Tags

Type your tag names separated by a space and hit enter

Simultaneous determination of three local anesthetic drugs from the pipecoloxylidide group in human serum by high-performance liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 13; 834(1-2):213-6.JC

Abstract

A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous analysis of the local anesthetic amide drugs, bupivacaine, mepivacaine and ropivacaine, belonging to the pipecoloxylidide group using a C(18) reversed-phase column (150 x 4.6 mm I.D.) filled with 5-microm particles and attached to a UV detector. The mobile phase was composed of acetonitrile-methanol-30 mM NaH(2)PO(4) (pH 5.6) (100:100:300, v/v/v) and the flow rate was 1ml/min. The absorbance of the eluate was monitored at 210 nm. The retention times of the three compounds were: 4.6 min (mepivacaine), 9.7min (ropivacaine) and 16.4 min (bupivacaine). With this sample preparation method, good and consistent recoveries of the three compounds were obtained: 88-91% for mepivacaine, 87-89% for ropivacaine and 88-91% for bupivacaine. The limit of quantification for three compounds in human serum was 2 ng/ml for mepivacaine, 5 ng/ml for bupivacaine and ropivacaine. This method may be useful in clinical and forensic applications for the determination or identification of the local anesthetic drugs: bupivacaine, mepivacaine or ropivacaine.

Authors+Show Affiliations

Institute of Community Medicine, University of Tsukuba, Ibaraki-ken 305-8575, Japan. einosuke@md.tsukuba.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16530026

Citation

Tanaka, Einosuke, et al. "Simultaneous Determination of Three Local Anesthetic Drugs From the Pipecoloxylidide Group in Human Serum By High-performance Liquid Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 834, no. 1-2, 2006, pp. 213-6.
Tanaka E, Nakamura T, Inomata S, et al. Simultaneous determination of three local anesthetic drugs from the pipecoloxylidide group in human serum by high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;834(1-2):213-6.
Tanaka, E., Nakamura, T., Inomata, S., & Honda, K. (2006). Simultaneous determination of three local anesthetic drugs from the pipecoloxylidide group in human serum by high-performance liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 834(1-2), 213-6.
Tanaka E, et al. Simultaneous Determination of Three Local Anesthetic Drugs From the Pipecoloxylidide Group in Human Serum By High-performance Liquid Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 13;834(1-2):213-6. PubMed PMID: 16530026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of three local anesthetic drugs from the pipecoloxylidide group in human serum by high-performance liquid chromatography. AU - Tanaka,Einosuke, AU - Nakamura,Takako, AU - Inomata,Shinichi, AU - Honda,Katsuya, Y1 - 2006/03/09/ PY - 2005/11/01/received PY - 2006/01/30/revised PY - 2006/02/17/accepted PY - 2006/3/15/pubmed PY - 2006/6/29/medline PY - 2006/3/15/entrez SP - 213 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 834 IS - 1-2 N2 - A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous analysis of the local anesthetic amide drugs, bupivacaine, mepivacaine and ropivacaine, belonging to the pipecoloxylidide group using a C(18) reversed-phase column (150 x 4.6 mm I.D.) filled with 5-microm particles and attached to a UV detector. The mobile phase was composed of acetonitrile-methanol-30 mM NaH(2)PO(4) (pH 5.6) (100:100:300, v/v/v) and the flow rate was 1ml/min. The absorbance of the eluate was monitored at 210 nm. The retention times of the three compounds were: 4.6 min (mepivacaine), 9.7min (ropivacaine) and 16.4 min (bupivacaine). With this sample preparation method, good and consistent recoveries of the three compounds were obtained: 88-91% for mepivacaine, 87-89% for ropivacaine and 88-91% for bupivacaine. The limit of quantification for three compounds in human serum was 2 ng/ml for mepivacaine, 5 ng/ml for bupivacaine and ropivacaine. This method may be useful in clinical and forensic applications for the determination or identification of the local anesthetic drugs: bupivacaine, mepivacaine or ropivacaine. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16530026/Simultaneous_determination_of_three_local_anesthetic_drugs_from_the_pipecoloxylidide_group_in_human_serum_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00156-5 DB - PRIME DP - Unbound Medicine ER -