unboundmedicine.com/medline/citation/16519142/[Azoospermia_factor_microdeletions_in_idiopathic_azoospermia_and_severe_oligozoospermia]_ DB - PRIME DP - Unbound Medicine ER -