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Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice.
Free Radic Biol Med. 2006 Mar 01; 40(5):740-53.FR

Abstract

Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds, namely flavonoids and phenolic acids. Since polyphenolic compounds have a high antioxidant potential, in this study we evaluated the effect of H. perforatum in an animal model of acute inflammation, carrageenan-induced pleurisy. We report here that H. perforatum extract (given at 30 mg/kg orally, bolus prior to carrageenan) exerts potent anti-inflammatory effects in an animal model of acute inflammation. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of neutrophils (PMNs) as well as an infiltration of PMNs in lung tissues and subsequent lipid peroxidation (as determined by thiobarbituric acid-reactant substance measurement) and increased production of tumor necrosis factor-alpha, (TNF-alpha) and interleukin-1beta (IL-1 beta). All parameters of inflammation were attenuated by H. perforatum extract. Furthermore, carrageenan induced an upregulation of the expression of adhesion molecules ICAM-1, as well as an increase in the amounts of nitrotyrosine and poly(ADP-ribose) (PAR), as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, nitrotyrosine, and PAR was significantly reduced by H. perforatum extract. Additionally, we demonstrate that these inflammatory events were associated with the activation of nuclear factor-kappaB (NF-kappaB) and signal transducer and activator transcription-3 (STAT-31) activation in the lung. NF-kappaB and STAT-3 activation were significantly inhibited by H. perforatum extract treatment. Taken together, our results indicate that prevention of the activation of NF-kappaB and STAT-3 by H. perforatum extract reduces the development of acute inflammation.

Authors+Show Affiliations

Biochemistry Division, Department of Neuroscience and Vision, University of Verona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16520227

Citation

Menegazzi, Marta, et al. "Hypericum Perforatum Attenuates the Development of Carrageenan-induced Lung Injury in Mice." Free Radical Biology & Medicine, vol. 40, no. 5, 2006, pp. 740-53.
Menegazzi M, Di Paola R, Mazzon E, et al. Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. Free Radic Biol Med. 2006;40(5):740-53.
Menegazzi, M., Di Paola, R., Mazzon, E., Muià, C., Genovese, T., Crisafulli, C., Suzuki, H., & Cuzzocrea, S. (2006). Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. Free Radical Biology & Medicine, 40(5), 740-53.
Menegazzi M, et al. Hypericum Perforatum Attenuates the Development of Carrageenan-induced Lung Injury in Mice. Free Radic Biol Med. 2006 Mar 1;40(5):740-53. PubMed PMID: 16520227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. AU - Menegazzi,Marta, AU - Di Paola,Rosanna, AU - Mazzon,Emanuela, AU - Muià,Carmelo, AU - Genovese,Tiziana, AU - Crisafulli,Concetta, AU - Suzuki,Hisanori, AU - Cuzzocrea,Salvatore, Y1 - 2005/11/10/ PY - 2005/05/26/received PY - 2005/08/19/revised PY - 2005/08/19/accepted PY - 2006/3/8/pubmed PY - 2006/7/4/medline PY - 2006/3/8/entrez SP - 740 EP - 53 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 40 IS - 5 N2 - Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds, namely flavonoids and phenolic acids. Since polyphenolic compounds have a high antioxidant potential, in this study we evaluated the effect of H. perforatum in an animal model of acute inflammation, carrageenan-induced pleurisy. We report here that H. perforatum extract (given at 30 mg/kg orally, bolus prior to carrageenan) exerts potent anti-inflammatory effects in an animal model of acute inflammation. Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity which contained a large number of neutrophils (PMNs) as well as an infiltration of PMNs in lung tissues and subsequent lipid peroxidation (as determined by thiobarbituric acid-reactant substance measurement) and increased production of tumor necrosis factor-alpha, (TNF-alpha) and interleukin-1beta (IL-1 beta). All parameters of inflammation were attenuated by H. perforatum extract. Furthermore, carrageenan induced an upregulation of the expression of adhesion molecules ICAM-1, as well as an increase in the amounts of nitrotyrosine and poly(ADP-ribose) (PAR), as determined by immunohistochemical analysis of lung tissues. The degree of staining for the ICAM-1, nitrotyrosine, and PAR was significantly reduced by H. perforatum extract. Additionally, we demonstrate that these inflammatory events were associated with the activation of nuclear factor-kappaB (NF-kappaB) and signal transducer and activator transcription-3 (STAT-31) activation in the lung. NF-kappaB and STAT-3 activation were significantly inhibited by H. perforatum extract treatment. Taken together, our results indicate that prevention of the activation of NF-kappaB and STAT-3 by H. perforatum extract reduces the development of acute inflammation. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/16520227/Hypericum_perforatum_attenuates_the_development_of_carrageenan_induced_lung_injury_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(05)00493-4 DB - PRIME DP - Unbound Medicine ER -