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Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi.
Biochemistry 2006; 45(10):3348-56B

Abstract

Araneoid spiders use specialized abdominal glands to produce up to seven different protein-based silks/glues that have various mechanical properties. To date, the fibroin sequences encoding egg case fibers have not been fully determined. To gain further understanding of a recently reported spider silk protein gene family, several novel strategies were utilized in this study to isolate two full-length cDNAs of egg case silk proteins, cylindrical silk protein 1 (CySp1, 9.1 kb) and cylindrical silk protein 2 (CySp2, 9.8 kb), from the wasp spider, Argiope bruennichi. Northern blotting analysis demonstrated that CySp1 and CySp2 are selectively expressed in the cylindrical glands. The amino acid composition of raw egg case silk was closely consistent with the deduced amino acid composition based on the sequences of CySp1 and CySp2, which supports the assertion that CySp1 and CySp2 represent two major components of egg case silk. CySp1 and CySp2 are primarily composed of remarkable homogeneous assemble repeats that are 180 residues in length and consist of several complex subrepeats, and they contain highly homologous C-termini and markedly different N-termini. Our results suggest a possible link between CySp1 and CySp2. In addition, comparisons of stress/strain curves for dragline and egg case silk from Argiope bruennichi showed obvious differences in ultimate strength and extensibility, and similarities in toughness.

Authors+Show Affiliations

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda 386-8567, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16519529

Citation

Zhao, Ai-Chun, et al. "Novel Molecular and Mechanical Properties of Egg Case Silk From Wasp Spider, Argiope Bruennichi." Biochemistry, vol. 45, no. 10, 2006, pp. 3348-56.
Zhao AC, Zhao TF, Nakagaki K, et al. Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi. Biochemistry. 2006;45(10):3348-56.
Zhao, A. C., Zhao, T. F., Nakagaki, K., Zhang, Y. S., Sima, Y. H., Miao, Y. G., ... Nakagaki, M. (2006). Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi. Biochemistry, 45(10), pp. 3348-56.
Zhao AC, et al. Novel Molecular and Mechanical Properties of Egg Case Silk From Wasp Spider, Argiope Bruennichi. Biochemistry. 2006 Mar 14;45(10):3348-56. PubMed PMID: 16519529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi. AU - Zhao,Ai-Chun, AU - Zhao,Tian-Fu, AU - Nakagaki,Koichi, AU - Zhang,Yuan-Song, AU - Sima,Yang-Hu, AU - Miao,Yun-Gen, AU - Shiomi,Kunihiro, AU - Kajiura,Zenta, AU - Nagata,Yoko, AU - Takadera,Masayuki, AU - Nakagaki,Masao, PY - 2006/3/8/pubmed PY - 2006/5/4/medline PY - 2006/3/8/entrez SP - 3348 EP - 56 JF - Biochemistry JO - Biochemistry VL - 45 IS - 10 N2 - Araneoid spiders use specialized abdominal glands to produce up to seven different protein-based silks/glues that have various mechanical properties. To date, the fibroin sequences encoding egg case fibers have not been fully determined. To gain further understanding of a recently reported spider silk protein gene family, several novel strategies were utilized in this study to isolate two full-length cDNAs of egg case silk proteins, cylindrical silk protein 1 (CySp1, 9.1 kb) and cylindrical silk protein 2 (CySp2, 9.8 kb), from the wasp spider, Argiope bruennichi. Northern blotting analysis demonstrated that CySp1 and CySp2 are selectively expressed in the cylindrical glands. The amino acid composition of raw egg case silk was closely consistent with the deduced amino acid composition based on the sequences of CySp1 and CySp2, which supports the assertion that CySp1 and CySp2 represent two major components of egg case silk. CySp1 and CySp2 are primarily composed of remarkable homogeneous assemble repeats that are 180 residues in length and consist of several complex subrepeats, and they contain highly homologous C-termini and markedly different N-termini. Our results suggest a possible link between CySp1 and CySp2. In addition, comparisons of stress/strain curves for dragline and egg case silk from Argiope bruennichi showed obvious differences in ultimate strength and extensibility, and similarities in toughness. SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/16519529/Novel_molecular_and_mechanical_properties_of_egg_case_silk_from_wasp_spider_Argiope_bruennichi_ L2 - https://dx.doi.org/10.1021/bi052414g DB - PRIME DP - Unbound Medicine ER -