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Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants.
Plant Physiol. 2006 Jun; 141(2):475-87.PP

Abstract

The role of mitogen-activated protein kinase (MAPK) in abscisic acid (ABA)-induced antioxidant defense was investigated in leaves of maize (Zea mays) plants. Treatments with ABA or H(2)O(2) induced the activation of a 46-kD MAPK and enhanced the expression of the antioxidant genes CAT1, cAPX, and GR1 and the total activities of the antioxidant enzymes catalase, ascorbate peroxidase, glutathione reductase, and superoxide dismutase. Such enhancements were blocked by pretreatment with several MAPK kinase inhibitors and reactive oxygen species inhibitors or scavengers. Pretreatment with MAPK kinase inhibitors also substantially arrested the ABA-induced H(2)O(2) production after 2 h of ABA treatment, but did not affect the levels of H(2)O(2) within 1 h of ABA treatment. Pretreatment with several inhibitors of protein tyrosine phosphatase, which is believed to be a negative regulator of MAPK, only slightly prevented the ABA-induced H(2)O(2) production, but did not affect the ABA-induced MAPK activation and ABA-enhanced antioxidant defense systems. These results clearly suggest that MAPK but not protein tyrosine phosphatase is involved in the ABA-induced antioxidant defense, and a cross talk between H(2)O(2) production and MAPK activation plays a pivotal role in the ABA signaling. ABA-induced H(2)O(2) production activates MAPK, which in turn induces the expression and the activities of antioxidant enzymes. The activation of MAPK also enhances the H(2)O(2) production, forming a positive feedback loop.

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Authors+Show Affiliations

Zhang A
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Jiang M
No affiliation info available
Zhang J
No affiliation info available
Tan M
No affiliation info available
Hu X
No affiliation info available

MeSH

Abscisic AcidAntioxidantsBase SequenceCatalaseDNA PrimersEnzyme InhibitorsGlutathione ReductaseHydrogen PeroxideImmunoprecipitationMitogen-Activated Protein KinasesPlant LeavesReactive Oxygen SpeciesSignal TransductionSuperoxide DismutaseZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16531486

Citation

Zhang, Aying, et al. "Mitogen-activated Protein Kinase Is Involved in Abscisic Acid-induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants." Plant Physiology, vol. 141, no. 2, 2006, pp. 475-87.
Zhang A, Jiang M, Zhang J, et al. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiol. 2006;141(2):475-87.
Zhang, A., Jiang, M., Zhang, J., Tan, M., & Hu, X. (2006). Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiology, 141(2), 475-87.
Zhang A, et al. Mitogen-activated Protein Kinase Is Involved in Abscisic Acid-induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants. Plant Physiol. 2006;141(2):475-87. PubMed PMID: 16531486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. AU - Zhang,Aying, AU - Jiang,Mingyi, AU - Zhang,Jianhua, AU - Tan,Mingpu, AU - Hu,Xiuli, Y1 - 2006/03/10/ PY - 2006/3/15/pubmed PY - 2006/8/29/medline PY - 2006/3/15/entrez SP - 475 EP - 87 JF - Plant physiology JO - Plant Physiol VL - 141 IS - 2 N2 - The role of mitogen-activated protein kinase (MAPK) in abscisic acid (ABA)-induced antioxidant defense was investigated in leaves of maize (Zea mays) plants. Treatments with ABA or H(2)O(2) induced the activation of a 46-kD MAPK and enhanced the expression of the antioxidant genes CAT1, cAPX, and GR1 and the total activities of the antioxidant enzymes catalase, ascorbate peroxidase, glutathione reductase, and superoxide dismutase. Such enhancements were blocked by pretreatment with several MAPK kinase inhibitors and reactive oxygen species inhibitors or scavengers. Pretreatment with MAPK kinase inhibitors also substantially arrested the ABA-induced H(2)O(2) production after 2 h of ABA treatment, but did not affect the levels of H(2)O(2) within 1 h of ABA treatment. Pretreatment with several inhibitors of protein tyrosine phosphatase, which is believed to be a negative regulator of MAPK, only slightly prevented the ABA-induced H(2)O(2) production, but did not affect the ABA-induced MAPK activation and ABA-enhanced antioxidant defense systems. These results clearly suggest that MAPK but not protein tyrosine phosphatase is involved in the ABA-induced antioxidant defense, and a cross talk between H(2)O(2) production and MAPK activation plays a pivotal role in the ABA signaling. ABA-induced H(2)O(2) production activates MAPK, which in turn induces the expression and the activities of antioxidant enzymes. The activation of MAPK also enhances the H(2)O(2) production, forming a positive feedback loop. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/16531486/Mitogen_activated_protein_kinase_is_involved_in_abscisic_acid_induced_antioxidant_defense_and_acts_downstream_of_reactive_oxygen_species_production_in_leaves_of_maize_plants_ DB - PRIME DP - Unbound Medicine ER -