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Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit.
J Antimicrob Chemother. 2006 May; 57(5):979-82.JA

Abstract

OBJECTIVES

To investigate the molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae in the neonatal intensive care unit of a university hospital in Italy.

METHODS

Antibiotic susceptibility was evaluated by disc diffusion and Etest. ESBLs were identified by isoelectric focusing, PCR and DNA sequencing analysis. Genotyping was performed by PFGE analysis. Conjugation was performed by broth mating.

RESULTS

Molecular typing of K. pneumoniae isolates identified three distinct PFGE patterns. Isolates of PFGE profile A were isolated during an epidemic in 1996, while isolates of PFGE profiles B and C were sequentially isolated from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. All K. pneumoniae strains of different PFGE types were identified as ESBL producers. DNA sequencing of amplified beta-lactamase genes identified a novel bla(TEM) ESBL (bla(TEM-136)) along with bla(SHV-1) in chromosomal and plasmid DNA from K. pneumoniae of PFGE type A, respectively, and bla(TEM-1) and bla(SHV-12) in plasmid DNA from K. pneumoniae of PFGE types B and C. Conjugation experiments demonstrated that resistance to third-generation cephalosporins, along with an approximately 80 kb plasmid containing bla(SHV-12) and bla(TEM-1), was transferred from K. pneumoniae epidemic strains of PFGE types B and C to a susceptible Escherichia coli host at a frequency of 4 x 10(-6) and 1 x 10(-6) cfu/recipient cell, respectively.

CONCLUSIONS

The selection of ESBL-producing clones and the transfer of the bla(SHV-12) ESBL gene between different clones were responsible for the spread of K. pneumoniae in the neonatal intensive care unit.

Authors+Show Affiliations

Dipartimento di Scienze Mediche Preventive, Sezione di Igiene, Università di Napoli Federico II, Napoli, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16531430

Citation

Bagattini, Maria, et al. "Molecular Epidemiology of Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae in a Neonatal Intensive Care Unit." The Journal of Antimicrobial Chemotherapy, vol. 57, no. 5, 2006, pp. 979-82.
Bagattini M, Crivaro V, Di Popolo A, et al. Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit. J Antimicrob Chemother. 2006;57(5):979-82.
Bagattini, M., Crivaro, V., Di Popolo, A., Gentile, F., Scarcella, A., Triassi, M., Villari, P., & Zarrilli, R. (2006). Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit. The Journal of Antimicrobial Chemotherapy, 57(5), 979-82.
Bagattini M, et al. Molecular Epidemiology of Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae in a Neonatal Intensive Care Unit. J Antimicrob Chemother. 2006;57(5):979-82. PubMed PMID: 16531430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit. AU - Bagattini,Maria, AU - Crivaro,Valeria, AU - Di Popolo,Anna, AU - Gentile,Fabrizio, AU - Scarcella,Alda, AU - Triassi,Maria, AU - Villari,Paolo, AU - Zarrilli,Raffaele, Y1 - 2006/03/10/ PY - 2006/3/15/pubmed PY - 2006/7/6/medline PY - 2006/3/15/entrez SP - 979 EP - 82 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 57 IS - 5 N2 - OBJECTIVES: To investigate the molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae in the neonatal intensive care unit of a university hospital in Italy. METHODS: Antibiotic susceptibility was evaluated by disc diffusion and Etest. ESBLs were identified by isoelectric focusing, PCR and DNA sequencing analysis. Genotyping was performed by PFGE analysis. Conjugation was performed by broth mating. RESULTS: Molecular typing of K. pneumoniae isolates identified three distinct PFGE patterns. Isolates of PFGE profile A were isolated during an epidemic in 1996, while isolates of PFGE profiles B and C were sequentially isolated from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. All K. pneumoniae strains of different PFGE types were identified as ESBL producers. DNA sequencing of amplified beta-lactamase genes identified a novel bla(TEM) ESBL (bla(TEM-136)) along with bla(SHV-1) in chromosomal and plasmid DNA from K. pneumoniae of PFGE type A, respectively, and bla(TEM-1) and bla(SHV-12) in plasmid DNA from K. pneumoniae of PFGE types B and C. Conjugation experiments demonstrated that resistance to third-generation cephalosporins, along with an approximately 80 kb plasmid containing bla(SHV-12) and bla(TEM-1), was transferred from K. pneumoniae epidemic strains of PFGE types B and C to a susceptible Escherichia coli host at a frequency of 4 x 10(-6) and 1 x 10(-6) cfu/recipient cell, respectively. CONCLUSIONS: The selection of ESBL-producing clones and the transfer of the bla(SHV-12) ESBL gene between different clones were responsible for the spread of K. pneumoniae in the neonatal intensive care unit. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16531430/Molecular_epidemiology_of_extended_spectrum_beta_lactamase_producing_Klebsiella_pneumoniae_in_a_neonatal_intensive_care_unit_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkl077 DB - PRIME DP - Unbound Medicine ER -