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A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization.
Appl Microbiol Biotechnol. 2006 Oct; 72(5):975-81.AM

Abstract

The role of genes involved in sucrose catabolism was investigated with a view to designing effective prebiotic substrates to encourage the growth of Bifidobacterium in the gut. Two gene clusters coding for sucrose utilisation in Bifidobacterium longum NCC2705 were identified in the published genome. The genes encoding putative sucrose degrading enzymes, namely, the scrP (sucrose phosphorylase) and the cscA (beta-fructofuranosidase), were cloned from B. longum NCIMB 702259(T) and expressed in Escherichia coli DH5alpha. Both complemented the sucrase negative phenotype of untransformed cells and showed specific sucrase activity. Transcriptional analysis of the expression of the genes in B. longum grown in the presence of various carbohydrate substrates showed induction of scrP gene expression in the presence of sucrose and raffinose, but not in the presence of glucose. The cscA gene showed no increased transcription in B. longum grown in the presence of any of the carbohydrates tested. Phylogenetic analysis indicates that the B. longum CscA protein belongs to a distinct phylogenetic cluster of intracellular fructosidases, which specifically cleave the shorter fructose oligosaccharides.

Authors+Show Affiliations

Kullin B
Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
Abratt VR
No affiliation info available
Reid SJ
No affiliation info available

MeSH

Bacterial ProteinsBifidobacteriumCulture MediaGene Expression Regulation, BacterialPhylogenyRNA, BacterialSucroseTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16523284

Citation

Kullin, B, et al. "A Functional Analysis of the Bifidobacterium Longum cscA and scrP Genes in Sucrose Utilization." Applied Microbiology and Biotechnology, vol. 72, no. 5, 2006, pp. 975-81.
Kullin B, Abratt VR, Reid SJ. A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. Appl Microbiol Biotechnol. 2006;72(5):975-81.
Kullin, B., Abratt, V. R., & Reid, S. J. (2006). A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. Applied Microbiology and Biotechnology, 72(5), 975-81.
Kullin B, Abratt VR, Reid SJ. A Functional Analysis of the Bifidobacterium Longum cscA and scrP Genes in Sucrose Utilization. Appl Microbiol Biotechnol. 2006;72(5):975-81. PubMed PMID: 16523284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A functional analysis of the Bifidobacterium longum cscA and scrP genes in sucrose utilization. AU - Kullin,B, AU - Abratt,V R, AU - Reid,S J, Y1 - 2006/03/08/ PY - 2005/12/07/received PY - 2006/01/27/accepted PY - 2006/01/26/revised PY - 2006/3/9/pubmed PY - 2006/12/21/medline PY - 2006/3/9/entrez SP - 975 EP - 81 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 72 IS - 5 N2 - The role of genes involved in sucrose catabolism was investigated with a view to designing effective prebiotic substrates to encourage the growth of Bifidobacterium in the gut. Two gene clusters coding for sucrose utilisation in Bifidobacterium longum NCC2705 were identified in the published genome. The genes encoding putative sucrose degrading enzymes, namely, the scrP (sucrose phosphorylase) and the cscA (beta-fructofuranosidase), were cloned from B. longum NCIMB 702259(T) and expressed in Escherichia coli DH5alpha. Both complemented the sucrase negative phenotype of untransformed cells and showed specific sucrase activity. Transcriptional analysis of the expression of the genes in B. longum grown in the presence of various carbohydrate substrates showed induction of scrP gene expression in the presence of sucrose and raffinose, but not in the presence of glucose. The cscA gene showed no increased transcription in B. longum grown in the presence of any of the carbohydrates tested. Phylogenetic analysis indicates that the B. longum CscA protein belongs to a distinct phylogenetic cluster of intracellular fructosidases, which specifically cleave the shorter fructose oligosaccharides. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/16523284/A_functional_analysis_of_the_Bifidobacterium_longum_cscA_and_scrP_genes_in_sucrose_utilization_ DB - PRIME DP - Unbound Medicine ER -