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Suppression of pancreatitis-related allodynia/hyperalgesia by proteinase-activated receptor-2 in mice.
Br J Pharmacol. 2006 May; 148(1):54-60.BJ

Abstract

1 Proteinase-activated receptor-2 (PAR2), a receptor activated by trypsin and tryptase, is abundantly expressed in the gastrointestinal tract including the C-fiber terminal, and might play a role in processing of visceral pain. In the present study, we examined and characterized the roles of PAR2 in pancreatitis-related abdominal hyperalgesia/allodynia in mice. 2 Caerulein, administered i.p. once, caused a small increase in abdominal sensitivity to stimulation with von Frey hairs, without causing pancreatitis, in PAR2-knockout (KO) mice, but not wild-type (WT) mice. 3 Caerulein, given hourly six times in total, caused more profound abdominal hyperalgesia/allodynia in PAR2-KO mice, as compared with WT mice, although no significant differences were detected in the severity of pancreatitis between the KO and WT animals. 4 The PAR2-activating peptide, 2-furoyl-LIGRL-NH(2), coadministered repeatedly with caerulein six times in total, abolished the caerulein-evoked abdominal hyperalgesia/allodynia in WT, but not PAR2-KO, mice. Repeated doses of 2-furoyl-LIGRL-NH(2) moderately attenuated the severity of caerulein-induced pancreatitis in WT animals. 5 Our data from experiments using PAR2-KO mice provide evidence that PAR2 functions to attenuate pancreatitis-related abdominal hyperalgesia/allodynia without affecting pancreatitis itself, although the PAR2AP applied exogenously is not only antinociceptive but also anti-inflammatory.

Authors+Show Affiliations

Division of Physiology and Pathophysiology, School of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan. kawabata@phar.kindai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16520745

Citation

Kawabata, Atsufumi, et al. "Suppression of Pancreatitis-related Allodynia/hyperalgesia By Proteinase-activated Receptor-2 in Mice." British Journal of Pharmacology, vol. 148, no. 1, 2006, pp. 54-60.
Kawabata A, Matsunami M, Tsutsumi M, et al. Suppression of pancreatitis-related allodynia/hyperalgesia by proteinase-activated receptor-2 in mice. Br J Pharmacol. 2006;148(1):54-60.
Kawabata, A., Matsunami, M., Tsutsumi, M., Ishiki, T., Fukushima, O., Sekiguchi, F., Kawao, N., Minami, T., Kanke, T., & Saito, N. (2006). Suppression of pancreatitis-related allodynia/hyperalgesia by proteinase-activated receptor-2 in mice. British Journal of Pharmacology, 148(1), 54-60.
Kawabata A, et al. Suppression of Pancreatitis-related Allodynia/hyperalgesia By Proteinase-activated Receptor-2 in Mice. Br J Pharmacol. 2006;148(1):54-60. PubMed PMID: 16520745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of pancreatitis-related allodynia/hyperalgesia by proteinase-activated receptor-2 in mice. AU - Kawabata,Atsufumi, AU - Matsunami,Maho, AU - Tsutsumi,Masahiro, AU - Ishiki,Tsuyoshi, AU - Fukushima,Osamu, AU - Sekiguchi,Fumiko, AU - Kawao,Naoyuki, AU - Minami,Takeshi, AU - Kanke,Toru, AU - Saito,Naohiro, PY - 2006/3/8/pubmed PY - 2007/8/19/medline PY - 2006/3/8/entrez SP - 54 EP - 60 JF - British journal of pharmacology JO - Br J Pharmacol VL - 148 IS - 1 N2 - 1 Proteinase-activated receptor-2 (PAR2), a receptor activated by trypsin and tryptase, is abundantly expressed in the gastrointestinal tract including the C-fiber terminal, and might play a role in processing of visceral pain. In the present study, we examined and characterized the roles of PAR2 in pancreatitis-related abdominal hyperalgesia/allodynia in mice. 2 Caerulein, administered i.p. once, caused a small increase in abdominal sensitivity to stimulation with von Frey hairs, without causing pancreatitis, in PAR2-knockout (KO) mice, but not wild-type (WT) mice. 3 Caerulein, given hourly six times in total, caused more profound abdominal hyperalgesia/allodynia in PAR2-KO mice, as compared with WT mice, although no significant differences were detected in the severity of pancreatitis between the KO and WT animals. 4 The PAR2-activating peptide, 2-furoyl-LIGRL-NH(2), coadministered repeatedly with caerulein six times in total, abolished the caerulein-evoked abdominal hyperalgesia/allodynia in WT, but not PAR2-KO, mice. Repeated doses of 2-furoyl-LIGRL-NH(2) moderately attenuated the severity of caerulein-induced pancreatitis in WT animals. 5 Our data from experiments using PAR2-KO mice provide evidence that PAR2 functions to attenuate pancreatitis-related abdominal hyperalgesia/allodynia without affecting pancreatitis itself, although the PAR2AP applied exogenously is not only antinociceptive but also anti-inflammatory. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/16520745/Suppression_of_pancreatitis_related_allodynia/hyperalgesia_by_proteinase_activated_receptor_2_in_mice_ L2 - https://doi.org/10.1038/sj.bjp.0706708 DB - PRIME DP - Unbound Medicine ER -