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Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae.
J Biol Chem. 2006 May 05; 281(18):12308-14.JB

Abstract

In Saccharomyces cerevisiae, the heme-activated protein complex Hap2/3/4/5 plays a major role in the transcription of genes involved in respiration. Thus, overexpression of HAP4 has been shown to result in a 10% increase in the respiratory capacity. Here the physiology of a HAP4-deleted S. cerevisiae strain was investigated, and we found that the hap4delta S. cerevisiae exhibited poor growth on ethanol, although the growth rate on glucose was indifferent from the wild type in aerobic as well as anaerobic cultures. Moreover, it exhibited a large (75%) reduction in the critical glucose uptake rate at which fermentative metabolism is onset, indicating a substantial reduction in respiratory capacity. We also performed whole genome transcription analysis for the hap4delta and the wild type, grown in carbon-limited chemostat cultures operated at a dilution rate of 0.05 h(-1). Although both strains exhibited respiratory metabolism, there was significant change in expression of many genes in the hap4delta strain. These genes are involved in several different parts of the metabolism, including oxidative stress response, peroxisomal functions, and energy generation. This study strongly indicates that Hap4 activation only occurs at intermediate specific growth rates, below which the transcription of genes responsible for respiration is dependent on the Hap2/3/5 complex and above which the Hap4 protein augments the transcription. Furthermore, statistical analysis of the transcription data and integration of the data with a genome scale metabolic network provided new insight and evidence for the role of Hap4 in transcriptional regulation of mitochondrial respiration.

Authors+Show Affiliations

Center for Microbial Biotechnology, Building 223, BioCentrum-DTU, Technical University of Denmark, DK 2800, Kongens Lyngby, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16522629

Citation

Raghevendran, Vijayendran, et al. "Hap4 Is Not Essential for Activation of Respiration at Low Specific Growth Rates in Saccharomyces Cerevisiae." The Journal of Biological Chemistry, vol. 281, no. 18, 2006, pp. 12308-14.
Raghevendran V, Patil KR, Olsson L, et al. Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae. J Biol Chem. 2006;281(18):12308-14.
Raghevendran, V., Patil, K. R., Olsson, L., & Nielsen, J. (2006). Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae. The Journal of Biological Chemistry, 281(18), 12308-14.
Raghevendran V, et al. Hap4 Is Not Essential for Activation of Respiration at Low Specific Growth Rates in Saccharomyces Cerevisiae. J Biol Chem. 2006 May 5;281(18):12308-14. PubMed PMID: 16522629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae. AU - Raghevendran,Vijayendran, AU - Patil,Kiran Raosaheb, AU - Olsson,Lisbeth, AU - Nielsen,Jens, Y1 - 2006/03/07/ PY - 2006/3/9/pubmed PY - 2006/6/29/medline PY - 2006/3/9/entrez SP - 12308 EP - 14 JF - The Journal of biological chemistry JO - J Biol Chem VL - 281 IS - 18 N2 - In Saccharomyces cerevisiae, the heme-activated protein complex Hap2/3/4/5 plays a major role in the transcription of genes involved in respiration. Thus, overexpression of HAP4 has been shown to result in a 10% increase in the respiratory capacity. Here the physiology of a HAP4-deleted S. cerevisiae strain was investigated, and we found that the hap4delta S. cerevisiae exhibited poor growth on ethanol, although the growth rate on glucose was indifferent from the wild type in aerobic as well as anaerobic cultures. Moreover, it exhibited a large (75%) reduction in the critical glucose uptake rate at which fermentative metabolism is onset, indicating a substantial reduction in respiratory capacity. We also performed whole genome transcription analysis for the hap4delta and the wild type, grown in carbon-limited chemostat cultures operated at a dilution rate of 0.05 h(-1). Although both strains exhibited respiratory metabolism, there was significant change in expression of many genes in the hap4delta strain. These genes are involved in several different parts of the metabolism, including oxidative stress response, peroxisomal functions, and energy generation. This study strongly indicates that Hap4 activation only occurs at intermediate specific growth rates, below which the transcription of genes responsible for respiration is dependent on the Hap2/3/5 complex and above which the Hap4 protein augments the transcription. Furthermore, statistical analysis of the transcription data and integration of the data with a genome scale metabolic network provided new insight and evidence for the role of Hap4 in transcriptional regulation of mitochondrial respiration. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/16522629/Hap4_is_not_essential_for_activation_of_respiration_at_low_specific_growth_rates_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -