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Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits.
Mol Nutr Food Res 2006; 50(3):282-90MN

Abstract

Oranges are clinically relevant allergenic foods. To date, orange allergens have not been characterized in detail. The study is aimed at analyzing the sensitization profile in orange-sensitized subjects with and without clinical allergy, and to identify orange allergens. Fifty-six sensitized subjects with self-reported reactions to orange were grouped into reactors (anaphylaxis or multiple episodes of immediate reactions and/or positive challenge tests) and non-reactors (negative open food challenge tests). Allergens were characterized by IgE immunoblotting, N-terminal sequencing, IgE-inhibition assays, and mediator release assays were performed to determine the allergenic potency of orange profilin. Of 56 subjects, 23 were classified as orange allergic showing mainly an oral allergy syndrome. Of 23 subjects classified as orange allergic, 22 were sensitized to profilin, Cit s 2. In patients with mono-sensitization to profilin in vitro histamine releases up to 75% from basophils were induced using orange extract and purified plant profilins. Of the allergic patients 78% were sensitized to germin-like protein, Cit s 1. Both allergens showed retained IgE reactivity in heat-processed orange juice. Interestingly, subjects with and without clinical allergy showed a comparable sensitization profile. Profilin and germin-like proteins are major orange allergens. The potential clinical relevance of orange profilin was indicated by its strong capacity to release histamine from basophils. However, a predominant sensitization to both allergens in subjects without symptoms also indicates a high frequency of clinically insignificant sensitization.

Authors+Show Affiliations

Hospital Universitario Doce de Octubre, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16521162

Citation

Crespo, Jesus F., et al. "Germin-like Protein Cit S 1 and Profilin Cit S 2 Are Major Allergens in Orange (Citrus Sinensis) Fruits." Molecular Nutrition & Food Research, vol. 50, no. 3, 2006, pp. 282-90.
Crespo JF, Retzek M, Foetisch K, et al. Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits. Mol Nutr Food Res. 2006;50(3):282-90.
Crespo, J. F., Retzek, M., Foetisch, K., Sierra-Maestro, E., Cid-Sanchez, A. B., Pascual, C. Y., ... Scheurer, S. (2006). Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits. Molecular Nutrition & Food Research, 50(3), pp. 282-90.
Crespo JF, et al. Germin-like Protein Cit S 1 and Profilin Cit S 2 Are Major Allergens in Orange (Citrus Sinensis) Fruits. Mol Nutr Food Res. 2006;50(3):282-90. PubMed PMID: 16521162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in orange (Citrus sinensis) fruits. AU - Crespo,Jesus F, AU - Retzek,Mechthild, AU - Foetisch,Kay, AU - Sierra-Maestro,Elena, AU - Cid-Sanchez,Ana B, AU - Pascual,Cristina Y, AU - Conti,Amedeo, AU - Feliu,Angelica, AU - Rodriguez,Julia, AU - Vieths,Stefan, AU - Scheurer,Stephan, PY - 2006/3/8/pubmed PY - 2006/5/25/medline PY - 2006/3/8/entrez SP - 282 EP - 90 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 50 IS - 3 N2 - Oranges are clinically relevant allergenic foods. To date, orange allergens have not been characterized in detail. The study is aimed at analyzing the sensitization profile in orange-sensitized subjects with and without clinical allergy, and to identify orange allergens. Fifty-six sensitized subjects with self-reported reactions to orange were grouped into reactors (anaphylaxis or multiple episodes of immediate reactions and/or positive challenge tests) and non-reactors (negative open food challenge tests). Allergens were characterized by IgE immunoblotting, N-terminal sequencing, IgE-inhibition assays, and mediator release assays were performed to determine the allergenic potency of orange profilin. Of 56 subjects, 23 were classified as orange allergic showing mainly an oral allergy syndrome. Of 23 subjects classified as orange allergic, 22 were sensitized to profilin, Cit s 2. In patients with mono-sensitization to profilin in vitro histamine releases up to 75% from basophils were induced using orange extract and purified plant profilins. Of the allergic patients 78% were sensitized to germin-like protein, Cit s 1. Both allergens showed retained IgE reactivity in heat-processed orange juice. Interestingly, subjects with and without clinical allergy showed a comparable sensitization profile. Profilin and germin-like proteins are major orange allergens. The potential clinical relevance of orange profilin was indicated by its strong capacity to release histamine from basophils. However, a predominant sensitization to both allergens in subjects without symptoms also indicates a high frequency of clinically insignificant sensitization. SN - 1613-4125 UR - https://www.unboundmedicine.com/medline/citation/16521162/Germin_like_protein_Cit_s_1_and_profilin_Cit_s_2_are_major_allergens_in_orange__Citrus_sinensis__fruits_ L2 - https://doi.org/10.1002/mnfr.200500200 DB - PRIME DP - Unbound Medicine ER -